Comparison of plasma and chemical modifications of poly-L-lactide-co-caprolactone scaffolds for heparin conjugation

Hsieh, Yu-Fang; Sahagian, Khoren; Huang, Fu-Ying; Xu, Kang; Patel, Shyam; Li, Song

doi:10.1088/1748-605x/aa81aa

Cited by 15 publications

(18 citation statements)

References 46 publications

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“…In such a case, only one of the amino groups is consumed during the chain scission at the polymer surface, being converted to an amide group, while the second amine group remains free. This method is based on simple treating of the polymer scaffold in diamine solution (e.g., 1,6-hexanediamine [21], 1,2-ethanediamine [22], Fmoc-PEG-diamine [23], etc.) and was previously considered for improving the dyeing process and moisture regain in the textile industry [24,25,26].…”

Section: Introductionmentioning

confidence: 99%

Aminolysis of Various Aliphatic Polyesters in a Form of Nanofibers and Films

Jeznach

Kołbuk

2019

Polymers

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Surface functionalization of polymer scaffolds is a method used to improve interactions of materials with cells. A frequently used method for polyesters is aminolysis reaction, which introduces free amine groups on the surface. In this study, nanofibrous scaffolds and films of three different polyesters–polycaprolactone (PCL), poly(lactide-co-caprolactone) (PLCL), and poly(l-lactide) (PLLA) were subjected to this type of surface modification under the same conditions. Efficiency of aminolysis was evaluated on the basis of ninhydrin tests and ATR–FTIR spectroscopy. Also, impact of this treatment on the mechanical properties, crystallinity, and wettability of polyesters was compared and discussed from the perspective of aminolysis efficiency. It was shown that aminolysis is less efficient in the case of nanofibers, particularly for PCL nanofibers. Our hypothesis based on the fundamentals of classical high speed spinning process is that the lower efficiency of aminolysis in the case of nanofibers is associated with the radial distribution of crystallinity of electrospun fiber with more crystalline skin, strongly inhibiting the reaction. Moreover, the water contact angle results demonstrate that the effect of free amino groups on wettability is very different depending on the type and the form of polymer. The results of this study can help to understand fundamentals of aminolysis-based surface modification.

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Section: Introductionmentioning

confidence: 99%

Aminolysis of Various Aliphatic Polyesters in a Form of Nanofibers and Films

Jeznach

Kołbuk

2019

Polymers

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“…As the stability and activity of heparin immobilized on vascular grafts are critical for blood compatibility in vivo, methods for heparin coating of vascular graft polymers have ranged from the adsorption of heparin from aqueous solution to covalent end-point immobilization [25]. It was reported that the hydrophilicity of PCL was improved after combination with heparin [18, 26]. In this study, we described a novel method for immobilizing heparin on PCL scaffolds via HMD, and PCL-HMD-H scaffold was successfully prepared by the electrospinning technique and surface modification.…”

Section: Discussionmentioning

confidence: 99%

Vascular Remodeling Process of Heparin-Conjugated Poly(ε-Caprolactone) Scaffold in a Rat Abdominal Aorta Replacement Model

et al. 2018

J Vasc Res

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In the field of vascular graft research, poly-ε-caprolactone (PCL) is used owing to its good mechanical strength and biocompatibility. In this study, PCL scaffold was prepared by electrospinning and surface modification with heparin via hexamethylenediamine. Then the scaffolds were implanted into the infrarenal abdominal aorta of Wistar rats and contrast-enhanced micro-ultrasound was used to monitor the patency of grafts after implantation. These grafts were extracted from the rats at 1, 3, and 6 months for histological analysis, immunofluorescence staining, and scanning electron microscopy observation. Although some grafts experienced aneurysmal change, results showed that all implanted grafts were patent during the course of 6 months and these grafts demonstrated well-organized neotissue with endothelium formation, smooth muscle regeneration, and extracellular matrix formation. Such findings confirm feasibility to create heparin-conjugated scaffolds of next-generation vascular grafts.

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“…A two-stage (ammonia followed by hydrogen) plasma treatment was used to introduce amine functional groups on the surface of the PLCL/PDO blend grafts [ 23 , 24 ]. The untreated and plasma-treated grafts were incubated with a heparin solution (Sigma Aldrich) in cyanoborohydride coupling buffer overnight.…”

Section: Methodsmentioning

confidence: 99%

“…In brief, control and heparinized grafts were incubated in diH 2 O for 1 and 7 days at room temperature on a shaker. At the end of day 1 and 7, the activity of the heparin remaining on the grafts was quantified with the Anti-thrombin-III assay following a previously described protocol [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Engineering the Composition of Microfibers to Enhance the Remodeling of a Cell-Free Vascular Graft

et al. 2021

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The remodeling of vascular grafts is critical for blood vessel regeneration. However, most scaffold materials have limited cell infiltration. In this study, we designed and fabricated a scaffold that incorporates a fast-degrading polymer polydioxanone (PDO) into the microfibrous structure by means of electrospinning technology. Blending PDO with base polymer decreases the density of electrospun microfibers yet did not compromise the mechanical and structural properties of the scaffold, and effectively enhanced cell infiltration. We then used this technique to fabricate a tubular scaffold with heparin conjugated to the surface to suppress thrombosis, and the construct was implanted into the carotid artery as a vascular graft in animal studies. This graft significantly promoted cell infiltration, and the biochemical cues such as immobilized stromal cell-derived factor-1α further enhanced cell recruitment and the long-term patency of the grafts. This work provides an approach to optimize the microfeatures of vascular grafts, and will have broad applications in scaffold design and fabrication for regenerative engineering.

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Comparison of plasma and chemical modifications of poly-L-lactide-co-caprolactone scaffolds for heparin conjugation

Cited by 15 publications

References 46 publications

Aminolysis of Various Aliphatic Polyesters in a Form of Nanofibers and Films

Aminolysis of Various Aliphatic Polyesters in a Form of Nanofibers and Films

Vascular Remodeling Process of Heparin-Conjugated Poly(ε-Caprolactone) Scaffold in a Rat Abdominal Aorta Replacement Model

Engineering the Composition of Microfibers to Enhance the Remodeling of a Cell-Free Vascular Graft

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