Comparison of Plasma Metabolite Concentrations and Peripheral Leukocyte Enzyme Activities in Sheep Fed on Different Diets in New Zealand and Japan

Arai, Toshiro; Kusaba, A.; Takeguchi, A.; Tanaka, Yoshikazu; Ozawa, Tomomi; Yoshida, Tatsuyuki; Sako, Toshinori; Hayashi, Tomohito; Blair, Hugh T.

doi:10.1007/s11259-007-3548-9

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…LDH is an enzyme that catalyzes the conversion of lactate to Pyruvate; an important step in energy production in cells. Some of the organs relatively rich in LDH are the heart, kidney, liver, and muscles3. Because this enzyme is actually composed of five different isoenzymes, however, analysis of the different LDH isoenzyme levels in the blood can help in the diagnosis of some diseases.…”

Section: Introductionmentioning

confidence: 99%

Thyroid profile and LDH Isoenzymes as prognostic biomarkers for diabetic and/or obese subjects

Johari¹,

Ghoneim²,

Moselhy³

2018

Afr H. Sci.

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BackgroundThis study aimed to evaluate the levels of thyroid hormones and lactate dehydrogenase (LDH) isoenzymes in obese and/or diabetic patients.Subjects and methodsForty male subjects categorized into four equal groups; group 1: Non obese control subjects, group 2: Subjects suffering from type 2 diabetes mellitus (T2DM), group 3: Obese subjects (BMI ≥ 30 kg/m2) and group 4: Subjects thatwere obese and had type 2 diabetes mellitus (T2DM). Liver, kidney, lipid, thyroid hormones, total LDH and LDH isoenzymes levels were determined.ResultsThere was a significant increase of TSH level (p<0.001) in diabetic group as compared with control group and a highly significant increase of TSH was obtained in obese and obese diabetic groups versus control and diabetic patients. LDH 2 was also highly significantly decreased in obese and obese diabetic groups versus diabetic patients. Percentage of LDH 4 was significantly decreased in both diabetic and obese groups and not significantly changed in obese diabetic patients as compared with the control group. LDH 5 percentage showed very highly significant decrease in diabetic, obese and highly significant decrease in obese diabetic groups when compared with control subjects while it was not significantly changed in obese and obese diabetic groups as compared with diabetic patients.ConclusionLDH isozymes can be used as valuable diagnostic markers for metabolic syndrome. This may help to explore the metabolic changes associated with obesity and diabetes complication and following up the complication of these abnormalities.

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Section: Introductionmentioning

confidence: 99%

Thyroid profile and LDH Isoenzymes as prognostic biomarkers for diabetic and/or obese subjects

Johari¹,

Ghoneim²,

Moselhy³

2018

Afr H. Sci.

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“…Diet containing high level of carbohydrates causes change in the concentration of some metabolites related to glucose and lipid metabolism (Arai et al, 2007). Therefore, activities of enzymes related to energy metabolism and concentration of metabolites as substrates or end products of energy metabolism may become the important parameters for evaluating the metabolic state of farm animals fed different diets (Arai et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Impact of probiotic-supplemented diet on the expression level of lactate dehydrogenase in the leukocytes of rabbits

Ghoneim

Moselhy

2012

Toxicol Ind Health

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Probiotics are known as living, nonpathogenic microorganisms that colonize the intestine and provide benefit to the host. The present study aims to measure one important energy metabolism-related enzyme activity in blood of rabbits fed on probiotics of recommended concentration. In addition, it also aims for the evaluation of the expression level of lactate dehydrogenase (LDH) enzyme using reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Two groups of rabbits are used: control group receiving normal standardized diet and the other probiotic-supplemented group receiving the same diet containing probiotic, namely, Mega acidophilus (200 million cfu/kg body weight/day) for 4 weeks. The obtained results revealed that the rabbits supplemented with probiotics showed a significant decrease in the levels of serum total cholesterol (TC), triacylglycerol, high-density lipoprotein cholesterol (HDL-c) and low-density lipoprotein cholesterol (LDL-c) when compared with control group. Risk factors detected by measuring TC/HDL-c and LDL-c/HDL-c ratios showed statistically significant decrease in probiotic-supplemented rabbits when compared with control group. In addition, blood glucose and total LDH activity were elevated in probiotic-supplemented rabbits when compared with control group. RT-PCR products of LDH-M gene produced two specific amplicons. One amplicon has the expected size of 243 bp from all samples of rabbits as revealed by GelPro software. The level of LDH-M expression was found to be increased in the probiotic-supplemented group. However, unexpected amplicons are produced at 586 bp in all the samples, which may be a dimeric form of the amplified region. It was concluded that this probiotic blend is beneficiary for the metabolic reactions of lipids in the body. Moreover, LDH expression level can be considered as a biomarker for the effect of probiotic and hence monitoring the metabolic changes as reflected from its administration.

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“…Sabendo-se que a alimentação pode influenciar a concentração dos metabólitos plasmáticos, Arai et al (2007)…”

Section: Suplementação Mineralunclassified

Revisão da literatura: mineralização e perfil metabólico em ovinos

Ortunho¹

2015

Pubvet

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A ovinocultura é uma atividade que vem crescendo nos últimos anos, porém na literatura são poucas as pesquisas que reúnem dados sanguíneos e que reúnem dados sobre a mineralização destes animais, tanto com minerais orgânicos e quelatados. Baseado nisso foi feito um levantamento com os trabalhos mais atuais sobre mineralização, balanço energético negativo e estudo de perfil metabólico de animais em diferentes fases reprodutivas e produtivas desta espécie, o que originou esta revisão da literatura.

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Comparison of Plasma Metabolite Concentrations and Peripheral Leukocyte Enzyme Activities in Sheep Fed on Different Diets in New Zealand and Japan

Cited by 5 publications

References 12 publications

Thyroid profile and LDH Isoenzymes as prognostic biomarkers for diabetic and/or obese subjects

Thyroid profile and LDH Isoenzymes as prognostic biomarkers for diabetic and/or obese subjects

Impact of probiotic-supplemented diet on the expression level of lactate dehydrogenase in the leukocytes of rabbits

Revisão da literatura: mineralização e perfil metabólico em ovinos

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