Comparison of platelet counts by CellDyn Sapphire (Abbot Diagnostics), LH750 (Beckman Coulter), ReaPanThrombo immunoplatelet method (ReaMetrix), and the international flow reference method, in thrombocytopenic blood samples

Sehgal, Kunal; Badrinath, Yajamanam; Tembhare, Prashant; Subramanian, Papagudi Ganesan; Talole, Sanjay; Kumar, Ashok; Gadage, Vijaya; Mahadik, Shashikant; Ghogale, Sitaram; Gujral, Sumeet

doi:10.1002/cyto.b.20515

Cited by 7 publications

(2 citation statements)

References 19 publications

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“…Thus, many instrument manufacturers have applied a variety of measures to solve this problem. Advia 120, Cell-Dyn 4000, Mindray BC-6800 and Abbot Diagnostics are platelet counters using the optical counting method that can differentiate blood components according to the type and strength of light signals [4–6]. Another analyzer, the Sysmex XN-3000, is designed to use the method of fluorescent nucleic acid staining to count platelets [7].…”

Section: Introductionmentioning

confidence: 99%

Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Sun

Huang

et al. 2019

PLoS ONE

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Objective The aim of this study is to evaluate the performance of different platelet counting methods (optical, impedance, fluorescence and hand counting) applied in different analysers by comparing with the international flow cytometric reference method (IRM). Methods A total of 333 blood samples from different subgroups (168 cases with thrombocytopenia, 136 cases with normal platelet counts and 29 cases with thrombocytosis) were tested. Regarding IRM as the gold standard, we compared the accuracy and precision of different platelet count methods; i.e. LH780 (impedance), BC-6000 Plus (optical (O) and impedance (I)), Sysmex XN-9000 (optical (O), impedance (I), fluorescence (F)), and hand counting. Results Sysmex XN-9000-F (r = 0.988) had the best correlation with IRM for thrombocytopenic samples; BC-6000 Plus-I (r = 0.966) was more relevant to IRM than any other method for samples with normal platelet counts. Correlation between Sysmex XN-9000-I (r = 0.960) and IRM was the highest among these methods for samples with thrombocytosis. For bias evaluation, the average bias of Sysmex XN-9000-F was -1.5 × 10 9 /L (95% LA = -9.4 to +6.4) for samples with thrombocytopenia, compared with IRM. BC-6000 Plus-I had a small mean difference with IRM for samples with normal platelet counts or thrombocytosis. Moreover, all evaluated methods had acceptable sensitivity, specificity, and concordance rates as compared with IRM in the diagnosis of thrombocytopenia and thrombocytosis. Conclusions Platelet counting by Sysmex XN-9000-F is more accurate than other methods for thrombocytopenic samples. BC-6000 Plus-I has superior association and consistency for normal platelet counts. As for thrombocytosis patients, Sysmex XN-9000-I has the highest correlation with IRM while Sysmex XN-9000-O has the highest diagnosis efficacy.

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Section: Introductionmentioning

confidence: 99%

Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Sun

Huang

et al. 2019

PLoS ONE

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“…Even when the value of prophylactic therapy is accepted the platelet count threshold at which this is justifi ed is still very much a matter of debate and it is certainly true that not all patients with severe thrombocytopenia should be considered autothomatic candidates for prophylactic therapy [37,38,39].…”

Section: Discussionmentioning

confidence: 99%

Chemotherapy induced thrombocytopenia treated by four types of platelets concentrates

Nikolić¹,

Nedeljkovic²,

Jelić³

et al. 2015

Hosp Pharm Int Multi J

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SUMMARYIntroduction: Serious adverse event of anticancer chemotherapy is glanulocytopenia and thrombocytopenia which can decrease effi ciency of fi nal therapy results. After many years, platelet concentrates transfusion (PCT) is still researching problem without sure standpoint. The aim: To determine whether there is a diff erence in the clinical effi ciency in the use of 4 types of platelet applied for transfusion; -to ascertain whether platelet count increase expressed as corrected count increment (CCI), is a better parameter for the evaluation of platelet transfusion effi ciency than the bleeding time (Bt), as the only readily assessable in vivo platelet function related parameter. Subjects and methods: This paper is a part of academic (noncommercial) IV phase observational nointervetion study. Investigation included 78 patients diagnosed with malignant lymphoma and metastatic solid tumors, transfused by platelet concentrates. Patients were devided into 4 groups, based on the type of platelet concentrates used for transfusion. Results: Patients, were transfused with total number of 647 PC units (235 units were non-leukodepleted and 412 units were leukodepleted). Mean number of PC transfusions per patient was 8.3 PC units, and 4.8 PC unit per one transfusion episode. Before PCT: platelets values were: 18

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Flow Cytometry

Carubbi¹,

Masselli²,

Vitale³

2017

Platelets in Thrombotic and Non-Thrombotic Disorders

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Comparison of platelet counts by CellDyn Sapphire (Abbot Diagnostics), LH750 (Beckman Coulter), ReaPanThrombo immunoplatelet method (ReaMetrix), and the international flow reference method, in thrombocytopenic blood samples

Cited by 7 publications

References 19 publications

Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Chemotherapy induced thrombocytopenia treated by four types of platelets concentrates

Flow Cytometry

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