Comparison of protective media and freezing techniques for cryopreservation of human semen

Stanić, Patrik; Tandara, Marijan; Sonicki, Zdenko; Šimunić, Velimir; Radaković, Branko; Suchanek, Ernest

doi:10.1016/s0301-2115(99)00255-9

Cited by 89 publications

(69 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…7 It has been reported that several damaging processes can occur during the freezing and thawing of human spermatozoa due to thermal shock with the formation of intracellular and extracellular ice crystals, cellular dehydration, and osmotic shock. 8 The intracellular ice crystals thus formed, may breach the membranes and affect the functioning of the organelles. This can lead to impaired cell survival.…”

Section: Discussionmentioning

confidence: 99%

Study of human sperm motility post cryopreservation

Oberoi

Kumar

Talwar

2014

Medical Journal Armed Forces India

View full text Add to dashboard Cite

a b s t r a c tBackground: Cryopreservation of spermatozoa is a widely used technique to preserve the fertility of males. It can also benefit the armed forces personnel who are to be sent for long recruitments, while leaving their families behind. This study, apart from studying the effects of freezing and thawing, reveals the effect of the post thaw interval on the motility of the human spermatozoa and thus widens the insemination window period.Methods: A detailed semen analysis was carried out as per the WHO guidelines for 25 samples. The samples were then washed, analysed and frozen in liquid nitrogen. The semen samples were subsequently thawed and similarly analysed after 20 min and 40 min of thawing. This was then followed by statistical analysis of the comparative motilities.Results: Motility of sperms is found to decrease after cryopreservation. However, the study revealed that after thawing a significant increase in the motility of the sperms was noted with the progression of time (p < 0.05). Conclusion:By simulating conditions similar to the in vivo conditions for the post thaw semen samples, we can safely wait, confirm the parameters like motility and count, and then inseminate the samples instead of blindly inseminating them immediately after thawing.

show abstract

Section: Discussionmentioning

confidence: 99%

Study of human sperm motility post cryopreservation

Oberoi

Kumar

Talwar

2014

Medical Journal Armed Forces India

View full text Add to dashboard Cite

show abstract

“…Poorly motile sperm and dead sperm were discarded, and only good motile sperm were selected by [30]. Moreover, detrimental effects of freezing on spermatozoa have been reported, such as sperm mitochondria damage and sperm tail abnormality [2,31]. These lead to abnormal movement of sperm after cryopreservation, and these poorly motile sperm were eliminated by the swim-up technique.…”

Section: Discussionmentioning

confidence: 99%

Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

Petyim

Neungton

Thanaboonyawat

et al. 2014

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To evaluate whether sperm preparation (swim-up technique) before freezing improves the percentages of sperm motility, sperm viability, and non-apoptotic spermatozoa after freezing-thawing process compared with preparation after cryopreservation. Methods Semen samples from 65 infertile males were equally divided into two aliquots one of which was processed for swim-up prior to cryopreservation and one of which was processed following cryopreservation. Sperm count, motility, and apoptosis index were measured in each group. Result (s) The total sperm count and the total motile sperm count decreased after thawing in both the pre-preparation and non-preparation groups compared with neat semen group (P<0.001). Moreover, the percentage of apoptotic sperm in the pre-preparation group after cryopreservation was lower than that in the non-preparation group (P<0.05), whereas the percentage of vital sperm with progressive motility was higher than that in the pre-preparation group (P<0.001). Conclusion (s) Semen preparation by swim-up before freezing resulted in better sperm quality and fewer apoptotic sperm than sperm preparation after thawing. Therefore, sperm preparation before cryopreservation should be considered in routine sperm cryopreservation.

show abstract

“…Several studies have shown that DNA damage is linked to changes in the morphology of frozen sperm samples, although this does not appear to be true according to studies done by Donnelly et al [2001a]. Stanic et al [2000] found that the use of slow programmable freezing was superior to vapor freezing and rapid programmable freezing for high quality semen samples. However, all of these freezing protocols produced notable changes in sperm cell motility as well as other parameters [Stanic et al 2000].…”

Section: Effects Of Cryopreservationmentioning

confidence: 99%

“…Stanic et al [2000] found that the use of slow programmable freezing was superior to vapor freezing and rapid programmable freezing for high quality semen samples. However, all of these freezing protocols produced notable changes in sperm cell motility as well as other parameters [Stanic et al 2000]. In contrast, other studies have shown that the conventional rapid freezing technique is a better method for preserving sperm motility than the manual slow technique.…”

Section: Effects Of Cryopreservationmentioning

confidence: 99%

Effect of sperm storage and selection techniques on sperm parameters

Sharma

Kattoor

Ghulmiyyah

et al. 2014

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

Sperm cryopreservation preserves the fertility of cancer patients undergoing chemotherapy, ensures sperm are available at the time of oocyte retrieval in assisted reproductive technology (ART) procedures and avoids the need for repeated sperm extraction surgeries in azoospermic patients. Conventional methods of cryopreservation involve storage in liquid nitrogen (LN 2 ), which causes a significant decline in sperm parameters such as motility and viability and results in DNA damage. Newer methods of sperm cryopreservation such as the LN 2 vapor method, vitrification, and experimental methods such as lyophilization, have significant advantages over the conventional methods in terms of cost effectiveness and ease of use. Density gradient centrifugation (DGC), swim up, and magnetic assisted cell sorting (MACS) can be used prior to or post-cryopreservation to improve post-thaw sperm quality. Cryopreservation in special carriers such as cryoloops and empty zona prevents the loss of small numbers of sperm during cryopreservation. This article will discuss these sperm preservation and selection techniques.

show abstract

Comparison of protective media and freezing techniques for cryopreservation of human semen

Cited by 89 publications

References 28 publications

Study of human sperm motility post cryopreservation

Study of human sperm motility post cryopreservation

Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

Effect of sperm storage and selection techniques on sperm parameters

Contact Info

Product

Resources

About