Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

Abstract

Purpose To evaluate whether sperm preparation (swim-up technique) before freezing improves the percentages of sperm motility, sperm viability, and non-apoptotic spermatozoa after freezing-thawing process compared with preparation after cryopreservation. Methods Semen samples from 65 infertile males were equally divided into two aliquots one of which was processed for swim-up prior to cryopreservation and one of which was processed following cryopreservation. Sperm count, motility, and apoptosis index were measured in each group. Result (s) The total sperm count and the total motile sperm count decreased after thawing in both the pre-preparation and non-preparation groups compared with neat semen group (P<0.001). Moreover, the percentage of apoptotic sperm in the pre-preparation group after cryopreservation was lower than that in the non-preparation group (P<0.05), whereas the percentage of vital sperm with progressive motility was higher than that in the pre-preparation group (P<0.001). Conclusion (s) Semen preparation by swim-up before freezing resulted in better sperm quality and fewer apoptotic sperm than sperm preparation after thawing. Therefore, sperm preparation before cryopreservation should be considered in routine sperm cryopreservation.

Cryopreservation and hypothermic storage of lacrimal gland: towards enabling delivery of regenerative medicine therapies for treatment of dry eye syndrome

“…This is clearly advantageous in terms of facilitating reconstruction of lacrimal gland tissue, as it significantly relieves some of the temporal pressures of just‐in‐time manufacture. Moreover, that we used a relatively crude method of cryopreservation without attempting to deliberately limit known cryopreservation‐induced damaging processes such as supercooling (Diener et al , ), oxidative stress (Gale et al , ; Martin Munoz et al , ) or apoptosis (Petyim et al , ) specifically, is further suggestive of the robustness of lacrimal gland epithelial cells.…”

The Impact of Reproductive Technologies on Stallion Mitochondrial Function

“…; Petyim et al. ). This form of sperm death appears to be dependent on the activation of an intrinsic apoptotic cascade originated in the mitochondria after unbalanced mitochondrial ROS generation (Koppers et al.…”

“…Perhaps the most important aspect is that all spermatozoa are programmed to die and that only one sperm reaches immortality through fertilization Aitken et al 2012a). Importantly, many sperm biotechnologies accelerate this pathway to sperm death (Ortega-Ferrusola et al 2008;Peña et al 2011;Balao da Silva et al 2013a;Petyim et al 2014). This form of sperm death appears to be dependent on the activation of an intrinsic apoptotic cascade originated in the mitochondria after unbalanced mitochondrial ROS generation (Koppers et al 2008;Aitken and Curry 2011).…”

Density Gradient Centrifugation Pra-freezing Mengoptimalkan Persentase Morfologi Normal Spermatozoa Pasca-thawing

“…2 Kriopreservasi spermatozoa pada umumnya digunakan sebelum dilakukan kemoterapi atau radioterapi, pada kasus oligoasthenoteratozoospermia (OAT) yang berat, gangguan ejakulasi serta pada kasus donor spermatozoa. 3 Prosedur kriopreservasi yang terdiri atas prosedur freezing dan thawing akan memberikan efek merugikan terhadap struktur dan fungsi spermatozoa, 4 yakni meningkatkan persentase spermatozoa dengan motilitas yang buruk dan morfologi abnormal, 5,6 menginduksi dekondensasi kromatin, serta meningkatkan fragmentasi DNA spermatozoa. 7 Teknik freezing yang digunakan saat ini dapat dibedakan menjadi teknik freezing konvensional dan teknik freezing mutakhir.…”

“…Penelitian lain menyimpulkan bahwa preparasi semen dengan metode swimup sebelum freezing akan meningkatkan kualitas spermatozoa pasca-thawing. 6 Fabozzi dkk. 12 telah menyimpulkan bahwa protokol direct-freezing memberikan hasil tuaian dengan viabilitas spermatozoa yang lebih baik, namun motilitas yang sama apabila dibanding dengan protokol preparasi pra-freezing.…”