Comparison of protein expression in two prostate cancer cell-lines, LNCaP and DU145, after treatment with somatostatin

Liu, Zhaoxu; Márquez, Marcela; Nilsson, Sten; Holmberg, Anders

doi:10.3892/or_00000587

Cited by 9 publications

(6 citation statements)

References 22 publications

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“…HSPs 27 and 70 are the most strongly induced after stresses such as anticancer drugs, or irradiation and other damaging conditions. It is also known that HSPs bind to and inactivate androgen receptor (AR) [31]. Thus, on the basis of our results, which show an up-regulation of HSP70, it is clear that selenium fortification of baker s yeast leads to an increase in the amount of HSP70 in the yeast, and this increased levels may be effective in inhibiting the binding of androgens to the receptor.…”

Section: Discussionsupporting

confidence: 56%

The effect of selenium enrichment on baker's yeast proteome

El‐Bayoumy¹,

Das²,

Russell³

et al. 2012

Journal of Proteomics

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The use of regular yeast (RY) and selenium-enriched yeast (SEY) as dietary supplement is of interest because the Nutritional Prevention of Cancer (NPC) trial revealed that SEY but not RY decreased the incidence of prostate cancer (PC). Using two-dimensional difference in gel electrophoresis (2D-DIGE) -tandem mass spectrometry (MS/MS) approach, we performed proteomic analysis of RY and SEY to identify proteins that are differentially expressed as a result of selenium enrichment. 2D-DIGE revealed 96 candidate protein spots that were differentially expressed (p≤0.05) between SEY and RY. The 96 spots were selected, sequenced by LC/MS/MS and 37 proteins were unequivocally identified. The 37 identified proteins were verified with ProteinProphet software and mapped to existing Gene Ontology categories. Furthermore, the expression profile of 5 of the proteins with validated or putative roles in the carcinogenesis process, and for which antibodies against human forms of the proteins are available commercially were verified by western analysis. This study provides evidence for the first time that SEY contains higher levels of Pyruvate Kinase, HSP70, and Elongation factor 2 and lower levels of Eukaryotic Translation Initiation Factor 5A-2 and Triosephosphate Isomerase than those found in RY.

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Section: Discussionsupporting

confidence: 56%

The effect of selenium enrichment on baker's yeast proteome

El‐Bayoumy¹,

Das²,

Russell³

et al. 2012

Journal of Proteomics

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“…It promotes protein import via (MIA) pathway and regulates cristae morphology and crista junctions [71]. It found to be down-regulated in prostate cancer androgen-independent (DU145) versus androgen-dependent (LNCaP) cell lines [72]. Similarly, in this study, IMMT expression was decreased in cis-resistant cell line as compared to its sensitive cell line using A2780 cell line as reference, suggesting its usefulness as biomarker for platinum resistance in ovarian cancer.…”

Section: Got1supporting

confidence: 59%

Sequenced Combinations of Cisplatin and Selected Phytochemicals Towards Overcoming Drug Resistance in Ovarian Tumour Models

Althurwi

Beale

et al. 2020

Preprint

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Background: In the present study, cisplatin, artemisinin and oleanolic acid were evaluated alone and in combination, on human ovarian A2780, A2780ZD0473R and A2780cisR cancer cell lines with aim of overcoming cisplatin resistance and side effects. Methods: Cytotoxicity was assessed by MTT reduction assay. CI values were used as a measure of combined drug effect. MALDI TOF/TOF MS/MS and 2-DE gel electrophoresis were used to identify protein biomarkers in ovarian cancer and to evaluate combination effects. Results: Synergism from combinations was dependent on concentration and sequence of administration. Generally, bolus was most synergistic. 49 proteins differently expressed by 2 ≥ fold were: CYPA, EIF5A1, Op18, p18, LDHB, P4HB, HSP7C, GRP94, ERp57, mortalin, IMMT, CLIC1, NM23, PSA3,1433Z, and HSP90B were down-regulated, whereas hnRNPA1, hnRNPA2/B1, EF2, GOT1, EF1A1, VIME, BIP, ATP5H, APG2, VINC, KPYM, RAN, PSA7, TPI, PGK1, ACTG and VDAC1 were up-regulated, while TCPA, TCPH, TCPB, PRDX6, EF1G, ATPA, ENOA, PRDX1, MCM7, GBLP, PSAT, Hop, EFTU, PGAM1, SERA and CAH2 were not-expressed in A2780cisR cells. The proteins were found to play critical roles in cell cycle regulation, metabolism and biosynthetic processes and drug resistance and detoxification. Conclusion: Results indicate that appropriately sequenced combinations of cisplatin with ART and OA may provide a means to reduce side effects and circumvent platinum resistance.

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“…Although the exact mechanisms of smsDX induced suppression of invasiveness are not currently clear, it might be due to responses to damaged mitochondrial functions. In our previous studies [7], [24], smsDX regulated mitochondrial and related proteins both in androgen dependent and independent prostate cancer cells, so the cancer-associated alterations in metabolism are possibly responsible for the cell proliferation and survival signals in recurrent prostate cancer and CRPC.…”

Section: Discussionmentioning

confidence: 87%

Somatostatin Derivative (smsDX) Targets Cellular Metabolism in Prostate Cancer Cells after Androgen Deprivation Therapy

et al. 2013

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Cancer cell metabolism responsive to androgen deprivation therapy (ADT) may be involved in the development and progression of prostate cancer and the ultimate failure of androgen-deprivation therapy. To investigate the metabolism regulation effects on androgen-independent growth of prostate cancer, an established LNCaP-s cell model that resembles the clinical scenario of castration-resistant prostate cancer (CRPC), was used in this current study. This cell line was cultured from androgen-sensitive LNCaP parental cells, in an androgen-reduced condition, resembling clinical androgen deprivation therapy. To assess the effects of smsDX on the invasiveness of prostate cancer cells we used wound healing assay and Matrigel™ invasion assay. We evaluated differentially expressed proteins of the parental LNCaP cells and LNCaP-s cells after ADT by means of two-dimensional gel electrophoresis (2-DE) followed by MALDI-TOF mass spectrometric analysis. The covered area in the wound and the number of cells invading through a Matrigel chamber were significantly smaller for cells treated with smsDX than they were for control cells treated with vehicle. 56 proteins were found differentially expressed in LNCaP-s cells compared to LNCaP cells, majority of them were down-regulated after ADT treatment. 104 proteins of LNCaP cells and 86 in LNCaP-s cells, separately, were found differentially expressed after treatment with smsDX, When we explored these protein functions within the website UniProtKB/Swiss-Prot, surprisingly, most of the proteins were found to be involved in the cellular metabolism and mitochondrial function regulation. LNCaP-s as potential metastatic androgen-independent cancer cells, its metabolism and mitochondrial functions could be altered by a new somatostatin derivative smsDX, the smsDX regulatory effects on metabolism in LNCaP-s deliver more therapeutic information with the treatment of CRPC.

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Comparison of protein expression in two prostate cancer cell-lines, LNCaP and DU145, after treatment with somatostatin

Cited by 9 publications

References 22 publications

The effect of selenium enrichment on baker's yeast proteome

The effect of selenium enrichment on baker's yeast proteome

Sequenced Combinations of Cisplatin and Selected Phytochemicals Towards Overcoming Drug Resistance in Ovarian Tumour Models

Somatostatin Derivative (smsDX) Targets Cellular Metabolism in Prostate Cancer Cells after Androgen Deprivation Therapy

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