Comparison of protein expression profile of limb regeneration between neotenic and metamorphic axolotl

Sibai, Mustafa; Altuntaş, Ebru; Süzek, Barış E.; Sahin, Betul; Parlayan, Cüneyd; Öztürk, Gürkan; Baykal, Ahmet Tarık; Demircan, Turan

doi:10.1016/j.bbrc.2019.11.118

Cited by 23 publications

(37 citation statements)

References 24 publications

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“…89 However, the complement system has not been fully characterized or analyzed at the genetic level in salamanders, though expression has been observed during limb and lens regeneration processes. [20][21][22][23] During limb regeneration, C3 is expressed in the axolotl and the newt N viridescens, 21 , while C3 and C5 are up-regulated both during limb and lens regeneration in N viridescens. 22 .…”

Section: The Complement Systemmentioning

confidence: 99%

“…Furthermore, using proteomics, the plasma protease C1 inhibitor (C1INH) and complement receptor 4 (CR4) were shown to be differentially expressed during blastema formation in neotenic animals. 23 C1INH, which is up-regulated at 7dpa, is regarded as an anti-inflammatory complement regulator which also interacts with ECM components. 90 In contrast, CR4 is down-regulated, while the rest of the complement system components remain unchanged.…”

Section: The Complement Systemmentioning

confidence: 99%

“…Analysis of the zebrafish genome has shown high conservation of complement system components from mammals 89 . However, the complement system has not been fully characterized or analyzed at the genetic level in salamanders, though expression has been observed during limb and lens regeneration processes 20‐23 …”

Section: Innate Immune Cells In Regenerationmentioning

confidence: 99%

“…Similarly, during heart regeneration, recruitment of macrophages and upregulation of complement system components have been observed 7,20 . In addition, during salamander limb and lens regeneration, complement components are differentially regulated upon tissue injury 21‐23 . Strikingly, upon pharmacological macrophage depletion, limb, and heart regeneration are prevented following injury in the axolotl, suggesting a critical role for immunity in regeneration 7,17 .…”

Section: Introductionmentioning

confidence: 99%

“…7,20 In addition, during salamander limb and lens regeneration, complement components are differentially regulated upon tissue injury. [21][22][23] Strikingly, upon pharmacological macrophage depletion, limb, and heart regeneration are prevented following injury in the axolotl, suggesting a critical role for immunity in regeneration. 7,17 However, the roles of immune system components in salamander regeneration are yet to be fully dissected.…”

Section: Introductionmentioning

confidence: 99%

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Immunity in salamander regeneration: Where are we standing and where are we headed?

Bolaños-Castro

Walters

Vazquez

et al. 2020

Developmental Dynamics

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Salamanders exhibit the most extensive regenerative repertoire among vertebrates, being able to accomplish scar-free healing and faithful regeneration of significant parts of the eye, heart, brain, spinal cord, jaws and gills, as well as entire appendages throughout life. The cellular and molecular mechanisms underlying salamander regeneration are currently under extensive examination, with the hope of identifying the key drivers in each context, understanding interspecies differences in regenerative capacity, and harnessing this knowledge in therapeutic settings. The immune system has recently emerged as a potentially critical player in regenerative responses. Components of both innate and adaptive immunity have been found at critical stages of regeneration in a range of salamander tissues. Moreover, functional studies have identified a requirement for macrophages during heart and limb regeneration. However, our knowledge of salamander immunity remains scarce, and a thorough definition of the precise roles played by its members is lacking. Here, we examine the evidence supporting roles for immunity in various salamander regeneration models. We pinpoint observations that need revisiting through modern genetic approaches, uncover knowledge gaps, and highlight insights from various model organisms that could guide future explorations toward an understanding of the functions of immunity in regeneration.

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Section: The Complement Systemmentioning

confidence: 99%

Section: The Complement Systemmentioning

confidence: 99%

Section: Innate Immune Cells In Regenerationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Immunity in salamander regeneration: Where are we standing and where are we headed?

Bolaños-Castro

Walters

Vazquez

et al. 2020

Developmental Dynamics

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Finding Solutions for Fibrosis: Understanding the Innate Mechanisms Used by Super‐Regenerator Vertebrates to Combat Scarring

Durant

Whited

2021

Advanced Science

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Soft tissue fibrosis and cutaneous scarring represent massive clinical burdens to millions of patients per year and the therapeutic options available are currently quite limited. Despite what is known about the process of fibrosis in mammals, novel approaches for combating fibrosis and scarring are necessary. It is hypothesized that scarring has evolved as a solution to maximize healing speed to reduce fluid loss and infection. This hypothesis, however, is complicated by regenerative animals, which have arguably the most remarkable healing abilities and are capable of scar‐free healing. This review explores the differences observed between adult mammalian healing that typically results in fibrosis versus healing in regenerative animals that heal scarlessly. Each stage of wound healing is surveyed in depth from the perspective of many regenerative and fibrotic healers so as to identify the most important molecular and physiological variances along the way to disparate injury repair outcomes. Understanding how these powerful model systems accomplish the feat of scar‐free healing may provide critical therapeutic approaches to the treatment or prevention of fibrosis.

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TheTale‐TellHeart: Evolutionary tetrapod shift from aquatic to terrestrial life‐style reflected in heart changes in axolotl (Ambystoma mexicanum)

Olejníčková

Kolesová

Bartoš

et al. 2021

Developmental Dynamics

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Background During amphibian metamorphosis, the crucial moment lies in the rearrangement of the heart, reflecting the changes in circulatory demands. However, little is known about the exact shifts linked with this rearrangement. Here, we demonstrate such myocardial changes in axolotl (Ambystoma mexicanum) from the morphological and physiological point of view. Results Micro‐CT and histological analysis showed changes in ventricular trabeculae organization, completion of the atrial septum and its connection to the atrioventricular valve. Based on Myosin Heavy Chain and Smooth Muscle Actin expression we distinguished metamorphosis‐induced changes in myocardial differentiation at the ventricular trabeculae and atrioventricular canal. Using optical mapping, faster speed of conduction through the atrioventricular canal was demonstrated in metamorphic animals. No differences between the groups were observed in the heart rates, ventricular activation times, and activation patterns. Conclusions Transition from aquatic to terrestrial life‐style is reflected in the heart morphology and function. Rebuilding of the axolotl heart during metamorphosis was connected with reorganization of ventricular trabeculae, completion of the atrial septum and its connection to the atrioventricular valve, and acceleration of AV conduction.

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Comparison of protein expression profile of limb regeneration between neotenic and metamorphic axolotl

Cited by 23 publications

References 24 publications

Immunity in salamander regeneration: Where are we standing and where are we headed?

Immunity in salamander regeneration: Where are we standing and where are we headed?

Finding Solutions for Fibrosis: Understanding the Innate Mechanisms Used by Super‐Regenerator Vertebrates to Combat Scarring

TheTale‐TellHeart: Evolutionary tetrapod shift from aquatic to terrestrial life‐style reflected in heart changes in axolotl (Ambystoma mexicanum)

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