Comparison of real-time PCR and Kato smear microscopy for the detection of hookworm infections in three consecutive faecal samples from schoolchildren in Ghana

Mens, Suzan P. van; Aryeetey, Yvonne; Yazdanbakhsh, Maria; Lieshout, Lisette van; Boakye, Daniel; Verweij, Jaco J.

doi:10.1093/trstmh/trs094

Cited by 26 publications

(23 citation statements)

References 4 publications

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“…This finding is in stark contrast to the consistent finding by other molecular-based prevalence studies in the region that N. americanus is the predominant hookworm species in humans, followed by A. ceylanicum and A. duodenale hookworms ( 3 , 5 , 6 , 10 ). PCR proved a superior alternative to microscopy-based techniques for the detection of hookworms in fecal samples ( 25 , 26 ). In Dong village, the prevalence of A. ceylanicum hookworms matched that of their anthroponotic counterpart, N. americanus hookworms, and infections with A. ceylanicum hookworms substantially out-numbered those with A. duodenale hookworms.…”

Section: Discussionmentioning

confidence: 99%

High Prevalence ofAncylostoma ceylanicumHookworm Infections in Humans, Cambodia, 2012

Inpankaew¹,

Schär²,

Dalsgaard³

et al. 2014

Emerg. Infect. Dis.

135

156

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Section: Discussionmentioning

confidence: 99%

High Prevalence ofAncylostoma ceylanicumHookworm Infections in Humans, Cambodia, 2012

Inpankaew¹,

Schär²,

Dalsgaard³

et al. 2014

Emerg. Infect. Dis.

135

156

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“…To our knowledge, this is the first study applying PCR-based diagnosis of A. lumbricoides to preventive chemotherapy. PCR assays have previously shown superior sensitivity in the detection of STHs (Basuni et al 2011;Jonker et al 2012;van Mens et al 2013). In the present study, one-third of the initial A. lumbricoides infections and of those detected after 3 months were diagnosed by PCR exclusively as were seven of the eight infections 2 weeks following treatment.…”

Section: Discussionmentioning

confidence: 99%

Soil‐transmitted helminths in southern highland Rwanda: associated factors and effectiveness of school‐based preventive chemotherapy

Staudacher

Heimer

Steiner

et al. 2014

Tropical Med Int Health

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Abstractobjectives Preventive chemotherapy of schoolchildren against soil-transmitted helminths (STHs) is widely implemented in Rwanda. However, data on its actual efficacy are lacking. We assessed prevalence, associated factors and manifestation of STH infection among schoolchildren in southern highland Rwanda as well as cure and reinfection rates.methods Six hundred and twenty-two children (rural, 301; urban, 321) were included preceding the administration of a single dose of 500 mg mebendazole. Before treatment, and after 2 and 15 weeks, STH infection was determined by Kato-Katz smears and by PCR assays for Ascaris lumbricoides. Clinical and anthropometric data, socio-economic status and factors potentially associated with STH infection were assessed.results Soil-transmitted helminth (STH) infection was present in 38% of rural and in 13% of urban schoolchildren. Ascaris lumbricoides accounted for 96% of infections. Of these, one-third was detected by PCR exclusively. Factors associated with STH infection differed greatly between rural and urban children. Likewise, STH infection was associated with stunting and anaemia only among urban children. The cure rate after 2 weeks was 92%. Among eight non-cleared A. lumbricoides infections, seven were submicroscopic. Reinfection within 3 months occurred in 7%, but the rate was higher among rural children, and with initially present infection, particularly at comparatively high intensity.conclusions The rural-urban difference in factors associated with STH infection and in reinfection rates highlights the need for targeted interventions to reduce transmission. PCR assays may help in detecting low-level infections persisting after treatment. In southern Rwanda, mebendazole is highly effective against the STH infections predominated by A. lumbricoides.

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“…In an area of low N. americanus endemicity in southern Ghana, the detection rate by PCR for a single fecal sample almost equaled that of Kato examination of three fecal samples. Additionally, N. americanus infections were detected in 8 of 31 microscopy-negative cases by PCR using three consecutive fecal samples (329). The same primer-and-probe design from the ITS2 sequence was used in multiplex TaqMan-based assays targeting different helminth infections, in singleplex assays tested in parallel with other targets, and in a multiplex PCR combined with probe-based detection with Luminex beads (Table 7).…”

Section: Hookwormmentioning

confidence: 99%

Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections

2014

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SUMMARY Over the past few decades, nucleic acid-based methods have been developed for the diagnosis of intestinal parasitic infections. Advantages of nucleic acid-based methods are numerous; typically, these include increased sensitivity and specificity and simpler standardization of diagnostic procedures. DNA samples can also be stored and used for genetic characterization and molecular typing, providing a valuable tool for surveys and surveillance studies. A variety of technologies have been applied, and some specific and general pitfalls and limitations have been identified. This review provides an overview of the multitude of methods that have been reported for the detection of intestinal parasites and offers some guidance in applying these methods in the clinical laboratory and in epidemiological studies.

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Comparison of real-time PCR and Kato smear microscopy for the detection of hookworm infections in three consecutive faecal samples from schoolchildren in Ghana

Abstract: PCR provides a sensitive alternative to the conventional microscopic detection of hookworm infections.

Cited by 26 publications

References 4 publications

High Prevalence ofAncylostoma ceylanicumHookworm Infections in Humans, Cambodia, 2012

High Prevalence ofAncylostoma ceylanicumHookworm Infections in Humans, Cambodia, 2012

Soil‐transmitted helminths in southern highland Rwanda: associated factors and effectiveness of school‐based preventive chemotherapy

Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections

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