Comparison of RNA content from hydrophobic interaction chromatography‐isolated seminal plasma exosomes from intrauterine insemination (IUI) pregnancies

Abstract

About 50% of infertility cases involve male factors (Winters & Walsh, 2014). Over the past several decades, there has been a reported decline in sperm count worldwide (Levine et al., 2017;Sengupta et al., 2018). The current leading diagnosis for male infertility is thought to be idiopathic, with falling sperm counts and semen quality thought to stem from factors such as obesity, illicit substance use, smoking, excessive alcohol use or environmental toxin exposure (Barazani et al., 2014;Krzastek et al., 2020). Furthermore, many different explanatory hypotheses have been developed delineating potential mechanisms contributing to male-factor infertility, including

Isolation and usage of exosomes in central nervous system diseases

“…Chemical separation and processing platforms, similar to those used in high performance liquid chromatography (HPLC), present a potential alternative approach. Hydrophobic interaction chromatography (HIC) can be employed to extract exosomes from seminal plasma obtained through intrauterine insemination (IUI) treatments 46 . In 2019, Huang et al.…”

“…Hydrophobic interaction chromatography (HIC) can be employed to extract exosomes from seminal plasma obtained through intrauterine insemination (IUI) treatments. 46 In 2019, Huang et al developed a protocol for the isolation and quantification of human urinary exosomes using HIC on a polyester (PET) capillary-channeled polymer (C-CP) fiber stationary phase. 87 Wang and colleagues investigated the application of PET C-CP fibers in an HIC protocol for extracting exosomes from a human plasma sample.…”

Rapid isolation and quantification of extracellular vesicles from suspension‐adapted human embryonic kidney cells using capillary‐channeled polymer fiber spin‐down tips

“…Marcus and colleagues have developed a hydrophobicity‐based EV isolation method employing capillary‐channeled polymer (C‐CP) fiber stationary phases to address the shortcomings of the currently available EV isolation methods [ 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. These C‐CP fiber phases have been employed in highly efficient EV isolations via high‐performance liquid chromatography (HPLC) [ 32 – 34 , 38 , 39 ] and solid‐phase extraction (SPE) tip [ 35 – 37 , 40 ] formats, concentrating on what would be called analytical‐scale processing.…”

“…Marcus and colleagues have developed a hydrophobicity‐based EV isolation method employing capillary‐channeled polymer (C‐CP) fiber stationary phases to address the shortcomings of the currently available EV isolation methods [ 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. These C‐CP fiber phases have been employed in highly efficient EV isolations via high‐performance liquid chromatography (HPLC) [ 32 – 34 , 38 , 39 ] and solid‐phase extraction (SPE) tip [ 35 – 37 , 40 ] formats, concentrating on what would be called analytical‐scale processing. In both cases, the isolation of EVs is driven by an organic modifier‐assisted hydrophobic interaction chromatography (HIC) solvent system, where EVs have been obtained from several complex biofluids, including urine, saliva, blood serum, cervical mucus, and CCM from Dictyostelium discoideum cell culture [ 32 , 35 , 39 ].…”

“…In previous C‐CP‐based HIC isolations of EVs, acetonitrile (ACN) and glycerol solvent additives were utilized to aid the elution of the vesicles from the fiber surface [ 32 , 39 ]. The ACN solvent additive was proven most compatible for EVs subsequently analyzed by MS, RNA sequencing, and immune characterization approaches [ 34 , 38 ]. Nevertheless, the high concentrations of ACN are not ideal for the long‐term stability of EVs, though most of the latent ACN can be removed using a simple off‐gassing process under a low vacuum.…”