Comparison of Salmonella enterica serovar Bovismorbificans 2011 hummus outbreak strains with non-outbreak strains

Blaylock, Morris; Blackwell, Reginald; Merid, Sosina; Jackson, Scott A.; Kotewicz, Michael L.; Gopinath, Gopal; Ayers, Sherry; Abbott, Jason; Sabo, Jonathan L.; Ewing, Laura; Gangiredla, Jayanthi; Gebru, Solomon T.; Patel, Isha R.; Jones, Brooke; Dudley, Kim; Jarvis, Karen G.; Hanes, Darcy E.; Diallo, Alpha; Beaubrun, Junia Jean-Gilles

doi:10.1016/j.fm.2014.02.016

Cited by 6 publications

(5 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Typhimurium circulate among the studied raccoon population. S. Bovismorbificans is a serovar that has been previously associated with outbreaks in humans, especially in developed countries (Blaylock et al, ; Gilsdorf et al, ; Hendriksen et al, ; Mattila et al, ). It can colonize and persist in different species of animals and may contaminate meat products for human consumption (Nógrády, Imre, Kostyák, Tóth, & Nagy, ).…”

Section: Discussionmentioning

confidence: 99%

“…S. Bovismorbificans is a serovar that has been previously associated with outbreaks in humans, especially in developed countries (Blaylock et al, 2015;Gilsdorf et al, 2005;Hendriksen et al, 2011;Mattila et al, 1998). It can colonize and persist in different species of animals and may contaminate meat products for human consumption (Nógrády, Imre, Kostyák, Tóth, & Nagy, 2010 raccoons were similar to those recovered from clinically ill patients in Pennsylvania, USA.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Salmonellosis detection and evidence of antibiotic resistance in an urban raccoon population in a highly populated area, Costa Rica

Baldi

Barquero-Calvo

Hutter

et al. 2019

Zoonoses and Public Health

View full text Add to dashboard Cite

Wild animals are involved in zoonotic disease transmission cycles. These are generally complex and poorly understood, especially among animals adapted to life in human ecosystems. Raccoons are reservoirs and effective carriers for infectious agents such as Salmonella throughout different environments and contribute to the transference of resistance genes. This study examined the presence of circulating Salmonella sp. in a population of raccoons in a tropical urban environment and evaluated resistance to antibiotics commonly used to treat salmonellosis. A total of 97 raccoons of different ages and sex were included in this study. 49% (38–60 CI) of the faecal samples were positive for Salmonella spp. The study identified 15 circulating serovars with the most prevalent being S. Hartford (7/15), S. Typhimurium (4/15) and S. Bovismorbificans (4/15). These serovars correspond to the serovars detected in humans with clinical symptoms in Costa Rica. 9.5% of the Salmonella strains recovered demonstrated ciprofloxacin resistance, and 7.1% showed resistance to nalidixic acid. This study provides evidence of multiple Salmonella serovars circulating in a population of urban raccoons in Costa Rica. Furthermore, the study confirms the existence of antimicrobial resistance to two antibiotics used to treat human salmonellosis. The findings emphasize the role of the raccoon as a reservoir of Salmonella in the Greater Metropolitan Area of Costa Rica (GAM) and stress the need for active monitoring of the presence and possible spread in antibiotic resistance due to this peri‐domestic carnivore.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Salmonellosis detection and evidence of antibiotic resistance in an urban raccoon population in a highly populated area, Costa Rica

Baldi

Barquero-Calvo

Hutter

et al. 2019

Zoonoses and Public Health

View full text Add to dashboard Cite

show abstract

“…Plas-midFinder web tool identified IncFIB(s), InfFII(s) plasmids in 59 out of 60 Cluster 1 strains (Supplemental Figure S3), while other plasmid types such as IncI1, IncI2, and colI56 were noted in a few strains (Supplemental File S5). Previously, the presence of a putative 90 kb plasmid was predicted [7] in some of the hummus-outbreak strains included in this study (Table 1). We designed a local BLAST analysis to identify the homologous sequences of this plasmid in the WGS assemblies.…”

Section: Plasmids Phage Amr and Virulence Profiling Of S Bovismorbifi...mentioning

confidence: 80%

“…S. Bovismorbificans strains collected from Switzerland, the USA, and Canada were provided by University of Zurich, University of Wisconsin-Madison, and Health Canada, respectively. In addition to the newly sequenced 81 strains, genomes from 14 S. Bovismorbificans genomes, which were associated with several hummus USA outbreaks, were included [7,11,14]. For comparative analysis, a scaffolded genome from the clinical strain (3114) from Malawi [9], an environmental strain (CIES13) from Mexico, and 25 more S. Bovismorbificans genomes were downloaded from NCBI.…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…The molecular epidemiology of a sprout-borne outbreak of S. Bovismorbificans [4,5] in Finland had been reported earlier. Most foodborne outbreaks associated with S. Bovismorbificans reported in Europe, the USA, and Canada has been traced to pork products, lettuce, hummus, and sprouts [6][7][8]. The first genomes of S. Bovismorbificans were generated from a Malawian bacteremia case and UK veterinary samples belonging to Sequence Type (ST)142 (ST142) including that of a virulence plasmid pVirBov from clinical strain 3114 [9].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenomic Analysis of Salmonella enterica subsp. enterica Serovar Bovismorbificans from Clinical and Food Samples Using Whole Genome Wide Core Genes and kmer Binning Methods to Identify Two Distinct Polyphyletic Genome Pathotypes

et al. 2022

Self Cite

View full text Add to dashboard Cite

Salmonella enterica subsp. enterica serovar Bovismorbificans has caused multiple outbreaks involving the consumption of produce, hummus, and processed meat products worldwide. To elucidate the intra-serovar genomic structure of S. Bovismorbificans, a core-genome analysis with 2690 loci (based on 150 complete genomes representing Salmonella enterica serovars developed as part of this study) and a k-mer-binning based strategy were carried out on 95 whole genome sequencing (WGS) assemblies from Swiss, Canadian, and USA collections of S. Bovismorbificans strains from foodborne infections. Data mining of a digital DNA tiling array of legacy SARA and SARB strains was conducted to identify near-neighbors of S. Bovismorbificans. The core genome analysis and the k-mer-binning methods identified two polyphyletic clusters, each with emerging evolutionary properties. Four STs (2640, 142, 1499, and 377), which constituted the majority of the publicly available WGS datasets from >260 strains analyzed by k-mer-binning based strategy, contained a conserved core genome backbone with a different evolutionary lineage as compared to strains comprising the other cluster (ST150). In addition, the assortment of genotypic features contributing to pathogenesis and persistence, such as antimicrobial resistance, prophage, plasmid, and virulence factor genes, were assessed to understand the emerging characteristics of this serovar that are relevant clinically and for food safety concerns. The phylogenomic profiling of polyphyletic S. Bovismorbificans in this study corresponds to intra-serovar variations observed in S. Napoli and S. Newport serovars using similar high-resolution genomic profiling approaches and contributes to the understanding of the evolution and sequence divergence of foodborne Salmonellae. These intra-serovar differences may have to be thoroughly understood for the accurate classification of foodborne Salmonella strains needed for the uniform development of future food safety mitigation strategies.

show abstract

First report of two foodborne Salmonella enterica subsp. enterica serovar Bovismorbificans isolates carrying a novel mega-plasmid harboring blaDHA-1 and qnrB4 genes

Olsen

Wang

et al. 2021

International Journal of Food Microbiology

View full text Add to dashboard Cite

Comparison of Salmonella enterica serovar Bovismorbificans 2011 hummus outbreak strains with non-outbreak strains

Cited by 6 publications

References 14 publications

Salmonellosis detection and evidence of antibiotic resistance in an urban raccoon population in a highly populated area, Costa Rica

Salmonellosis detection and evidence of antibiotic resistance in an urban raccoon population in a highly populated area, Costa Rica

Phylogenomic Analysis of Salmonella enterica subsp. enterica Serovar Bovismorbificans from Clinical and Food Samples Using Whole Genome Wide Core Genes and kmer Binning Methods to Identify Two Distinct Polyphyletic Genome Pathotypes

First report of two foodborne Salmonella enterica subsp. enterica serovar Bovismorbificans isolates carrying a novel mega-plasmid harboring blaDHA-1 and qnrB4 genes

Contact Info

Product

Resources

About