Comparison of Sensitivity between Yellowtail Seriola quinqueradiata and Red Sea Bream Pagrus major to Nocardia seriolae

公一, 板野; 典義, 中岡; 秀昌, 川上; 智也, 河野; 正博, 酒井

doi:10.3147/jsfp.41.135

Cited by 11 publications

(9 citation statements)

References 10 publications

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“…Like causal agents of human nocardiosis, N . seriolae is considered to be an intracellular pathogen that invades and grows within host cells, even including phagocytes [ 44 , 45 ]. The intracellular nature of this bacteria makes it difficult to control disease.…”

Section: Resultsmentioning

confidence: 99%

Analysis of the complete genome sequence of Nocardia seriolae UTF1, the causative agent of fish nocardiosis: The first reference genome sequence of the fish pathogenic Nocardia species

et al. 2017

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Nocardiosis caused by Nocardia seriolae is one of the major threats in the aquaculture of Seriola species (yellowtail; S. quinqueradiata, amberjack; S. dumerili and kingfish; S. lalandi) in Japan. Here, we report the complete nucleotide genome sequence of N. seriolae UTF1, isolated from a cultured yellowtail. The genome is a circular chromosome of 8,121,733 bp with a G+C content of 68.1% that encodes 7,697 predicted proteins. In the N. seriolae UTF1 predicted genes, we found orthologs of virulence factors of pathogenic mycobacteria and human clinical Nocardia isolates involved in host cell invasion, modulation of phagocyte function and survival inside the macrophages. The virulence factor candidates provide an essential basis for understanding their pathogenic mechanisms at the molecular level by the fish nocardiosis research community in future studies. We also found many potential antibiotic resistance genes on the N. seriolae UTF1 chromosome. Comparative analysis with the four existing complete genomes, N. farcinica IFM 10152, N. brasiliensis HUJEG-1 and N. cyriacigeorgica GUH-2 and N. nova SH22a, revealed that 2,745 orthologous genes were present in all five Nocardia genomes (core genes) and 1,982 genes were unique to N. seriolae UTF1. In particular, the N. seriolae UTF1 genome contains a greater number of mobile elements and genes of unknown function that comprise the differences in structure and gene content from the other Nocardia genomes. In addition, a lot of the N. seriolae UTF1-specific genes were assigned to the ABC transport system. Because of limited resources in ocean environments, these N. seriolae UTF1 specific ABC transporters might facilitate adaptation strategies essential for marine environment survival. Thus, the availability of the complete N. seriolae UTF1 genome sequence will provide a valuable resource for comparative genomic studies of N. seriolae isolates, as well as provide new insights into the ecological and functional diversity of the genus Nocardia.

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Section: Resultsmentioning

confidence: 99%

Analysis of the complete genome sequence of Nocardia seriolae UTF1, the causative agent of fish nocardiosis: The first reference genome sequence of the fish pathogenic Nocardia species

et al. 2017

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“…In this study, we aimed to evaluate effects of skin injuries on the vulnerability of Japanese amberjack to Nocardia seriolae infection by immersion. Itano et al (2006) calculated the LD 50 value of N. seriolae of Japanese amberjack (60.8 g in mean body weight) as 1.2 × 10 4 CFU/mL by immersion. Based on this, we challenged injured Japanese amberjack at 1.0 × 10 4 CFU/mL, in which mortality was higher than expected and no difference was observed between the injured and uninjured fish.…”

Section: Discussionmentioning

confidence: 99%

Skin Injuries Contribute to Nocardiosis in Japanese Amberjack <i>Seriola</i> <i>quinqueradiata</i>

Miyoshi¹,

Fukuda

Ogawa

2019

Fish Pathol.

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Nocardia seriolae, a Gram-positive, weakly acidfast, filamentous bacterium, is the causative agent of nocardiosis of cultured amberjacks Seriola spp. in Japan (Kudo et al., 1988). This infectious disease was first noticed in ca. 1967 (Kariya et al., 1968) and is still causing economic damages to the aquaculture industry. N. seriolae is sensitive to several antimicrobial agents in vitro (Itano and Kawakami, 2002), which, however, show a low killing effect in vivo on the bacterium in the nodules formed in the viscera of infected fish. Currently, several sulfonamides like sulfamonomethoxine are approved to be used as therapeutic agents of nocardiosis, but it is practically difficult to diagnose fish in an early stage of infection before the nodule formation (Itano et al., 2008). Development of vaccines against nocardiosis is

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“…Itano et al [24] previously suggested that the superoxide anion from phagocytes is an important factor in protecting against nocardiosis. Superoxide anion, H 2 O 2 , and the hydroxyl free radical are reactive oxygen species (ROSs), which are highly bactericidal.…”

Section: Discussionmentioning

confidence: 99%

“…Notably, N. asteroides is resistant to ROSs such as H 2 O 2 due to the production of superoxide dismutase and catalase, which are found in virulent strains [29][30][31]. A previous study reported that N. seriolae cells were engulfed by yellowtail phagocyte cells [24]. However, whether N. seriolae can survive or grow within phagocyte cells remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Immune response of largemouth bass, Micropterus salmoides, to whole cells of different Nocardia seriolae strains

et al. 2010

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Largemouth bass Micropterus salmoides were immunized with four different N. seriolae strains-two a-glucosidase-positive (961113, KU040801) and two a-glucosidase-negative (94260, OTTS) strains-along with Freund's incomplete adjuvant. After primary immunization (week 0), a booster was administered at weeks 4 and 8. Nonspecific immune responses to multiple immunizations with the different N. seriolae strains were determined based on serum lysozyme activity and nitroblue tetrazolium (NBT)-positive cells in peripheral blood. The serum lysozyme activity and NBT-positive cells in peripheral blood were not significantly increased even after the two booster immunizations. Specific antibody responses against N. seriolae cells were investigated by enzyme-linked immunosorbent assay. At 4 weeks after immunization, all groups immunized with N. seriolae antigens showed significant increases in their specific antibody levels. The sera from fish immunized with different N. seriolae strains exhibited reactivity with N. seriolae sonicated antigens of 28, 30, 36 and 84 kDa by western blot analysis. After two boosters, fish were challenged with live N. seriolae to assess the vaccine's efficacy; however, multiple injections of the N. seriolae strains did not reduce mortality, irrespective of the bacterin.

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Comparison of Sensitivity between Yellowtail Seriola quinqueradiata and Red Sea Bream Pagrus major to Nocardia seriolae

Cited by 11 publications

References 10 publications

Analysis of the complete genome sequence of Nocardia seriolae UTF1, the causative agent of fish nocardiosis: The first reference genome sequence of the fish pathogenic Nocardia species

Analysis of the complete genome sequence of Nocardia seriolae UTF1, the causative agent of fish nocardiosis: The first reference genome sequence of the fish pathogenic Nocardia species

Skin Injuries Contribute to Nocardiosis in Japanese Amberjack <i>Seriola</i> <i>quinqueradiata</i>

Immune response of largemouth bass, Micropterus salmoides, to whole cells of different Nocardia seriolae strains

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