Comparison of sensitizers by detecting reactive oxygen species after photodynamic reaction in vitro

Can Photodynamic Therapy Be an Alternative Method in Melanoma Treatment?

“…The application of comet assay to study the influence of Ph-PDT on BM cells showed that some types of DNA damage depend on photosensitizer concentration, as well as on time and dose of irradiation. Similar results were obtained by Barge et al (Barge et al, 2004) in human melanoma cells using a new silicon phthalocyanine photosensitizer and by Haddad et al in murine malignant melanoma in vitro and in vivo using aluminum phthalocyanine (AlpcS4) (Haddad et al, 1998) Higher doses of light and sensitizer lead to a dose-dependent augmentation of reaction oxygen species in melanoma cells (Kästle et al, 2011;Kolarova et al, 2007). A widely used photosensitizer is the protoporphyrin IX (PpIX) precursor 5-aminolevulinic acid (ALA).…”

“…demonstrate that the photodynamic effect depends on sensitizer type, its concentration and light dose (Kolarova et al, 2007). Later research showed the most significant phototoxic effect of chloraluminium phthalocyanine disulfonate (ClAlPcS 2 )-PDT in spite of significantly higher ROS production induced by ZnTPPS 4 -PDT on G361 cells (Krestyn et al, 2010).…”

Interaction of Wheat Germ Agglutinin with Porphyrin Compounds—Potential Anticancer Agents

“…Interestingly, Kolarova et al (19) found that PdTPPS 4 is effective in producing ROS, which is important for the outcome in PDT, and in induction of cell death in G361 (a human melanoma cell line). In PDT, porphyrins selectively accumulate in the target cancer cells and, after irradiation with visible light, produce singlet oxygen, generating ROS that causes cell death.…”

“…Recently, a remarkable antiproliferative effect of Fe and Pd on cancer cells is reported (19,25) which urged us to study WGA-Fe and WGA-Pd porphyrin complexes opening new possibilities for the targeted drug delivery for cancer.…”

A novel visible light responsive nanosystem for cancer treatment

“…22,23 However It has been observed that this energy is already enough to activate some photosensitizers in the production of reactive oxygen species (ROS). 24,25 ROS are highly reactive, especially with organic compounds that contain double bonds. It has been reported the spontaneous cleavage of aminoacrylates (β-enamino esters) following 2+2 cycloaddition reactions of singlet oxygen with olefins for the site-specific release of bioactive molecules.…”