Comparison of serum C3 complement levels between young women with recurrent urinary tract infection and healthy women

Syukri, Maimun; Imran, Imran; Harapan, Harapan; Sja’bani, Mochammad; Astuti, Indwiani; Soesatyo, Marsetyawan Hne

doi:10.1016/j.ajme.2014.04.006

Cited by 8 publications

(8 citation statements)

References 30 publications

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“…Interestingly, decreased APP expression has been associated with recurrent bacterial infections. For example, C3 levels in women with recurrent urinary tract infections have been shown to be lower than in healthy women ( Syukri et al., 2015 ). In addition, mutations that cause complement deficiencies predispose individuals to recurrent infections (e.g., by Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenza e) ( Ram et al., 2010 ).…”

Section: Discussionmentioning

confidence: 99%

An Immunomodulatory Transcriptional Signature Associated With Persistent Listeria Infection in Hepatocytes

Descoeudres¹,

Jouneau²,

Henry³

et al. 2021

Front. Cell. Infect. Microbiol.

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Listeria monocytogenes causes severe foodborne illness in pregnant women and immunocompromised individuals. After the intestinal phase of infection, the liver plays a central role in the clearance of this pathogen through its important functions in immunity. However, recent evidence suggests that during long-term infection of hepatocytes, a subpopulation of Listeria may escape eradication by entering a persistence phase in intracellular vacuoles. Here, we examine whether this long-term infection alters hepatocyte defense pathways, which may be instrumental for bacterial persistence. We first optimized cell models of persistent infection in human hepatocyte cell lines HepG2 and Huh7 and primary mouse hepatocytes (PMH). In these cells, Listeria efficiently entered the persistence phase after three days of infection, while inducing a potent interferon response, of type I in PMH and type III in HepG2, while Huh7 remained unresponsive. RNA-sequencing analysis identified a common signature of long-term Listeria infection characterized by the overexpression of a set of genes involved in antiviral immunity and the under-expression of many acute phase protein (APP) genes, particularly involved in the complement and coagulation systems. Infection also altered the expression of cholesterol metabolism-associated genes in HepG2 and Huh7 cells. The decrease in APP transcripts was correlated with lower protein abundance in the secretome of infected cells, as shown by proteomics, and also occurred in the presence of APP inducers (IL-6 or IL-1β). Collectively, these results reveal that long-term infection with Listeria profoundly deregulates the innate immune functions of hepatocytes, which could generate an environment favorable to the establishment of persistent infection.

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Section: Discussionmentioning

confidence: 99%

An Immunomodulatory Transcriptional Signature Associated With Persistent Listeria Infection in Hepatocytes

Descoeudres¹,

Jouneau²,

Henry³

et al. 2021

Front. Cell. Infect. Microbiol.

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“…Deficient C3 may result in ineffective bacterial clearance, thus resulting in severe infection. 30 31 Syukri et al 31 noticed that serum C3 levels were significantly lower in patients with recurrent UTIs than in healthy younger women (mean 42.08 µg/mL ±1.20 vs 42.75 µg/mL ±0.71, p=0.008). However, Springall et al 32 used a mouse model to demonstrate conflicting results in which C3 deficiency prevented an ascending renal infection.…”

Section: Discussionmentioning

confidence: 98%

Vaginal microbiota affects urinary tract infection risk in women with systemic lupus erythematosus: a pilot cross-sectional study from Thailand

et al. 2021

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ObjectiveThe risk factors associated with urinary tract infections (UTIs) in patients with SLE remain uncertain. We evaluated the vaginal microbiota pattern and its potential UTI-associated risk factors.MethodsA pilot cross-sectional study of patients with SLE was conducted at Ramathibodi Hospital, Bangkok, Thailand, during 2019–2020. Patients’ demographic data and relevant information were collected. Vaginal microbiota was assessed in all patients and in 10 healthy volunteers.ResultsFifty-two patients were enrolled (mean age: 46.1 years). All patients had SLE that was in low disease activity. As per the Simpson_e index, the within-group alpha diversity of the vaginal microbiota was low in the SLE with UTI and SLE receiving trimethoprim-sulfamethoxazole (TMP-SMX) prophylaxis groups. Multivariate logistic regression analysis revealed that TMP-SMX prophylaxis (adjusted OR (AOR), 30.96; 95% CI 3.63 to 264.11; p=0.002), elevated C3 levels (AOR, 35.33; 95% CI 1.33 to 936.67; p=0.033) and presence of Veillonella dispar in the vaginal microbiota (AOR, 6.68; 95% CI 1.27 to 35.07; p=0.025) were associated with UTI.ConclusionsThe vaginal microbiota diversity differed between patients with lupus with and without UTI, and unnecessary administration of TMP-SMX prophylaxis may affect the alpha diversity of the vaginal microbiota.

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“…These results agreed with that reported by others (68) and disagree with the results of (70). Several studies have been conducted to assess the role of C3 in infectious diseases (70)(71)(72)(73). The increasing of complement component C3 in the blood circulation of patients with UTI may return to the production of complement proteins by hepatocytes and several extrahepatic tissues that involved glomerular epithelial cells, endothelial cells, mesangial cells and human proximal tubular epithelial cells (74,75).…”

Section: Discussionmentioning

confidence: 99%

Serum levels of immunoglobulin and complement in UTI of patients caused by Proteus mirabilis and using AgNPs as antiswarming

Otraqchi

Darogha

Ali

2021

Cell Mol Biol (Noisy-le-grand)

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The use of plant extracts represents a promising approach for the synthesis of silver nanoparticles (AgNPs). This study reports the low-cost, green synthesis of AgNPs using the extract of clove and black seeds. The biosynthesized AgNPs were confirmed and characterized by analysis of the spectroscopy profile of the UV-visible spectrophotometer. The purpose of the present study is to evaluate the inhibitory effect concentration (MIC) of AgNPs, clove, and black cumin seed extracts on the growth and swarming of P. mirabilis. Clinical isolates of P. mirabilis were isolated from patients suffering from urinary tract infections. Thirteen types of antibiotics were used in the present study to detect their ability to inhibit P. mirabilis's resistance. Immunological findings included the determination of serum levels of IgG, IgM, IgA and complement protein C3 and C4. Results showed that IgG and IgA concentrations significantly increased (1311.13 ± 72.54 and 279 ± 21.31) respectively in UTI patients in comparison to the healthy control group which was 1089.88 ± 37.33 and 117.611 ± 4.19 respectively, While IgM concentrations were increased non significantly in UTI patients (153.331 ± 6.45) in comparison to healthy control (145.2 ± 13.49). Complement components C3 showed a significant increase in UTI patients with mean values of 125.95 ± 6.22 compared to the control group with mean values of 55.191 ± 9.64, while C4 showed statically non-significant among UTI patients in comparison with the control group (35.195 ± 2.34 and 34.371 ± 1.22) respectively.

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Comparison of serum C3 complement levels between young women with recurrent urinary tract infection and healthy women

Cited by 8 publications

References 30 publications

An Immunomodulatory Transcriptional Signature Associated With Persistent Listeria Infection in Hepatocytes

An Immunomodulatory Transcriptional Signature Associated With Persistent Listeria Infection in Hepatocytes

Vaginal microbiota affects urinary tract infection risk in women with systemic lupus erythematosus: a pilot cross-sectional study from Thailand

Serum levels of immunoglobulin and complement in UTI of patients caused by Proteus mirabilis and using AgNPs as antiswarming

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