Serum levels of immunoglobulin and complement in UTI of patients caused by Proteus mirabilis and using AgNPs as antiswarming

Abstract

The use of plant extracts represents a promising approach for the synthesis of silver nanoparticles (AgNPs). This study reports the low-cost, green synthesis of AgNPs using the extract of clove and black seeds. The biosynthesized AgNPs were confirmed and characterized by analysis of the spectroscopy profile of the UV-visible spectrophotometer. The purpose of the present study is to evaluate the inhibitory effect concentration (MIC) of AgNPs, clove, and black cumin seed extracts on the growth and swarming of P. mirabilis. Clinical isolates of P. mirabilis were isolated from patients suffering from urinary tract infections. Thirteen types of antibiotics were used in the present study to detect their ability to inhibit P. mirabilis's resistance. Immunological findings included the determination of serum levels of IgG, IgM, IgA and complement protein C3 and C4. Results showed that IgG and IgA concentrations significantly increased (1311.13 ± 72.54 and 279 ± 21.31) respectively in UTI patients in comparison to the healthy control group which was 1089.88 ± 37.33 and 117.611 ± 4.19 respectively, While IgM concentrations were increased non significantly in UTI patients (153.331 ± 6.45) in comparison to healthy control (145.2 ± 13.49). Complement components C3 showed a significant increase in UTI patients with mean values of 125.95 ± 6.22 compared to the control group with mean values of 55.191 ± 9.64, while C4 showed statically non-significant among UTI patients in comparison with the control group (35.195 ± 2.34 and 34.371 ± 1.22) respectively.

Tissue Engineering Material KLD-12 Polypeptide /TGF-β1 the Protective Effect and Mechanism of Nanofiber Gel on Early Intervertebral Disc Degeneration

“…Based on the research on nanomaterials particularly nanofiber gel, they have made some progress (17)(18)(19). Nakazawa K R and Duran S research indicate that the normal nucleus pulposus tissue itself is a highly hydrated gelatin viscoelastic tissue with a water content of 70%-85%.…”

Controlling Pepper Mild Mottle Virus (PMMoV) Infection in Pepper Seedlings by Use of Chemically Synthetic Silver Nanoparticles

“…The use of safer and more environmentally friendly nanoparticle synthesis methods, including biological methods using many living organisms such as primitive nuclei (from bacteria and actinomyces) or eukaryotes (fungi and plants), is gaining worldwide attention [15,16]. Synthetic silver nanoparticles (AgNPs) formed by chemical reduction methods have recently been used as antiviral, antibacterial [17][18][19], antifungal [20], and anticancer agents [21].…”