The study was conducted to investigate genetic and environmental factors affecting, and estimating genetic parameters, for milk production and milking-ability traits of Holstein cows. The data obtained from six herds from a commercial farm called Dina for agriculture investment, located in Cairo-Alex. desert road (80 Km) from Cairo, Menoufia, Egypt. It included 3881 cows as progenies of 3321 dams and 261 sires in 6 herds that represented the period from 2007 to 2014. Studied Traits are milk production trait 305-days milk yield (305-dMY), and milking-ability traits like average flow rate (kg/min), peak flow rate (kg/min) and milking duration (min) for cows have Age at First Calving (AFC) equal 24.8±2.3 month. The means (CV%) of milk production and milking-ability traits as 305-dMY, AFR, PF, and MD were 9399±1767 kg, (18.8%), 2.21±0.78 kg/min, (35%), 3.93 ± 1 kg/min, (25%), and 4.09±1.29 min, (32%), respectively. Sire, dam, herd, parity, year, and season of calving had significant effects on studied traits. Heritability estimates were 0.27 ±0.061, 0.20 ± 0.013, 0.07 ±0.002, and 0.09 ±0.001 for 305-dMY, AFR, PF, and MD, respectively. Estimates of rG and rP among studied traits were positive. Moderate estimates of heritability and positive genetic correlation among certain studied traits suggested that genetic improvement would be achieved via selection of appropriate breeding program.