Comparison of sporulation of Paecilomyces farinosus and Beauveria bassiana in batch and fed-batch culture

Humphreys, Anne M.; Matewele, Paul; Cunliffe, Bryan; Trinci, Anthony P. J.

doi:10.1016/s0953-7562(09)81331-2

Cited by 9 publications

(6 citation statements)

References 11 publications

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“…The conidia concentration in PD after 30 h of fermentation to ESALQ1205 was similar to the mean yield of I. farinosa blastospores from 2,500 mL peptone-dextrose liquid medium after four days of culture, which was 4.6 × 10 5 mL -1 (Agudelo and Falcon, 1983). Humphreys et al (1990) showed that the sporulation of I. farinosa in submerged culture decreased when poor carbon sources were used or under limited amount of nitrogen, as observed here for the molasses medium. In another studies, the bean broth medium promoted the highest submerged conidia production for M. anisopliae and B. bassiana (Cruz et al, 1993;Batista Filho et al, 1985).…”

Section: Resultssupporting

confidence: 81%

“…Color changes and medium consistency could be related to the intense growth of fungal mycelia, nutrient metabolism and production of several metabolites. Furthermore, after 24 h of fermentation, all media presented mycelial pellet formation for both fungi, increasing their sizes until the last 94 h. The presence of mycelial pellets in liquid fermentation has already been observed for I. farinosa and I. fumosorosea previously (Humphreys et al, 1990;Torre and Cardenas-Cota, 1996). The maximum production of dry biomass for I. fumosorosea obtained by Torre and CardenasCota, (1996) was 7.5 mg.mL -1 in submerged culture after four days at 37°C with 12:12 (L:D) h photoperiod.…”

Section: Resultssupporting

confidence: 68%

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Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

Mascarin

Alves

Lopes

2010

Braz. arch. biol. technol.

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Section: Resultssupporting

confidence: 81%

Section: Resultssupporting

confidence: 68%

Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

Mascarin

Alves

Lopes

2010

Braz. arch. biol. technol.

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“…Humphreys et al (1990) obtuvieron una µ de 0.21 y 0.19 h -1 para B. bassiana, usando glucosa como fuente de carbono. Estas velocidades de crecimiento son 10 veces mayores que las obtenidas en el presente estudio usando melaza.…”

Section: Cinética De Crecimiento De Beauveria Bassianaunclassified

Estudio de las condiciones de mezclado en fermentador para la producción de blastosporas de Beauveria bassiana

García-Gutiérrez¹,

González-Maldonado²,

Medrano-Roldán³

et al. 2013

Rev. Colomb. Biotecnol.

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Estudio de las condiciones de mezclado en fermentador para la producción de ResumenSe caracterizaron tres fermentadores: New Brunswick M-19 de 14 litros, Applikon Biocontroller 1035 de 7 litros y New Brunswick Bioflo III de 7 litros, determinando el coeficiente volumétrico de transferencia de oxígeno (KLa), la retención de gas (RG) y el tiempo de mezclado (tM). El fermentador New Brunswick Bioflo III tuvo los mejores valores con una relación de diámetro del impulsor/diámetro del tanque (DI/DT) de 0.43, KLa = 9.5-208 h -1 y tM = 1.0-3.0 s, por lo que fue seleccionado para realizar la producción de blastosporas de Beauveria bassiana, utilizando melaza como fuente de carbono. Se estudiaron las condiciones de mezclado, utilizando diferentes combinaciones de impulsores tipo Rushton, Maxflo y Lightnin, bajo un diseño experimental factorial 3 2 . El tiempo de propagación fue de 4 días, el volumen de trabajo 4 litros, 10% de inóculo (1x10 6 blastosporas/ml), temperatura 30°C, agitación de 400-500 rpm, aireación de 0.5-1.0 vvm, y pH de 5.4.El hongo se desarrolló mejor utilizando la combinación de impulsores Rushton-Maxflo a 400 rpm y 1.0 vvm (F = 10.324, p ≤ 0.0123) (DMS=0.585), obteniendo una concentración de 1.2x10 9 blastosporas/ml, 2.2 g/l de biomasa y 2.48 g/l de consumo de sustrato (Y x/s=0.89). Las condiciones de mezclado y los parámetros obtenidos pueden ser aplicados en otros fermentadores para optimizar la producción de blastosporas de B. bassiana en la elaboración experimental de bioinsecticidas.Palabras clave: fermentación líquida, aireación, agitación, hongo entomopatógeno. AbstractIn this work three fermenters were characterized: New Brunswick M-19 of 14 liters, Biocontroller Applikon 1035 of 7 liters and New Brunswick Bioflo III of 7 liters, determining the volumetric coefficient oxygen transfer (KLa), gas hold up (GH) and the mixing time (tM). The fermenter New Brunswick Bioflo III had the best values with a ratio of diameter of the impeller/ vessel (DI/DT) of 0.43, KLa = 9.5-208 h -1 and tM = 1.0-3.0 s, so it was selected for the production of blastospores of Beauveria bassiana, using molasses as carbon source. We studied the mixing conditions, using different combinations of impellers, type Rushton, Lightnin and Maxflo under a factorial experimental design 3 2 . The propagation time was of 4 days, working volume 4l, 10% inoculum (1x10 6 blastospores/ml), temperature 30°C, agitation of 400-500 rpm, aeration 0.5-1.0 vvm, and pH of 5.4. The fungus growth better using a combination of impellers Rushton-Maxflo at 400 rpm and 1.0 vvm (F = 10.324, p ≤ 0.0123) (LSD = 0.585) obtaining a concentration of 1.2x10 9 blastospores/ml, 2.2 g/l biomass and 2.48 g/l of substrate consumption (Y x/s = 0.89). The mixing conditions and the parameters obtained can be applied to optimize the blastospore productions of B. bassiana to fermenter level in the experimental production of bioinsecticides.

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“…Until today, no products with BS or SCS of B. bassiana are available. A few reports on growth requirements and shake flask culture of B. bassiana strains show the best growth and germination in complex media (Bidochka et al [ 1987 ]; Chong-Rodríguez et al [ 2011 ]; Hegedus et al [ 1990 ]; Humphreys et al [ 1990 ]; Pham et al [ 2009 ]; Rombach [ 1989 ]; Safavi et al [ 2007 ]; Samsináková [ 1966 ]; Thomas et al [ 1987 ]; Vega et al [ 2003 ]). However, only a few publications deal with the production of mycelium (Núñez-Ramírez et al [ 2012 ]) and the production of BS in complex (Humphreys et al [ 1989 , 1990 ]) and mineral media (Lane et al [ 1991 ]) by submerged fermentation, respectively.…”

Section: Introductionmentioning

confidence: 99%

Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor

2014

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A novel approach for biological control of insect pests could be the use of the endophytic entomopathogenic Beauveria bassiana isolate ATP-02. For the utilization of the endophyte as a commercial biocontrol agent, the fungus has to be mass-produced. B. bassiana was raised in shake flask cultures to produce high concentrations of total spores (TS), which include blastospores (BS) and submerged conidiospores (SCS). The highest concentration of 1.33×109 TS/mL and the highest yield of 5.32×1010 TS/g sucrose was obtained in the TKI broth with 5% sugar beet molasses which consists of 50% sucrose as a carbon source. In spite of the lower sugar concentration (2.5%) the amount of TS could be increased up to 11-times in contrast to the cultivation with 5% sucrose. The scale-up to a 2 L stirred tank reactor was carried out at 25°C, 200–600 rpm and 1 vvm at pH 5.5. A TS yield of 5.2×1010 TS/g sucrose corresponding to a SCS yield of 0.2×1010 SCS/g sucrose was obtained after 216 h. With regards to the culture medium the cost of 1012 TS amounts to 0.24 €. Plutella xylostella larvae, which were fed with oilseed rape leaves treated with spores from fermentation resulted in 77 ± 5% mortality. Moreover, spores from submerged cultivation were able to colonize oilseed rape leaves via leaf application. This is the first report of fermentation of an endophytic B. bassiana strain in a low-cost culture medium to very high yields of TS.

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Comparison of sporulation of Paecilomyces farinosus and Beauveria bassiana in batch and fed-batch culture

Cited by 9 publications

References 11 publications

Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

Estudio de las condiciones de mezclado en fermentador para la producción de blastosporas de Beauveria bassiana

Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor

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