Comparison of supercritical fluid and ultrasound-assisted extraction of carotenoids and chlorophyll a from Dunaliella salina

Sánchez, María D. Macías; Mantell, Casimiro; Rodríguez, Miguel; Ossa, E.J. Martı́nez de la; Lubián, Luís M.; Montero, Olimpio

doi:10.1016/j.talanta.2008.07.032

Cited by 226 publications

(110 citation statements)

References 25 publications

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“…The highest chlorophyll a content, 18.09 (mg/g), was obtained for the extraction at 40 kHz and 40 • C for 4 h, and this value was much higher than 13.81 (mg/g) from a most conventional extraction process such as 70% ethanol at 80 • C for 24 h (data not shown). This concentration was also found to be higher than those reported from conventional extractions from Spirulina and even other plants [26,27]. This result strongly implies that the optimized ultrasonification extraction is an effective process for extracting chlorophyll a from natural resources, and the optimized conditions of this method can be expected to yield high amounts of intact chlorophyll.…”

Section: Resultsmentioning

confidence: 65%

Enhancement of Chlorophyll a Production from Marine Spirulina maxima by an Optimized Ultrasonic Extraction Process

Choi

Lee

2017

Applied Sciences

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Abstract:Under the optimal ultrasonification extraction conditions of 20.52 kHz for the frequency, 32.59 • C for the temperature, and 4.91 h for the process time, 17.98 mg/g of chlorophyll a was obtained. It was much higher than 13.81 mg/g from conventional 70% ethanol extraction and even higher than other data from Spirulina. This yield was close to the predicted value of 18.21 mg/g from the second-order polynomial model with a regression coefficient of 0.969. This model showed the greatest significance with the ultrasonic frequency and process time and the least significance with the temperature. The extracts also showed high α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activities as 69.38%, compared to 58.25% for the extracts from the 70% ethanol extraction. It was first shown that the optimal extraction was effective at enhancing the neuroprotective activities possibly due to the synergistic effects of higher amounts of chlorophyll a and other bioactive substances in the extract, revealing a 90% protection of the growth of mouse neuronal cells and a great reduction in Reactive Oxygen Species (ROS) production.

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Section: Resultsmentioning

confidence: 65%

Enhancement of Chlorophyll a Production from Marine Spirulina maxima by an Optimized Ultrasonic Extraction Process

Choi

Lee

2017

Applied Sciences

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“…The extraction process involves the organic solvent penetrating through the cell membrane and dissolving the lipids as well as the lipoproteins of chloroplast membranes [4]. It has been found that cell disruption, achieved through grinding, homogenisation, ultrasound or sonication, significantly improves the effectiveness of chlorophyll extraction using organic solvents [4,33,34,36]. Simon and Helliwell [36] found that, without cell disruption, only a quarter of the potential chlorophyll a was able to be extracted by an optimal method.…”

Section: Organic Solvent Extractionmentioning

confidence: 99%

“…Although 100% acetone was found not to yield the highest amount of chlorophyll from any particular species, its use as an extracting solvent strongly inhibited the formation of degradation products [4]. In studies carried out by Jeffrey et al [4] and Macías-Sánchez et al [33], dimethyl formamide (DMF) was found to be a superior extraction solvent to methanol, 90% ethanol, 100% ethanol and 90% acetone.…”

Section: Organic Solvent Extractionmentioning

confidence: 99%

Chlorophyll Extraction from Microalgae: A Review on the Process Engineering Aspects

Hosikian

Lim

Halim

et al. 2010

International Journal of Chemical Engineering

278

169

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Chlorophyll is an essential compound in many everyday products. It is used not only as an additive in pharmaceutical and cosmetic products but also as a natural food colouring agent. Additionally, it has antioxidant and antimutagenic properties. This review discusses the process engineering of chlorophyll extraction from microalgae. Different chlorophyll extraction methods and chlorophyll purification techniques are evaluated. Our preliminary analysis suggests supercritical fluid extraction to be superior to organic solvent extraction. When compared to spectroscopic technique, high performance liquid chromatography was shown to be more accurate and sensitive for chlorophyll analysis. Finally, through CO 2 capture and wastewater treatment, microalgae cultivation process was shown to have strong potential for mitigation of environmental impacts.

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“…Supercritical CO 2 (sc-CO 2 ) extraction has been widely applied in the food and pharmacy industry, as the process has the characteristics of inability to leave residual chemicals, environmental compatibility, and moderate operating temperatures and pressures [18][19][20]. Nevertheless, only few investigations are available on the extraction of chlorophyll a using sc-CO 2 extraction, as it is inefficient for nonpolar CO 2 to extract highly polar chlorophyll a.…”

Section: Introductionmentioning

confidence: 99%

Supercritical CO₂ Extraction of Chlorophyll a from Spirulina platensis with a Static Modifier

et al. 2010

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Supercritical CO 2 extraction with a static modifier was applied to extract chlorophyll a from Spirulina platensis. The effects of the process were investigated by single-factor and response surface analysis experiments. The optimal process parameters for supercritical CO 2 extraction were determined to be: ethanol/water as the modifier, 40 vol.-% water content in the modifier, 21.2 mL modifier volume, 1 h static soaking time, 2 h dynamic extraction time, 48.7 MPa extraction pressure, 326.4 K extraction temperature, and 10 g min -1 CO 2 flow rate. The optimized chlorophyll a extraction yield was 6.84 mg g -1. A comparison of the experimental results suggested that the yield of chlorophyll a by supercritical CO 2 extraction with modifier was higher than that obtained by conventional solvent extraction.

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Comparison of supercritical fluid and ultrasound-assisted extraction of carotenoids and chlorophyll a from Dunaliella salina

Cited by 226 publications

References 25 publications

Enhancement of Chlorophyll a Production from Marine Spirulina maxima by an Optimized Ultrasonic Extraction Process

Enhancement of Chlorophyll a Production from Marine Spirulina maxima by an Optimized Ultrasonic Extraction Process

Chlorophyll Extraction from Microalgae: A Review on the Process Engineering Aspects

Supercritical CO₂ Extraction of Chlorophyll a from Spirulina platensis with a Static Modifier

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Comparison of supercritical fluid and ultrasound-assisted extraction of carotenoids and chlorophyll a from Dunaliella salina

Cited by 226 publications

References 25 publications

Enhancement of Chlorophyll a Production from Marine Spirulina maxima by an Optimized Ultrasonic Extraction Process

Enhancement of Chlorophyll a Production from Marine Spirulina maxima by an Optimized Ultrasonic Extraction Process

Chlorophyll Extraction from Microalgae: A Review on the Process Engineering Aspects

Supercritical CO2 Extraction of Chlorophyll a from Spirulina platensis with a Static Modifier

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Product

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Supercritical CO₂ Extraction of Chlorophyll a from Spirulina platensis with a Static Modifier