Comparison of the adaptive potential of the Arthrobacter oxydans and Acinetobacter lwoffii isolates from permafrost sedimentary rock and the analogous collection strains

Kryazhevskikh, N. A.; Demkina, E. V.; Лойко, Н. Г.; Baslerov, R. V.; Колганова, Т. В.; Soina, V. S.; Manucharova, N. A.; Гальченко, В. Ф.; Эль-Регистан, Г. И.

doi:10.1134/s0026261713010050

Cited by 18 publications

(8 citation statements)

References 26 publications

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“…lwofii strains EK30A (a permafrost isolate) and BSW 27 (a collec tion strain) grown on TSB/2 medium were compared using the HCCA matrix. Analysis of the proteins of the cells of both species based on their m/z and intensity ( Table 2) revealed 23 common peaks, i.e., 54 and 26% of the total peak number, respectively (Table 2), which confirms the validity of the species identification of the permafrost isolate EK30A [19]. The intensity of the common peaks in the protein spectra of the cells of the collection strain BSW 27 exceeded that of the cells of permafrost isolate EK30A (Figs.…”

Section: Comparison the Maldi Spectra Of Bacteria Belongingsupporting

confidence: 60%

“…The dormant forms of Arthro bacter strains were obtained by growing them (i) in standard LB medium, with subsequent storage of the developed culture at 28°C for 2 months; (ii) in a nitro gen limited medium, with subsequent storage at 4°C for 2 months; and (iii) in a medium with a phosphate imbalance, with subsequent storage at 28°C for 2 months [19]. The DFs obtained in (i), (ii), and (iii) were referred to as DF S , DF N , and DF P , respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The variants obtained, which belonged to the dominant S type and to the minor R, M, and S m types, exhibited different physiological and biochemical features and adaptive properties [19].…”

Section: Monitoring the Maldi Spectra Of Bacterial Variantsmentioning

confidence: 97%

“…It was established earlier that the variant forming activ ity and the composition of the intrapopulation spec trum vary depending on the properties of DF formed under various conditions and not only on the medium composition used for DF inoculation [19,21,25,27,28]. Therefore, in order to analyze the MALDI spectra of the variants, they were obtained by plating bacterial DF formed under various conditions on a number of media with different composition.…”

Section: Monitoring the Maldi Spectra Of Bacterial Variantsmentioning

confidence: 99%

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Applicability of MALDI mass spectrometry for diagnostics of phase variants in bacterial populations

et al. 2015

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Efficiency of MALDI mass spectrometry for differentiation between phenotypic phase variants (in colony morphology and virulence/avirulence) was investigated for saprotrophic and opportunistically pathogenic bacteria of five genera (Acinetobacter, Arthrobacter, Rhodococcus, Corynebacterium, and Escheri chia). Analysis of MALDI spectra (on the SA and HCCA matrices) included (1) determination of similarity of the protein spectra as a percentage of the common protein peaks to the total amount of proteins, which reflects the phylogenetic relationships of the objects and has been recommended for identification of closely related species; (2) comparison of intensities of the common peaks; and (3) the presence of specific peaks as determinative characteristics of the variants. Under the standard analytical conditions, the similarity between the MALDI profiles was shown to increase in the row: genus-species-strain-variant. Assessment of inten sities of the common peaks was most applicable for differentiation between phase variants, especially in the case of high similarity of their profiles. Phase variants (A. oxydans strain K14) with similar colony morpho types (S, R, M, and S m ) grown on different media (LB agar, TSA, and TGYg) exhibited differences in their protein profiles reflecting the differences in their physiological characteristics. This finding is in agreement with our previous results on screening of the R. opacus with similar colony morphology and different substrate specificity in decomposition of chlorinated phenols. Analysis of MALDI spectra is probably the only efficient method for detection of such variants.

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Section: Comparison the Maldi Spectra Of Bacteria Belongingsupporting

confidence: 60%

Section: Methodsmentioning

confidence: 99%

“…The variants obtained, which belonged to the dominant S type and to the minor R, M, and S m types, exhibited different physiological and biochemical features and adaptive properties [19].…”

Section: Monitoring the Maldi Spectra Of Bacterial Variantsmentioning

confidence: 97%

Section: Monitoring the Maldi Spectra Of Bacterial Variantsmentioning

confidence: 99%

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Applicability of MALDI mass spectrometry for diagnostics of phase variants in bacterial populations

et al. 2015

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“…the 100% similarity occurred, while the strains had clear differences in colony morphology (data not shown) and physiology (Tables S3 and S4). This indicates that these strains are the intrapopulation variation, considering the data showed, that strains from natural habitats have high dissociation activity under stress conditions [63].…”

Section: Discussionmentioning

confidence: 72%

Soil bacterial communities of Sahara and Gibson deserts: Physiological and taxonomical characteristics

Belov¹,

Cheptsov²,

Vorobyova

2018

AIMS Microbiology

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The purpose of this research was to investigate the structure of soil bacteria communities present in the Gibson (Australia) and the Sahara (Egypt) deserts, as well as to estimate strain survivability under different environmental factors. It should be noticed that the screening of bacterial resistance to wide spectra of principally different stress conditions was performed for the first time. Experiments were conducted with culturable bacterial communities. Strains were identified using 16S rRNA sequencing, and stress-tolerance was estimated by growing strains in various nutrient media. In order to characterize the community the epifluorescent microscopy and multisubstrate testing were also performed. High bacterial abundance in the desert soils was detected, and there was seen a significant proportion of culturable cells. The close numbers of psychotropic and mesophilic bacteria in arid ecosystems were revealed. The representatives of the Actinobacteria phylum were dominant in the microbial communities, and Firmicutes , Proteobacteria , and Bacteroidetes phyla representatives were also identified. Tolerance of the axenic bacterial cultures, isolated from arid desert ecotopes, to temperature, pH, salts (KCl, NaCl, MgSO 4 , NaHCO 3 ), strong oxidizers (Mg(ClO 4 ) 2 ), and antibiotics (ampicillin, cephalexin, chloramphenicol, tetracycline, doxycycline, kanamycin, rifampicin) was studied. The bacterial isolates were characterized by polyextremotolerance and by the ability to maintain metabolic activity in vitro while influenced by a wide range of physicochemical and biotic factors.

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100 kGy gamma-affected microbial communities within the ancient Arctic permafrost under simulated Martian conditions

et al. 2017

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This research aimed to investigate the viability and biodiversity of microbial communities within ancient Arctic permafrost after exposure to a gamma-radiation dose of 100 kGy at low temperature (- 50 °C), low pressure (1 Torr) and dehydration conditions. The main objective was to assess the possibility for long-term survival of Earth-bound microorganisms in the subsurface of Martian regolith or inside small space bodies at constant absorption and accumulation of the gamma radiation dose. Investigated microbial communities had shown high resistance to a simulated Martian environment. After irradiation the total count of prokaryotic cells and number of metabolically active bacterial cells remained at the control level, while the number of bacterial CFUs decreased by 2 orders of magnitude, and the number of metabolically active cells of archaea decreased threefold. Besides, the abundance of culturable bacteria after irradiation was kept at a high level: not less than 3.7 × 10 cells/g. Potential metabolic activity of irradiated microbial communities in general were higher than in the control sample. A fairly high biodiversity of bacteria was detected in the exposed sample of permafrost, although the microbial community structure underwent significant changes after irradiation. In particular, actinobacteria populations of the genus Arthrobacter, which was not revealed in the control samples, became predominant in bacterial communities following the exposure. The results of the study testify that long-term preservation of microbial life inside Martian permafrost is possible. The data obtained can also be evaluated from the perspective of the potential for discovering viable Earth-bound microorganisms on other objects in the Solar system and inside of small bodies in outer space.

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Comparison of the adaptive potential of the Arthrobacter oxydans and Acinetobacter lwoffii isolates from permafrost sedimentary rock and the analogous collection strains

Cited by 18 publications

References 26 publications

Applicability of MALDI mass spectrometry for diagnostics of phase variants in bacterial populations

Applicability of MALDI mass spectrometry for diagnostics of phase variants in bacterial populations

Soil bacterial communities of Sahara and Gibson deserts: Physiological and taxonomical characteristics

100 kGy gamma-affected microbial communities within the ancient Arctic permafrost under simulated Martian conditions

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