The prevention of oxidative damage is important because it can lead to various disease conditions, such as hyperlipidemia, hypertension, and arrhythmia. Research on the protective effects of natural substances on H2O2treated H9c2 cells has been widely reported. However, little information is available on the preventive effect of Allomyrina dichotoma larva extract (ADLE) on oxidative damage. In this study, we investigated whether ADLE pretreatment has an antioxidative effect on H2O2-treated H9c2 cells. An MTT assay showed ADLE was non-cytotoxic and when co-treated decreased H2O2-induced cell death. DPPH and ABTS assays showed ADLE dose-dependently increased antioxidant activity, and FRAP assays showed ADLE increased reducing power. Furthermore, western blot revealed ADLE increased the level of proteins associated with apoptosis and antioxidant activity, and CAT and SOD assays showed their enzyme levels were higher in ADLE and H₂O₂ co-treated H9c2 cells than in H₂O₂-treated cells. The study shows that ADLE pretreatment prevents oxidative damage to H9c2 myocardial cells and has potential use as a functional material.