Comparison of the biological effects of anemia inducing and polycythemia inducing Friend virus complex

Steinheider, G.; Seidel, H.; Kreja, Ludwika

doi:10.1007/bf01963269

Cited by 30 publications

(15 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RV and FV-A-infected mice react with an increase in the relative proportion of differentiating erythroid cells, which still require erythropoietin (Hasthorpe, 1978;Opitz et al, 1977;Ostertag & Pragnell, 1981) but not those infected by FV-P (Tambourin, 1979;Fagg et al, 1980). Erythroid cells infected in vitro by FV-A require erythropoietin for maturation (Evans et al, 1980;Hankins & Troxler, 1980;Steinheider et al, 1979), but not those exposed to FV-P (Hankins & Troxler, 1980). RV rescued on infection of RA-1 cells by MuLV-F has similar properties to those described previously for RV with MuLV-R (Tables !…”

Section: Discussionsupporting

confidence: 49%

“…Bone marrow or spleen cells of mice infected with Friend anaemia virus (FV-A) show erythropoietin-independent erythroid colony formation in vitro. These colonies still require erythropoietin for full haemoglobinization, that is, for terminal erythroid differentiation (Fagg et al, 1980;Hankins & Troxler, 1980;Peschle et al, 1980;Steinheider et al, 1979). Infection of mice by MPSV induced an increase in both CFU-E-and BFU-E-type colonies but only in the presence of (low doses of) erythropoietin.…”

Section: Effect Of Rv On Erythroid Cell Proliferationmentioning

confidence: 99%

See 1 more Smart Citation

Rauscher Spleen Focus-forming Virus: Biological Properties and Relationship to Helper Viruses

Mol

Ostertag

Bilello³

et al. 1982

Journal of General Virology

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 49%

Section: Effect Of Rv On Erythroid Cell Proliferationmentioning

confidence: 99%

Rauscher Spleen Focus-forming Virus: Biological Properties and Relationship to Helper Viruses

Mol

Ostertag

Bilello³

et al. 1982

Journal of General Virology

View full text Add to dashboard Cite

“…Erythropoietin is required for colony formation by rel- (5,6,11,12). In contrast to CFU-FV, these colony-forming cells have limited proliferative capacity, are not tumorigenic, and appear early in the disease.…”

Section: Discussionmentioning

confidence: 99%

“…These assays have shown that, early after infection, the original Friend isolate (FV-A) induces anemia and an increase in the number of erythroid progenitor cells in the spleen which, like normal erythroid progenitors, require the presence of erythropoietin to proliferate and differentiate to form small hemoglobinized colonies in vitro (11)(12)(13)(14). In contrast, the polycythemia-inducing isolate of Friend virus (FV-P), derived from stocks of FV-A (15), causes an early increase in erythroid progenitor cells which can differentiate terminally in the absence of erythropoietin (5,6).…”

mentioning

confidence: 99%

Quantitative colony method for tumorigenic cells transformed by two distinct strains of Friend leukemia virus

Mager¹,

Mak²,

Bernstein³

1981

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

An in vitro colony method capable of detecting spleen cells malignantly transformed by Friend leukemia virus is described. These colony-forming cells, which form large erythroid colonies (10 4 -10 5 cells) in methylcellulose, can be detected late after infection with either the anemia-inducing (FV-A) or polycythemia-inducing (FV-P) isolates of Friend virus. Colony formation by these cells is dependent only on fetal calf serum as an exogeneous growth factor. The presence of these colony-forming cells in FV-P-infected spleens could not be detected until at least 3 weeks after virus infection, even though the most rapid increase in spleen weight occurred earlier, between 1 and 2 weeks after infection. Thereafter, the numbers of colony-forming cells increased sharply up to 5 weeks after infection with FV-P, beyond which time the mice generally did not survive. After infection with FV-A, colony-forming cells were detected only at 8-12 weeks and their numbers generally increased thereafter. Permanent cell lines were established from a significant fraction of FV-P and FV-A-induced colonies, and these cell lines could be chemically induced to synthesize hemoglobin. All individual colonies produced complete Friend virus complex. However, virus production appeared to decline in at least some cell lines. Both FV-P- and FV-A-induced colonies contained cells capable of forming spleen colonies in irradiated recipients and subcutaneous tumors in unirradiated mice. Thus, the assay method described here appears to detect a unique class of malignant Friend virus-transformed cells that can be detected only in the advanced stages of Friend virus-induced erythroleukemia.

show abstract

“…Friend virus is a complex of two viruses: a defective spleen focus forming virus (SFFV), and a replication-competent Friend murine leukaemia virus (F-MuLV). The SFFV component is responsible for the acute leukaemia in adult mice, and exists in two forms: SFFVa which causes anaemia (Steinheider et al, 1979;Tambourin et al, 1979) and SFFVp which causes polycythaemia (Mirand et al, 1968;Tambourin & Wendling, 1971).…”

mentioning

confidence: 99%

Erythroblasts from Friend virus infected‐ and phenylhydrazine‐treated mice accurately model erythroid differentiation

Hodges¹,

Winter²,

Lappin³

1999

Br J Haematol

View full text Add to dashboard Cite

Summary. The dynamics of gene expression during terminal erythroid differentiation have been examined in three murine models; the erythroleukaemia cell line HCD-57 and splenic erythroblasts isolated from mice treated with either the anaemia-inducing strain of Friend virus (FVA cells) or the haemolytic agent phenylhydrazine (PHZ cells). In response to erythropoietin (EPO) and haemin, HCD-57 cells proliferated and synthesized haemoglobin, but failed to complete terminal differentiation as indicated by lack of change in both gene expression and morphological appearance. In contrast, EPO-induced terminal differentiation in FVA and PHZ cells in vitro was accompanied by increases in haemoglobin positivity, morphological maturation and a shared pattern of gene expression. EPO receptor (EPO-R) mRNA levels peaked before globin gene expression which was maximal at 24 h. Peak GATA-1 and EKLF mRNA levels also preceded the globin gene peak, but the highest NF-E2 levels coincided with maximal globin levels, suggesting a role for NF-E2 in the maintenance, rather than the initiation of globin gene expression. Peak expression of d-aminolaevulinic acid synthase (ALAS) coincided with peak globin expression. FVA and PHZ cells represent more effective models than the HCD-57 cell line for the investigation of erythroid gene expression during EPO-regulated terminal erythropoiesis.

show abstract

Comparison of the biological effects of anemia inducing and polycythemia inducing Friend virus complex

Cited by 30 publications

References 11 publications

Rauscher Spleen Focus-forming Virus: Biological Properties and Relationship to Helper Viruses

Rauscher Spleen Focus-forming Virus: Biological Properties and Relationship to Helper Viruses

Quantitative colony method for tumorigenic cells transformed by two distinct strains of Friend leukemia virus

Erythroblasts from Friend virus infected‐ and phenylhydrazine‐treated mice accurately model erythroid differentiation

Contact Info

Product

Resources

About