Comparison of the Biological Properties of Plasma-Treated Solution and Solution of Chemical Reagents

Abstract

Presently, plasma-treated solutions (PTS) are widely introduced into medicine. Plasma-activated solutions contain various reactive forms of oxygen and nitrogen which provide the desired biological effects. Yet it remains unclear exactly which components of the treated solution are the most important and what the difference is between the plasma-treated solution and a chemically prepared solution composed of the same components. In this work, we show that the chemically prepared mixture of nitrites, nitrates, and hydrogen peroxide with concentrations similar to the plasma-treated solution exerts a fundamentally different effect on a cell culture. The chemically prepared solution has higher cyto- and genotoxicity and causes necrosis, while under the action of the plasma-treated solution, apoptotic processes develop in the cells slowly.

Enhancement of the Plant Grafting Technique with Dielectric Barrier Discharge Cold Atmospheric Plasma and Plasma-Treated Solution

“…For the quantitative determination of hydrogen peroxide in aqueous solutions, a highly sensitive method of enhanced chemiluminescence in the luminol-p-iodophenol-horseradish peroxidase system was used [ 69 , 70 , 71 ]. The luminescence intensity was determined using a Biotox-7A chemiluminometer (ANO ICE, Moscow, Russia).…”

The Role of Autophagy and Apoptosis in the Combined Action of Plasma-Treated Saline, Doxorubicin, and Medroxyprogesterone Acetate on K562 Myeloid Leukaemia Cells

“…However, in this work, serious differences in the concentration of nitrites and hydrogen peroxide, as well as in pH and redox potential, did not lead to such differences in the biological effect for NaCl solution, Ringer’s solution, and HBSS. It was previously shown that a solution treated with cold plasma to form hydrogen peroxide, nitrites, and nitrates has a lower cytotoxic effect than a chemically prepared solution of hydrogen peroxide, nitrites, and nitrates at the same concentrations [ 42 , 43 ]. It is possible that any particular biological effects are caused not by specific reactive oxygen and nitrogen species (RONS) but by their specific concentration ratios and molecular energies.…”

Bacterial Decontamination of Water-Containing Objects Using Piezoelectric Direct Discharge Plasma and Plasma Jet

“…With increasing time of solution treatment with PDD, an increase in autophagy activation is observed in three types of cells: mononuclear leukocytes from apparently healthy donors, myeloid leukemia cell line K562, and T-lymphoblastic leukemia cell line Jurkat. It was also shown that a chemically prepared mixture of nitrites, nitrates, and hydrogen peroxide in concentrations similar to PTS has an effect on cell culture that is fundamentally different from the effect of PTS [38]. A chemically prepared solution is more cyto-and genotoxic and causes necrosis, while under the influence of PTS, apoptotic processes slowly start in the cells.…”