A garden plant grafting technique enhanced by cold plasma (CAP) and plasma-treated solutions (PTS) is described for the first time. It has been shown that CAP created by a dielectric barrier discharge (DBD) and PTS makes it possible to increase the growth of Pyrus communis L. by 35–44%, and the diameter of the root collar by 10–28%. In this case, the electrical resistivity of the graft decreased by 20–48%, which indicated the formation of a more developed vascular system at the rootstock–scion interface. The characteristics of DBD CAP and PTS are described in detail.

Presently, plasma-treated solutions (PTS) are widely introduced into medicine. Plasma-activated solutions contain various reactive forms of oxygen and nitrogen which provide the desired biological effects. Yet it remains unclear exactly which components of the treated solution are the most important and what the difference is between the plasma-treated solution and a chemically prepared solution composed of the same components. In this work, we show that the chemically prepared mixture of nitrites, nitrates, and hydrogen peroxide with concentrations similar to the plasma-treated solution exerts a fundamentally different effect on a cell culture. The chemically prepared solution has higher cyto- and genotoxicity and causes necrosis, while under the action of the plasma-treated solution, apoptotic processes develop in the cells slowly.

The anti-cancer properties of plasma-treated solutions (PTS) and their interaction with drugs are one of the most popular topics in modern plasma medicine. Our research involved comparing the effects of four physiological saline solutions (0.9% NaCl, Ringer’s solution, Hank’s Balanced Salt Solution, Hank’s Balanced Salt Solution with amino acids added in concentrations observed in the human blood) treated with cold atmospheric plasma and studying the combined cytotoxic effect of PTS with doxorubicin and medroxyprogesterone acetate (MPA). Analysis of the effect of the studied agents on the formation of radicals in the incubation medium, the vitality of K562 myeloid leukaemia cells, and the processes of autophagy and apoptosis in them revealed two key findings. The first is that when using PTS and doxorubicin-containing PTS, autophagy is the predominant process in cancer cells. The second is that combining PTS with MPA enhances apoptotic processes. It was hypothesised that while autophagy is stimulated by the accumulation of reactive oxygen species in the cell, apoptosis is stimulated through specific cell progesterone receptors.

Cold plasma and plasma-treated solutions (PTS) have recently become widely adopted for the treatment of cancer and inflammatory diseases, particularly in concert with other medications. These complexes affect cytokine and oxidative activity of leukocytes, which are important in the pathogenesis of a variety of diseases. In this work, we study the variations in the immune response of healthy leukocytes when doxorubicin, medroxyprogesterone acetate, and PTS affect them separately or in combination. Employing the "CAPKO-JET" plasma source, we achieve an increase in the anti-tumor immune response from the combination of PTS with doxorubicin and MPA at treatment times shorter than 2.5 min. In contrast, the anti-inflammatory immune response is amplified at treatment times of 7.5 min or more. Applying PTS without the above medications upon the completion of the first phase of the inflammation therapy at the injury site is suggested to accelerate healing.