Comparison of the characterization on binding of alpinetin and cardamonin to lysozyme by spectroscopic methods

He, Wei; Li, Ying; Tang, Jianghong; Luan, Feng; Jin, Jing; Hu, Zhide

doi:10.1016/j.ijbiomac.2005.11.003

Cited by 55 publications

(26 citation statements)

References 16 publications

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“…However, other studies concerning the interaction of flavonoids detected small secondary structure changes, as well as tertiary structure changes, by the flavonoids alpinetin and cardamonin [7]. Our findings reveal that alterations in protein secondary structure were not found with either luteolin or apigenin, by FTIR or fluorescence spectrometry, suggesting that the alterations in rate of hydrogen deuterium exchange is mainly due to changes in the tertiary structure.…”

Section: Discussioncontrasting

confidence: 44%

“…As it can be seen in the absorbance spectra and second derivatives, the amide I band showed the same peak and shape in the presence and absence of the ligands for both proteins. As the amide I is composed of the sum of β-turn, β-sheet and α-helix absorbance contributions [7,10], it can be concluded that the presence of P. barbatus extract or its metabolites did not interfere with the secondary structure of HSA or lysozyme.…”

Section: Determination Of Protein Structure Changes Caused By P Barbmentioning

confidence: 99%

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Interaction betweenPlectranthus barbatusherbal tea components and human serum albumin and lysozyme: Binding and activity studies

et al. 2011

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Abstract. Anti-cholinesterase and antioxidant active constituents of Plectranthus barbatus aqueous extract were found in plasma of rats after its administration -rosmarinic acid, luteolin and apigenin. The aim of the present work is to determine if the extract components can interact with human plasma proteins, namely albumin and lysozyme. Protein intrinsic fluorescence analysis showed that the plant phenolic compounds may bind to albumin, the main transport protein in plasma, and to lysozyme. The estimated thermodynamic parameters suggest that the main intermolecular interaction is hydrophobic association. FTIR analysis of the protein amide bands showed that the plant extract components do not alter the secondary structure of either albumin or lysozyme, however the rate of hydrogen-deuterium exchange suggests that tertiary structure changes might have occurred. An increase of hydrogen deuterium exchange suggests that rosmarinic acid may bind to the fatty acid binding sites in albumin, while luteolin and apigenin may bind to the drug binding sites. The plant extract components also inhibit lysozyme activity with IC 50 values around 100 µM. Therefore P. barbatus herbal tea, rosmarinic acid, luteolin and apigenin interact and may be transported by albumin and lysozyme. The inhibition of lysozyme activity may be an additional mechanism for its anti-inflammatory activity.

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Section: Discussioncontrasting

confidence: 44%

Section: Determination Of Protein Structure Changes Caused By P Barbmentioning

confidence: 99%

Interaction betweenPlectranthus barbatusherbal tea components and human serum albumin and lysozyme: Binding and activity studies

et al. 2011

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“…Studies concerning the binding of cardamonin to serum albumin (the most abundant protein in human plasma, very important in the systemic transport of many relevant substances) had already been performed, 93 results lead to the conclusion that it binds site II (with an enthalpy change DH 0 of -25.3 kJ/mol and an entropy change DS 0 of 7.04 J mol -1 K -1 ). The binding to lysozyme has also been studied by spectroscopic methods 94 : enthalpy change DH 0 of -12.1 kJ/ mol and an entropy change DS 0 of 51.5 J mol -1 K -1 . These are relevant information to the development of chalconebased medication.…”

Section: Miscellaneousmentioning

confidence: 99%

An Overview on Cardamonin

Gonçalves

Valente

Rodrigues

2014

Journal of Medicinal Food

109

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Cardamonin, as shown by the increasing number of publications, has received growing attention from the scientific community due to the expectations toward its benefits to human health. In this study, research on cardamonin is reviewed, including its natural sources, health promoting aspects, and analytical methods for its determination. Therefore, this article hopes to aid current and future researchers on the search for reliable answers concerning cardamonin's value in medicine.

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“…In addition, previous studies have identified these compounts to exhibit significant antitumour effects (6)(7)(8). Alpinetin is a natural flavonoid, primarily found in Zingiberaceae, including turmeric, cardamom and radix curcumae (9). A number of studies have demonstrated that alpinetin has marked antitumour and inhibitory effects on tumour cell proliferation and inhibits the growth of numerous types of tumour cells, including breast, colon, lung, cervical and liver cancer cells (10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase

Wang

Lü

et al. 2012

Molecular Medicine Reports

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Abstract. Alpinetin is a natural flavonoid widely distributed inZingiberaceae. Previous studies have demonstrated that alpinetin markedly inhibits tumour growth. However, the molecular mechanisms underlying the antitumour effects of alpinetin are unclear. Bcl-2-associated X protein (Bax) translocation is known to activate the mitochondrial-dependent endogenous apoptosis pathway. The aim of the current study was to investigate the roles of Bax and the mitochondrial pathway during alpinetin-induced gastric cancer cell apoptosis and the effects of alpinetin on the cell cycle. Human gastric cancer cells were treated with various doses of alpinetin and an MTT assay was performed to measure cell viability, flow cytometry to measure the apoptotic rate, changes in the cell cycle and mitochondrial membrane potential and western blot analysis to detect the expression levels of relevant proteins. Results demonstrate that alpinetin induces apoptosis in human gastric cancer cells in a dose-and time-dependent manner. During the early stages of apoptosis, alpinetin may alter mitochondrial membrane potential leading to release of cytochrome c from mitochondria, activation of caspase family members and ultimately apoptosis of human gastric cancer cells. Results of the present study indicate that alpinetin-induced human gastric cancer cell apoptosis is associated with the mitochondrial pathway.

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Comparison of the characterization on binding of alpinetin and cardamonin to lysozyme by spectroscopic methods

Cited by 55 publications

References 16 publications

Interaction betweenPlectranthus barbatusherbal tea components and human serum albumin and lysozyme: Binding and activity studies

Interaction betweenPlectranthus barbatusherbal tea components and human serum albumin and lysozyme: Binding and activity studies

An Overview on Cardamonin

Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase

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