Comparison of the cis-acting control regions of two coordinately controlled genes involved in ethanol utilization in Aspergillus nidulans

Gwynne, David I.; Buxton, Frank P.; Sibley, Susan; Davies, R. Wayne; Lockington, Robin; Scazzocchio, Claudio; Sealy-Lewis, Heather M.

doi:10.1016/0378-1119(87)90309-x

Cited by 111 publications

(60 citation statements)

References 46 publications

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“…Overexpression of sfgA inhibits conidiation: To further verify the repressive role of sfgA in conidiation and ST production we generated the sfgA overexpression mutant by fusing the sfgA ORF with the inducible alcA promoter (Gwynne et al 1987). Multiple strains with a single-copy integration of the alcA(p)TsfgA fusion construct were generated and expression of sfgA was controlled by varying carbon sources: e.g., glucose (noninducing) or threonine (inducing).…”

Section: H48qv49fmentioning

confidence: 99%

“…The sfgA overexpression mutant was generated by a PCR-assisted method as described (Yuet al 2004). Briefly, the alcA promoter (Gwynne et al 1987) and the sfgA ORF with terminator were amplified using the primer pairs OJA106-107 and OYG11-28, respectively, and fused as described . The alcA(p)TsfgA fusion product was amplified with OJA108 and OYG29, and the final amplicon was digested with BamHI and ligated into the BamHI cut pSH96 plasmid (Wieser and Adams 1995).…”

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confidence: 99%

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FluG-Dependent Asexual Development in Aspergillus nidulans Occurs via Derepression

Seo¹,

2006

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The asexual spore is one of the most crucial factors contributing to the fecundity and fitness of filamentous fungi. Although the developmental activator FluG was shown to be necessary for activation of asexual sporulation (conidiation) and production of the carcinogenic mycotoxin sterigmatocystin (ST) in the model filamentous fungus Aspergillus nidulans, the molecular mechanisms underlying the developmental switch have remained elusive. In this study, we report that the FluG-mediated conidiation in A. nidulans occurs via derepression. Suppressor analyses of fluG led to the identification of the sfgA gene encoding a novel protein with the Gal4-type Zn(II) 2 Cys 6 binuclear cluster DNA-binding motif at the N terminus. Deletion (D) and 31 other loss-of-function sfgA mutations bypassed the need for fluG in conidiation and production of ST. Moreover, both DsfgA and DsfgA DfluG mutations resulted in identical phenotypes in growth, conidiation, and ST production, indicating that the primary role of FluG is to remove repressive effects imposed by SfgA. In accordance with the proposed regulatory role of SfgA, overexpression of sfgA inhibited conidiation and delayed/reduced expression of conidiation-and STspecific genes. Genetic analyses demonstrated that SfgA functions downstream of FluG but upstream of transcriptional activators (FlbD, FlbC, FlbB, and BrlA) necessary for normal conidiation.

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Section: H48qv49fmentioning

confidence: 99%

mentioning

confidence: 99%

FluG-Dependent Asexual Development in Aspergillus nidulans Occurs via Derepression

Seo¹,

2006

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“…The alcA promoter activates gene expression in the presence of carbon sources such as ethanol, glycerol, and threonine and represses it in the presence of glucose. 25) Accordingly, after 24 h of culture in YPD liquid medium (1% glucose), mpkB transcript levels were lower in the CmpkB strains than in the wild-type strain (Fig. S4, left).…”

Section: Construction Of Conditional-mpkb Strains Withmentioning

confidence: 95%

Mitogen-activated protein kinases MpkA and MpkB independently affect micafungin sensitivity in Aspergillus nidulans

Yoshimi

Fujioka

Mizutani

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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The transcriptional regulation of the MAPK mpkA and cell wall-related genes in Aspergillus nidulans differs from that of their counterparts in Saccharomyces cerevisiae. The A. nidulans MAPK MpkB is putatively orthologous to the yeast MAPKs Kss1p and Fus3p. To investigate MpkB and its contribution to cell wall integrity in A. nidulans, we constructed mpkB-disruptant (mpkB∆) strains. We previously showed that mpkA∆ strains exhibited reduced colony growth and increased sensitivity to the β-1,3-glucan synthase inhibitor micafungin. Like mpkA∆ strains, mpkB∆ strains exhibited slight growth retardation and increased sensitivity to micafungin. Although MpkB-dependent signaling modulated the transcription of some cell wall-related genes, the sugar composition of cell wall fractions was similar among wild-type, mpkA∆, and mpkB∆ strains. To elucidate the relationship between MpkA and MpkB pathways, we compared conditional mutants of mpkB with those with mpkA deletion. Sensitivity testing suggested that MpkA and MpkB additively contribute to micafungin activity in A. nidulans.

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“…The probes used were the entire genes of aleR, alcA and aldA cloned into the Bluescript plasmid [20][21][22]. The membranes were hybridized with a restriction fragment corresponding to the actin gene as an internal control to monitor the amount of specific mRNAs relative to that of actin mRNA.…”

Section: Isolation Of Rna and Quantitative Analysismentioning

confidence: 99%

The basal level of transcription of the alc genes in the ethanol regulon in Aspergillus nidulans is controlled both by the specific transactivator AlcR and the general carbon catabolite repressor CreA

1995

FEBS Letters

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In the A. nidulans ethanol utilization pathway, specific induction is mediated by the transactivator AIcR which is subject to strong positive autogenous regulation and activates the transcription of the two structural genes alcA and aldA. Carbon catabolite repression is mediated by CreA which represses directly the transacting gene alcR and the two structural genes. We show here that the basal expression of the alcR and alcA genes is also controlled by the two regulatory circuits, positively by the transactivator AIcR and negatively by the repressor CreA, the aldA gene being subject only to the control of the CreA repressor.

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Comparison of the cis-acting control regions of two coordinately controlled genes involved in ethanol utilization in Aspergillus nidulans

Cited by 111 publications

References 46 publications

FluG-Dependent Asexual Development in Aspergillus nidulans Occurs via Derepression

FluG-Dependent Asexual Development in Aspergillus nidulans Occurs via Derepression

Mitogen-activated protein kinases MpkA and MpkB independently affect micafungin sensitivity in Aspergillus nidulans

The basal level of transcription of the alc genes in the ethanol regulon in Aspergillus nidulans is controlled both by the specific transactivator AlcR and the general carbon catabolite repressor CreA

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