Comparison of the Cisterna Maturation-Progression Model with the Kiss-and-Run Model of Intra-Golgi Transport: Role of Cisternal Pores and Cargo Domains

Beznoussenko, Galina V.; Kweon, Hee‐Seok; Сесорова, И. С.; Mirоnоv, Alexander A.

doi:10.3390/ijms23073590

Cited by 10 publications

(37 citation statements)

References 118 publications

(261 reference statements)

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“…2 of (Hudoklin et al 2009 )). This situation is similar to the situation in neurons, where the cis -most cisterna joins the Golgi stacks and is the opposite to the situation in fibroblasts, where the trans -most cisterna joins the Golgi stack (Mironov et al 2017 ; Beznoussenko et al 2022 ). However, it seems that the Golgi complex contains only limited amount of UPs.…”

Section: Summary and Open Questionsmentioning

confidence: 55%

“…This suggests that COPI vesicles are not involved in the transport of UP particles through the Golgi complex. On the other hand, it is known that COPI vesicles do not participate in the recycling of Golgi resident proteins (Mironov and Beznoussenko 2019 ; Beznoussenko et al 2022 ) and UP particles are rather big for diffusion along the membrane continuum within Golgi cisternae.…”

Section: Summary and Open Questionsmentioning

confidence: 99%

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The Golgi complex: An organelle that determines urothelial cell biology in health and disease

Kreft

Mirоnоv

Hudoklin

2022

Histochem Cell Biol

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The Golgi complex undergoes considerable structural remodeling during differentiation of urothelial cells in vivo and in vitro. It is known that in a healthy bladder the differentiation from the basal to the superficial cell layer leads to the formation of the tightest barrier in our body, i.e., the blood–urine barrier. In this process, urothelial cells start expressing tight junctional proteins, apical membrane lipids, surface glycans, and integral membrane proteins, the uroplakins (UPs). The latter are the most abundant membrane proteins in the apical plasma membrane of differentiated superficial urothelial cells (UCs) and, in addition to well-developed tight junctions, contribute to the permeability barrier by their structural organization and by hindering endocytosis from the apical plasma membrane. By studying the transport of UPs, we were able to demonstrate their differentiation-dependent effect on the Golgi architecture. Although fragmentation of the Golgi complex is known to be associated with mitosis and apoptosis, we found that the process of Golgi fragmentation is required for delivery of certain specific urothelial differentiation cargoes to the plasma membrane as well as for cell–cell communication. In this review, we will discuss the currently known contribution of the Golgi complex to the formation of the blood–urine barrier in normal UCs and how it may be involved in the loss of the blood–urine barrier in cancer. Some open questions related to the Golgi complex in the urothelium will be highlighted.

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Section: Summary and Open Questionsmentioning

confidence: 55%

Section: Summary and Open Questionsmentioning

confidence: 99%

The Golgi complex: An organelle that determines urothelial cell biology in health and disease

Kreft

Mirоnоv

Hudoklin

2022

Histochem Cell Biol

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“…Samples were taken from 106 rats, namely, 25 normal male WKY rats subjected to standard cryoinjury, 55 male WKY and SHR rats subjected to other more complicated experiments, and 26 normal male WKY rats subjected to correlative light electron microscopy and immuno-electron microscopy labeling. Methodology and the details of all ethics rules are described in [ 50 , 51 , 52 ]. Briefly, all the experimental animal procedures were approved by the Committees of the Ivanovo State Medical Academy.…”

Section: Methodsmentioning

confidence: 99%

Role of Endothelial Regeneration and Overloading of Enterocytes with Lipids in Capturing of Lipoproteins by Basement Membrane of Rat Aortic Endothelium

et al. 2022

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Atherosclerosis is a complex non-monogenic disease related to endothelial damage in elastic-type arteries and incorrect feeding. Here, using cryodamage of endothelial cells (ECs) of rat abdominal aorta, we examined the role of the EC basement membrane (BM) for re-endothelization endothelial regeneration and its ability to capture low density lipoproteins (LDLs). Regeneration of endothelium induced thickening of the ECBM. Secretion of the BM components occurred in the G2-phase. Multiple regenerations, as well as arterial hypertension and aging, also led to the thickening of the BM. Under these conditions, the speed of re-endothelialization increased. The thick BM captured more LDLs. LDLs formed after overloading of rats with lipids acquired higher affinity to the BM, presumably due to the prolonged transport of chylomicrons through neuraminidase-positive endo-lysosomes. These data provide new molecular and cellular mechanisms of atherogenesis.

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“…Brain cortex was excised and processed for EM examination and EM tomography as previously described (Beznoussenko et al, 2015). For immuno-labeling, the grids were prepared as previously described (Beznoussenko et al, 2022). Briefly, the grids were incubated for 10 min in 0.5% BSA-c in PBS (bovine serum albumin-c TM , acetylated, 10% in water; Aurion) to prevent nonspecific labeling, and then incubated on 10 μL droplets of primary anti-Gpr126 (ab218046; Abcam) diluted 1:10 for 2 h, and then with protein-A gold 10 nm (PAG10, CMC, Utrecht, The Netherlands) 1:50 diluted in blocking solution for 20 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Dual role of brain endothelial Gpr126 in blood-brain barrier development and ischemic stroke

Kakogiannos

Scalise

Martini

et al. 2022

Preprint

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The blood–brain barrier (BBB) acquires unique properties for regulation of the neuronal function during development. The genesis of the BBB coupled with angiogenesis is orchestrated by the Wnt/β–catenin signaling pathway. Aside from the importance of Wnt/β–catenin signaling, the molecular mechanisms that regulate these processes are poorly understood. Here, we identify the brain endothelial adhesion G–protein–coupled receptor Gpr126 as a novel target gene of Wnt/β–catenin signaling that is required for postnatal BBB development, and its expression is detrimental for ischemic stroke in adults. We show that Gpr126 expression is high in mouse brain endothelium during BBB formation, but decreases in the adult. Inactivation of Gpr126 in postnatal endothelial cells results in vessel enlargement and impairs acquisition of the BBB characteristics, such as increased neurovascular permeability, and reduced basement membrane protein deposition and pericyte coverage. Mechanistically, Gpr126 is required during developmental angiogenesis to promote endothelial cell migration, acting via an interaction between Lrp1 and β1–integrin, which couples vessel morphogenesis to BBB formation. Interestingly, in adult mice with an established BBB, the lack of Gpr126 expression in acute ischemic stroke is protective and coupled with reduced microglia activation, which contributes to an improved neurological outcome. These data identify Gpr126 as a promising therapeutic target to treat ischemic stroke.

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Comparison of the Cisterna Maturation-Progression Model with the Kiss-and-Run Model of Intra-Golgi Transport: Role of Cisternal Pores and Cargo Domains

Cited by 10 publications

References 118 publications

The Golgi complex: An organelle that determines urothelial cell biology in health and disease

The Golgi complex: An organelle that determines urothelial cell biology in health and disease

Role of Endothelial Regeneration and Overloading of Enterocytes with Lipids in Capturing of Lipoproteins by Basement Membrane of Rat Aortic Endothelium

Dual role of brain endothelial Gpr126 in blood-brain barrier development and ischemic stroke

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