Comparison of the CMNC and MNC apheresis protocol for the collection of T‐cells showed comparable outcome: An observational study in a single centre

Putensen, Daniel; Smith, Richard J.; Pilcher, Linda; Trandafir, George

doi:10.1002/jca.21618

Cited by 13 publications

(9 citation statements)

References 18 publications

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“…The mean PLT attrition rate associated with apheresis using the Spectra Optia CMNC protocol was 48% (33.5%‐66.5%). Consistent with our results, previous human and canine apheresis studies described a decrease in PLT count (27.0%‐44.7%) after apheresis compared with the count before apheresis . Loss of PLTs is an adverse effect commonly associated with apheresis.…”

Section: Discussionsupporting

confidence: 91%

Comparison of three mobilization protocols for peripheral blood stem cell apheresis with Spectra Optia continuous mononuclear cell protocol in healthy dogs

Kim

Hosoya

Kobayashi

et al. 2018

Vet Comparative Oncology

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Peripheral blood stem cell (PBSC) transplantation following consolidation therapy is a feasible treatment option for canine haematological malignancies. In veterinary medicine, haematopoietic stem cells are generally mobilized into peripheral circulation using a granulocyte colony-stimulating factor (G-CSF). This pilot study aimed to evaluate the haematopoietic stem cell mobilization effect of three different regimens for PBSC apheresis with Spectra Optia continuous mononuclear cell (CMNC) protocol in healthy dogs. Stem cell mobilization was performed using high-dose plerixafor (CXCR-4 inhibitor) alone, a G-CSF alone, or a combination of the low-dose plerixafor and G-CSF. Three dogs were assigned to each mobilization protocol. Regardless of the mobilization protocol, the total blood volume processed was uniformly set as 270 mL/kg and many PBSCs, defined as CD34+/CD45 cells, within the apheresis product were compared. Changes in complete blood count, PBSC counts, and blood chemistry analysis were monitored before, during, and after apheresis. All dogs tolerated the apheresis procedure using the Spectra Optia system with minimal adverse effects. The mean PBSC counts of the apheresis products for plerixafor, G-CSF, and the combination groups were 1.3 ± 0.24, 4.2 ± 0.47, and 6.4 ± 0.9 × 10 cells/kg, respectively. The apheresis procedure using Spectra Optia CMNC protocol in dogs is safe and feasible. Furthermore, PBSC mobilization with a combination of G-CSF and plerixafor appeared more effective than either compound alone in mobilizing PBSC to the peripheral blood in dogs.

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Section: Discussionsupporting

confidence: 91%

Comparison of three mobilization protocols for peripheral blood stem cell apheresis with Spectra Optia continuous mononuclear cell protocol in healthy dogs

Kim

Hosoya

Kobayashi

et al. 2018

Vet Comparative Oncology

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“…The authors reported that the MNC program was associated with higher lymphocyte concentration in the final product [17], as found in our series. Putensen et al also compared CMNC and MNC for T cell collection in adults [18]; however, they observed similar efficiency (CE2%) between CMNC (54.7%) compared with MNC (50.4%).…”

Section: Discussionmentioning

confidence: 99%

Extracorporeal photopheresis in paediatric patients: A retrospective comparison between different ‘off‐line’ protocols

et al. 2022

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Background and Objectives: Extracorporeal photopheresis (ECP) has been shown to be an effective treatment for graft-versus-host disease (GvHD).However, information regarding lymphocyte collection for ECP in children is limited. The aim of this study was to analyse and compare lymphocyte collection for ECP in children using different devices and protocols. Moreover, we have studied both safety and variables of the infused product related to treatment efficacy.Patients and Methods: This was a retrospective study of 91 patients who underwent 1524 apheresis procedures with either the COBE Spectra or Spectra Optia system. The comparison study between the Optia protocols (MNC and CMNC) was prioritized. We analysed 578 procedures using the Optia blood cell separator: 204 and 374 using the MNC and the CMNC protocol, respectively.Results: The Optia CMNC protocol showed better collection efficiency, with increased lymphocyte collection per kg of body weight (p < 0.001). On multivariate analysis, the type of protocol showed no relationship with haematocrit or platelet loss. Most procedures were well-tolerated, with the most frequent adverse events related to venous access (21.7%). Seventy-one percent of patients had either partial or complete clinical GvHD response. In the multivariate model, only two variables were associated with a better response to ECP, younger age and a greater increase of B lymphocytes after treatment. Conclusion:Lymphocyte collection for ECP is well-tolerated in most children, achieving complete or partial response in more than half of GvHD patients. CMNC is the optimal software to perform lymphocyte collection in children.

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“…With the unavailability of the Cobe Spectra, the Spectra Optia has been validated to a great extent in regard to primary, clinical properties of the apheresis products, and effects on clinical outcomes have been reported . Existing studies to address the potential impact of collection programs on later processing of these products focused on stem cells, used random donor cohorts, or analyzed the apheresis products without further functional testing . The impact of the apheresis method on downstream applications in research and clinical trials with cellular products that often include extensive handling (isolation, cryoconservation, enrichment, culture) has not yet been investigated.…”

Section: Discussionmentioning

confidence: 99%

Impact of collection programs for the generation of monocyte apheresis products on product quality and composition as starting material for the generation of cellular therapeutics

et al. 2018

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Comparison of the CMNC and MNC apheresis protocol for the collection of T‐cells showed comparable outcome: An observational study in a single centre

Cited by 13 publications

References 18 publications

Comparison of three mobilization protocols for peripheral blood stem cell apheresis with Spectra Optia continuous mononuclear cell protocol in healthy dogs

Comparison of three mobilization protocols for peripheral blood stem cell apheresis with Spectra Optia continuous mononuclear cell protocol in healthy dogs

Extracorporeal photopheresis in paediatric patients: A retrospective comparison between different ‘off‐line’ protocols

Impact of collection programs for the generation of monocyte apheresis products on product quality and composition as starting material for the generation of cellular therapeutics

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