Comparison of the differentiation potential of neural crest derived progenitor cells from apical papilla (dNC-PCs) and stem cells from exfoliated deciduous teeth (SHED) into mineralising cells

Is There a Role for Neural Crest Stem Cells in Periodontal Regeneration?

“…SCAP express mesenchymal stem cells (MSC)-related cell surface markers, CD29, CD73, CD90, CD105, CD106, CD146, CD166, and STRO-1. SCAP also express periostin and S100A4, and this expression pattern is similar to that of periodontal ligament stem cells [19]. In addition, SCAP express neural crest markers including nestin, musashi-1, p75NTR, snail-1, −2, slug, and Sox9 [21].…”

“…The apical papilla, a soft tissue which is loosely attached to the apex of the developing tooth root, is derived from the neural crest [19]. A population of stem cells has been identified in the root apical papilla of human teeth and termed stem cells from the apical papilla (SCAP) [20].…”

“…These growth factors and known to regulate the biological activities of many types of cells, and thus, may be secreted from SHED and function in an autocrine manner to stimulate their proliferation. The osteoblastic, chondrocytic, adipocytic, and neural cell differentiation potential of SHED was determined after culturing in each induction medium [19,37,41,42]. SHED constitutively expressed vascular endothelial growth factor (VEGF) receptor (VEGFR)-1 and its co-receptor neuropilin-1, and moreover, could differentiate into endothelial cells via exogenous VEGF addition, as identified by the up-regulation of endothelium-related marker expression and the formation of capillary-like sprouts [43].…”

Current and future options of regeneration methods and reconstructive surgery of the facial skeleton

“…SC can either be of embryonic or adult (somatic) origin: embryonic SCs usually divide symmetrically (an embryonic SC often results in two identical embryonic SC) and adult SCs usually divide asymmetrically (an adult SC divides into an SC and a M A N U S C R I P T non-SC) (83). This helps to explain the limited application possibilities of adult SCs in research approaches.…”

Stem Cells from Human Exfoliated Deciduous Teeth: A Growing Literature

“…In comparison with dental neural crest (NC)-derived PC, cells from the apical papilla of retained human third molars, Gosau et al [2013] suggested that dental NC-derived PC are precursors for primary odontoblasts, whereas SHED differentiate into replacement odontoblasts, and these different odontogenic differentiation potentials of dental NC-derived PC and SHED have to be considered for cellular therapies and tissue engineering.…”