Comparison of the direct antiglobulin rosetting reaction with the mixed antiglobulin rosetting reaction for the detection of immunoglobulin on lymphocytes

Fc Receptors on Human Lymphocytes Detected with Double-Coating Indirect Rosette Formation

“…SIg-bearing human blood lymphocytes have been examined by using the mixed an tiglobulin rosetting reaction (MARR) and direct antiglobulin rosetting reaction (DARR) Coombs et al, 1977]: the percentages of B cells in the blood of healthy humans as measured by MARR and DARR were 34 and 31% on average. These figures are substantially higher than the values of B cells determined by DIRF and immunofluorescent studies [Forni and Pernis, 1975;Pape et al, 1977], indicating the difference of the sensitivity among these techniques.…”

Low expression of human histocompatibility leukocyte antigen-DR is associated with hypermethylation of human histocompatibility leukocyte antigen-DR alpha gene regions in B cells from patients with systemic lupus erythematosus.

“…Surface immunoglobulins (Ig) were determined by the conventional immunofluorescene method using fluorescein isothiocyanate-labeled anti-human y, A, or 6 (Dakopatts Lab, Uppsala, Sweden and Behringwerke, Marburg, West Germany), and by the indirect antiglobulin rosetting reaction (28). Quantitative radioimmunoassay for the total IgG and IgM secreted from cells was performed in microplates as followings.…”

Inhibition of Antibody-Dependent Cellular Cytotoxicity and Natural Cytotoxicity by Retinoic Acid

“…Briefly, the non-specific sheep red blood cell (RBC) rosetting reaction was used to deter mine the T lymphocyte population [9], The surface membrane im- munoglobulin (slg)-bearing B lymphocyte population was deter mined by the direct antiglobulin rosetting reaction (DARR) as de scribed by Coombs et al [10]. Fc(IgG) receptor-bearing lympho cytes were determined by opsonic adherence of ox RBC coated with a subagglutinating but optimal dose of rabbit IgG anti-ox-RBC antibody [9].…”