Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients

Perosa, Ana Helena; Watanabe, Aripuanã Sakurada Aranha; Guatura, Sandra Baltazar; Silva, Ellen Ricci Monteiro da; Granato, Celso Francisco Hernandes; Bellei, Nancy

doi:10.6061/clinics/2013(09)05

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…Third, in our experiments we used a LPAIV strain H4N6 which does not lead to clinical signs due to the fact that it establishes low grade infection. Consequently, we resorted to real-time PCR technique to quantify this low grade H4N6 LPAIV infection in the observed tissues, which is more sensitive than the immunofluorescent assay (Landry et al, 2008;Perosa et al, 2013), although immunofluorescent assay would have given more meaningful data relevant to H4N6 LPAIV infection in the observed respiratory and gastrointestinal tissues.…”

Section: Decreasing the Staining Intensity Of Kul01+ Cells Using Clodmentioning

confidence: 99%

Low pathogenic avian influenza virus infection increases the staining intensity of KUL01+ cells including macrophages yet decrease of the staining intensity of KUL01+ cells using clodronate liposomes did not affect the viral genome loads in chickens

Abdul-Cader

Ehiremen

Nagy

et al. 2018

Veterinary Immunology and Immunopathology

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The effect of depletion of macrophages using clodronate liposomes as well as macrophage response following viral infections have been studied in various mouse-virus infection models, but they have not been extensively studied in chickens relevant to virus infections. When we infected day 6 chickens with H4N6 low pathogenic avian influenza virus (LPAIV), we observed that H4N6 LPAIV infection increased the staining intensity of KUL01+ cells in trachea, lungs and duodenum of chickens at 3 days post-infection. Then, we used clodronate liposomes intra-abdominally in 5 day-old chickens and found significant reduction of staining intensity of KUL01+ cells in trachea and duodenum but not in lungs at 4 days post-treatment. When we infected the clodronate liposome and PBS liposome treated chickens with H4N6 LPAIV intra-nasally at day 6, we found no effect on H4N6 LPAIV genome loads in trachea, lungs and duodenum of chickens. This study indicates that although KUL01+ cell intensity are increased in respiratory and gastrointestinal tissues in chickens following H4N6 LPAIV infection, the decrease of KUL01+ cell intensity using clodronate liposomes did not change the H4N6 LPAIV genome loads in any of the examined tissues suggesting that KUL01+ cells may not be critical during H4N6 LPAIV infection in chicken.

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Section: Decreasing the Staining Intensity Of Kul01+ Cells Using Clodmentioning

confidence: 99%

Low pathogenic avian influenza virus infection increases the staining intensity of KUL01+ cells including macrophages yet decrease of the staining intensity of KUL01+ cells using clodronate liposomes did not affect the viral genome loads in chickens

Abdul-Cader

Ehiremen

Nagy

et al. 2018

Veterinary Immunology and Immunopathology

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“…These tests mostly have a specificity greater than 80% and sensitivity of 47-93% depending on virus and viral load. [71,72] These tests are not available for some viruses (e.g., bocavirus, coronavirus, and rhinovirus), and presents lower sensitivity for detection of viruses in co-infections compared with nucleic acid amplification methods.…”

Section: Antigen Detectionmentioning

confidence: 99%

Community-acquired viral pneumonia in human immunodeficiency virus infected patients

Cilloniz

Yamamoto

Rangel

et al. 2014

Community Acquir Infect

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The development of new diagnostic tools has aided better detection of viral pneumonia in the recent years, and viral etiologies have been reported in approximately 23% of pneumonia among inmunocompromised patients. [3,4] However, there are only few studies on RV in patients with human immunodeficiency virus (HIV) infection summarized on Table 1. Human immunodeficiency virus-infected patients present deficiencies in humoral and cell-mediated immunity that can potentially alter the course and severity of common infections. [9] Despite antiretroviral therapy (ART), some HIV patients, especially those with impaired antigen specific responses, may remain at risk for morbidity associated with respiratory viral infections. [9] Additional risk factors for the infections in HIV patients are active smoking and chronic lung comorbidities. The objective of this article is to review the main RV that cause CAP in HIV patients and to discuss their epidemiologies, clinical presentations, diagnosis, and treatments. EPIDEMIOLOGY Approximately, 100 million cases of viral pneumonia are reported in adults annually. [2] Recent studies of CAP in adult population demonstrated that approximately 1-30% of the cases are caused by RV; influenza viruses

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“…Total nucleic acids were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany), according to the manufacturer instructions. The specimens were then tested for the presence of respiratory viruses according to PCR protocols available at our laboratory for influenza A (FLU A) and B (FLU B) viruses [25,26], human respiratory syncytial virus (HRSV) [27], human rhinovirus (HRV) [28], human metapneumovirus (hMPV) [29], human coronavirus (HCoV) [30], human adenovirus (HAdV) [31,32], and parainfluenza virus (PIV 1,2,3,4) [33,34].…”

Section: Methodsmentioning

confidence: 99%

The occurrence of polyomaviruses WUPyV and KIPyV among patients with severe respiratory infections

et al. 2018

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In 2007, the new polyomaviruses WUPyV and KIPyV were identified in patients with acute respiratory infections. The aim of this study was to investigate these viruses in hospitalized patients with severe acute respiratory infection (SARI). A retrospective study was conducted with 251 patients, from April 2009 to November 2010, using nasopharyngeal aspirates, naso-and oropharyngeal swab samples from hospitalized patients (children < 12 years and adults) who had SARI within 7 days of the onset of symptoms, including fever (> 38.8°C), dyspnea, and cough. Clinical and epidemiological information was obtained through standardized questionnaire. Enrolled patients were initially suspected to have influenza A(H1N1)pdm09 infections. WUPyV and KIPyV were detected by real-time PCR. Samples were also tested for influenza A and B viruses, human respiratory syncytial virus, rhinovirus, metapneumovirus, coronavirus, adenovirus, and parainfluenza viruses. WUPyV and KIPyV were detected in 6.77% (4.78% and 1.99%, respectively) of hospitalized patients with SARI. All samples from children showed coinfections (rhinovirus was the most commonly detected). Six adults had polyomavirus infection and four (1.6%) had monoinfection. Of them, 3 reported comorbidities including immunosuppression and 1 patient had worse outcome, requiring ICU admission. These preliminary data may suggest a possible role of polyomaviruses in SARI among immunocompromised adult patients.

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Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients

Cited by 5 publications

References 15 publications

Low pathogenic avian influenza virus infection increases the staining intensity of KUL01+ cells including macrophages yet decrease of the staining intensity of KUL01+ cells using clodronate liposomes did not affect the viral genome loads in chickens

Low pathogenic avian influenza virus infection increases the staining intensity of KUL01+ cells including macrophages yet decrease of the staining intensity of KUL01+ cells using clodronate liposomes did not affect the viral genome loads in chickens

Community-acquired viral pneumonia in human immunodeficiency virus infected patients

The occurrence of polyomaviruses WUPyV and KIPyV among patients with severe respiratory infections

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