Comparison of the effects of Lactobacillus plantarum versus imipenem on infected burn wound healing

Moghadam, Somayeh Soleymanzadeh; Mohammad, Nazanin; Ghooshchian, Maryam; Fathizadeh, Sara; Khodaii, Zohreh; Faramarzi, Mohammad; Aghmiyuni, Zeinab Fagheei; Roudbari, Masoud; Pazouki, Abdolreza; Shabestari, Tahereh Mousavi

doi:10.47176/mjiri.34.94

Cited by 7 publications

(7 citation statements)

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“…Experiments on biofilm eradication have demonstrated how challenging it is to entirely eliminate a biofilm. L. plantarum and its by-products have been shown to possess a high capacity to block the infection-causing abilities of P. aeruginosa and MRSA (Onbas et al, 2019 ; Moghadam et al, 2020 ). Our findings also suggest that Lp2621 has strong biofilm inhibition activity against clinical isolate of MRSA 831 at higher doses ( Figure 3 ).…”

Section: Discussionmentioning

confidence: 99%

“…L. plantarum applied to a wound before and after the initiation of MRSA infection reduced the occurrence of MRSA superinfection in a burn wound model in rats (Sürmeli et al, 2019 ). Previous studies have also shown that probiotics/lactobacilli play a role in healing various types of wounds, such as oral wounds (Han et al, 2019 ), excisional wounds in Wistar rats (Sinha et al, 2019 ), chronic ischemic wound lesions (von Ossowski et al, 2013 ), and burn wounds (Moghadam et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

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Healing wounds, defeating biofilms: Lactiplantibacillus plantarum in tackling MRSA infections

Dubey,

Sharma,

Parul

et al. 2023

Front. Microbiol.

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IntroductionMethicillin-resistant Staphylococcus aureus (MRSA) infections are well-known hospital-borne infections and are a major contributing factor to global health concerns of antimicrobial resistance due to the formation of biofilms. Probiotics are known to assist in the healing of wounds through immunomodulation and also possess anti-pathogen properties via competitive inhibition. The probiotic bacterium, Lactiplantibacillus plantarum MTCC 2621 and its cell-free supernatant (Lp2621) have previously been reported to have antibacterial, excellent antioxidant, and wound healing activity in in vitro conditions and wounds contaminated with S. aureus in mice.MethodsIn the current study, we evaluated its anti-MRSA, biofilm inhibition and eradication efficacy, immunomodulatory activity in THP-1 cells, and wound healing potential in wounds contaminated with MRSA infection in mice.ResultsIn agar well diffusion assay, Lp2621 showed anti-MRSA activity and revealed dose-dependent inhibition and eradication of biofilm by crystal violet assay as well as by Confocal Scanning Laser Microscopy (CLSM) analysis. Further, Lp2621 showed immunomodulatory activity at varied concentrations as measured by IL-6 and IL-10 gene expression in THP-1 cells. Similar findings were observed in serum samples of mice after treatment of excision wound contaminated with MRSA infection by Lp2621 gel, as evident by expression of IL-6 (pro-inflammatory) and IL-10 (anti-inflammatory) cytokines.ConclusionsOverall, our results show that Lp2621 has potent anti-MRSA and antioxidant properties and can prevent and eliminate biofilm formation. It also showed promise when applied to mice with MRSA-infected wounds.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Healing wounds, defeating biofilms: Lactiplantibacillus plantarum in tackling MRSA infections

Dubey,

Sharma,

Parul

et al. 2023

Front. Microbiol.

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“…Specifically, the percentage of wound healing on day 14 was significantly higher in the L. plantarum group compared to the control or imipenem-treated; the mean size of the wound on day 14 was significantly reduced in the probiotic group versus the control group; the wound cultures revealed a significantly increased percentage with no pathogen growth; and the mean leukocyte count was significantly higher in the probiotic group. When viable probiotic cells and supernatant-only treatments were compared, the viable probiotic cells treatment was inferior [32].…”

Section: Lactiplantibacillus Plantarummentioning

confidence: 99%

Looking for the Ideal Probiotic Healing Regime

et al. 2023

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Wound healing is a multi-factorial response to tissue injury, aiming to restore tissue continuity. Numerous recent experimental and clinical studies clearly indicate that probiotics are applied topically to promote the wound-healing process. However, the precise mechanism by which they contribute to healing is not yet clear. Each strain appears to exert a distinctive, even multi-factorial action on different phases of the healing process. Given that a multi-probiotic formula exerts better results than a single strain, the pharmaceutical industry has embarked on a race for the production of a formulation containing a combination of probiotics capable of playing a role in all the phases of the healing process. Hence, the object of this review is to describe what is known to date of the distinctive mechanisms of each of the most studied probiotic strains in order to further facilitate research toward the development of combinations of strains and doses, covering the whole spectrum of healing. Eleven probiotic species have been analyzed, the only criterion of inclusion being a minimum of two published research articles.

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“…Isso por conta do seu potencial imunomodulador e antimicrobiano, indicando que o uso de L. plantarum pode ser uma nova alternativa adjuvante ao uso de antibióticos. 29 Lactobacillus gasseri também apresentou efeito contra contaminação/infecção ocasionada por P. aeruginosa. O sobrenadante da cepa de L. gasseri 63 AM inibiu o desenvolvimento de biofilmes e ainda eliminou os que estavam pré-desenvolvidos, usando a metodologia em microplaca de 24 poços e infectando os modelos murinos com P. aeruginosa no local da ferida, observou-se que a cepa de L. gasseri 63 AM preveniu a ocorrência de sepse.…”

Section: Lactobacillus Spp -Potencial Terapêuticounclassified

Lactobacillus spp. na vanguarda: abordagem terapêutica não-medicamentosa no combate efetivo à Pseudomonas aeruginosa

Rodrigues da Silva,

Torquati,

Araujo

et al. 2024

RUC

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Introdução: A resistência antimicrobiana representa um alarmante problema de saúde global, sendo o uso indiscriminado de antibióticos o principal contribuinte para esse cenário. Assim, a busca por terapias que contornem as resistências está entre os estudos contemporâneos mais necessários. Dentre os patógenos humanos com elevada resistência se destaca a bactéria gram-negativa Pseudomonas aeruginosa. Por outro lado, uma abordagem não medicamentosa promissora, os probióticos, um grupo de bactérias não patogênicas, quando presentes em proporções adequadas, resultam em efeitos benéficos ao hospedeiro. Dentre os probióticos o gênero Lactobacillus é o mais estudado, apresentam potencial efeito imunomodulador e antimicrobiano. Objetivo: Compilar as principais descobertas sobre o efeito de Lactobacillus spp. sobre o crescimento de patógenos. Método: Trata-se de uma revisão sistemática baseada na metodologia PRISMA, com critérios de exclusão e elegibilidade, abrangendo os últimos 8 anos. Um total de 21 artigos foram incluídos para análise. Resultados: Diferentes estudos mencionam o uso de probióticos com efeitos benéficos no hospedeiro e na homeostase da microbiota. Estudos recentes indicam que, além dos probióticos, os produtos desses micro-organismos, chamados de pós-bióticos, poderiam ser uma promissora abordagem terapêutica para controlar o crescimento e/ou a colonização por P. aeruginosa. Conclusão: Os probióticos e seus produtos embora se apresentem como seguros para uso em humanos, parecem ter seus efeitos benéficos espécie-específicos, particularmente no tocante ao controle de crescimento e/ou colonização por P. aeruginosa. Probióticos e pós-bióticos poderiam ser empregados como complementos às abordagens clínicas para o combate de infecções bacterianas, especialmente da espécie P. aeruginosa.

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Comparison of the effects of Lactobacillus plantarum versus imipenem on infected burn wound healing

Cited by 7 publications

References 0 publications

Healing wounds, defeating biofilms: Lactiplantibacillus plantarum in tackling MRSA infections

Healing wounds, defeating biofilms: Lactiplantibacillus plantarum in tackling MRSA infections

Looking for the Ideal Probiotic Healing Regime

Lactobacillus spp. na vanguarda: abordagem terapêutica não-medicamentosa no combate efetivo à Pseudomonas aeruginosa

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