Comparison of the effects of zinc-silver cream and Nigella sativa oil on wound healing and oxidative stress in the wound model in rats

Abstract

The present study was undertaken to evaluate of Nigella sativa oil (NSO) and zinc-silver cream in a rat model of wound healing by means of clinical appearance, histopathology, oxidative stress parameters, and biomechanical tests. The rats were divided into three groups, each having twenty animals. Group 1 served as a control having wound. Other two groups received either NS oil or zinc-silver cream in addition to the wound. The study was initiated after the animals rested for 2 weeks for acclimation. Blood samples were collected from 10 randomly selected animals from each group at days 0, 3, 7 and 14 for oxidative stress measurements. Differences among groups for blood lipid peroxidation and antioxidant values were determined with respect to biochemical oxidative stress results (P<0.01). Wound sites of 10 animals from each group were allocated for histopathological examinations, and those of the remaining 10 animals from each group were used in biomechanical testing. Histopathological examination revealed that epithelization occurred in all groups. Inflammatory response with an intensive vascularization was present in the samples of the silver group (P<0.01). Consequently, zinc-silver group has differed positively in terms of biochemical and biomechanical and histological aspects compared to other groups for wound healing and oxidative stress.

Nigella sativa and Its Active Compound, Thymoquinone, Accelerate Wound Healing in an In Vivo Animal Model: A Comprehensive Review

“…All articles were published between the years 2010 and 2020. There were four types of wound investigated among the studies, namely excision wound [14,[40][41][42], diabetic ulcers [43][44][45], deep second-degree burn [46,47], and chemical burn [48].…”

“…According to wound depth, three types of wound depths were created, which were superficial partial-thickness wound [40], deep partial-thickness wound [47,48], and full-thickness wound [43,45,46]. The remaining articles [14,41,42,44] did not specify any details about the depth of wound in their studies.…”

“…Three studies used NS oil extract in which NS seeds were cold pressed to produce oil in one study [40], while the remaining two studies did not explain the process [14,48]. Four studies demonstrated NS oil cream [41,42,45,47] as their topical approach and one study demonstrated NS oil gel [43]. To fabricate NS oil cream, three studies used fixed oil [42,45,47] while the other one did not have the information [37].…”

“…Four studies demonstrated NS oil cream [41,42,45,47] as their topical approach and one study demonstrated NS oil gel [43]. To fabricate NS oil cream, three studies used fixed oil [42,45,47] while the other one did not have the information [37]. TQ was used in the two remaining studies in two forms; 10% in petroleum jelly (w/v) [44] and 0.5% solution, prepared by dissolving 500 mg TQ in 100 mL dimethyl sulphoxide (DMSO) [46].…”

“…According to geographical origin, two studies purchased their NS oil from Iran [40,45], three studies from Turkey [41,42,47], and another study from Indonesia [43] and India [14] each. Following that, one study obtained NS oil extracted by Aljouf University, Saudi Arabia [48].…”