Comparison of the Effects of Different Strains of <i>Lactobacillusin</i> Reducing Bacterial Translocation on Methotrexate-lnduced Enterocolitis in Rats

Abstract

Certain strains of Lactobacillus, which have therapeutic and nutritional value, may facilitate the reduction of bacterial translocation in hosts under critical conditions. The effects may vary between different strains of Lactobacillus. The aim of this study was to evaluate and compare the impact of three Lactobacillus strains on methotrexate-induced enterocolitis in rats. From the beginning of the study, rats received an intragastric infusion of an elemental diet alone or supplemented with Lactobacillus plantarum DSM 6595, Lactobacillus rhamnosus DSM 6594, or Lactobacillus acidophilus DSM 20079 in fermented oatbase. Methotrexate (20 mg/kg) was injected intraperitoneally to all animals on the 4th day, and sampling was done on the 7th day. Supplementation of all three Lactobacillus strains significantly lowered the bacterial translocation to mesenteric lymph nodes, liver and blood, which was accompanied by a reduction in overgrown pathogenic bacteria and an increase in lactobacilli in rats with enterocolitis. L. plantarum and L. rhamnosus were more effective in reducing the bacterial translocation and improving the gut mucosal protein, DNA and RNA content than L. acidophilus. The present results may imply that L. plantarum and L. rhamnosus have more significance than L. acidophilus in future bacteriotherapy.

Lactobacillus plantarum 299v Does Not Reduce Enteric Bacteria or Bacterial Translocation in Patients Undergoing Colon Resection

“…In experimental studies, probiotics have been shown to attenuate BT [2][3][4]. BT occurs in humans [15] and its relation to sepsis is presumed but not proven.…”

“…This is one reason why probiotic bacteria, i.e., living microorganisms with beneficial effects on the host [1], have gained increased interest in the last decade. Probiotics have been found to reduce BT in animal experiments [2][3][4]. However, clinical studies have been controversial.…”

Probiotic Bacillus amyloliquefaciens SC06 Prevents Bacterial Translocation in Weaned Mice

“…*P \ 0.05 (t test) lactobacilli either orally or by enema will further reduce the translocation [1]. The effect is different for different lactobacilli and the strongest effect was found with Lactobacillus plantarum and Lactobacillus rhamnosus [15]. The mechanisms underlying the decreased translocation are not illustrated.…”

Adhesive capability of Lactobacillus plantarum 299v is important for preventing bacterial translocation in endotoxemic rats

“…Supplementation with probiotics has proven effective in preventing pancreatic sepsis (5) and postoperative infection in liver transplant patients (6). It has previously been shown that a specific probiotic strain, Lactobacillus plantarum 299v, can reduce intestinal permeability induced by Escherichia coli (7) and abate bacterial translocation in methotrexate‐induced enterocolitis (8) and liver injury (9).…”