Probiotic Bacillus amyloliquefaciens SC06 Prevents Bacterial Translocation in Weaned Mice

Abstract

Probiotic is a preparation containing microorganisms that confers beneficial effect to the host. This work assessed whether oral administration of Bacillus amyloliquefaciens SC06 (Ba) could decrease bacterial translocation in weaned mice. Weaned C57BL/6 were randomly allocated into three groups: group I as the control group, group II were treated with 0.85 % NaCl. Group III was administered with probiotic Ba 1 9 10 9 CFU/day dissolved in 100 ll of 0.85 % NaCl for 30 days. Mice were then sacrificed, and tissue were cultured to determine bacterial translocation. Meanwhile, splenic CD4 ? T cells, CD8 ? T cells, B cells, and macrophages were analysised by FACS. Our results showed that probiotic Ba significantly reduced bacteria translocation compared with the control group and 0.85 % NaCl group (P \ 0.05), lower levels of bacteria were detected in the MLN, liver, spleen, and kidney of mice. Moreover, significant increase in percentage and number of macrophages were observed in the spleen of Ba-treated mice compared with the control and 0.85 % NaCl groups. Together, these data indicated that Ba could decrease bacterial translocation in weaned mice. This effect seems to be correlated with the changes of macrophage numbers.

Effects of Bacillus amyloliquefaciens TL106 Isolated from Tibetan Pigs on Probiotic Potential and Intestinal Microbes in Weaned Piglets

“…Lei et al confirmed that the addition of different doses of Bacillus amyloliquefaciens in the diet significantly improved the overall growth performance and morphology of small intestine and increased the number of lactobacilli in the cecum ( 41 ). Ji, Hu, and Li reported that Bacillus amyloliquefaciens SC06 significantly increased the daily weight gain and reduced the diarrhea rate of weaned piglets in vivo ( 26 ). In our study, TL106 and antibiotics significantly increased the bodyweight of piglets at 7 and 14 days after weaning.…”

“…Yongtao brings to light that the addition of B. amyloliquefaciens probiotics to the prenatal diet of pregnant sows promoted the production of short-chain fatty acids (SCFAs) in the intestines of both sows and newborn piglets with the improvement of their intestinal flora ( 24 ). Moreover, the oral administration of B. amyloliquefaciens SC06 can reduce bacterial translocation and affect the intestinal immune function in weaned mice ( 25 , 26 ).…”

In Vitro and In Vivo Anti-Clostridioides difficile Effect of a Probiotic Bacillus amyloliquefaciens Strain

“…in a broiler CDI model where B. amyloliquefaciens did not confer protection to the in vivo model but showed significant anti-CD activity in in vitro experiments [ 56 ]. However, several previous studies reported that B. amyloliquefaciens successfully conferred protection against C. Difficile-associated disease in a mouse model [ 57 - 59 ]. The difference in physiology and infection surface area between hamsters and mice might be the reason for the observed variation in B. amyloliquefaciens efficacy, as was previously proposed for auranofin [ 60 ].…”

Bacillus amyloliquefaciens SC06 alleviates the obesity of ob/ob mice and improves their intestinal microbiota and bile acid metabolism

“…Thus, Bacillus amyloliquefaciens can be used as a probiotic in animal-healthy production. 24 Our previous work implied that Bacillus amyloliquefaciens SC06 (SC06) protected mice from HFD-induced obesity and liver injury via regulating the host metabolism and intestinal microbiota. 25 Hence, in order to investigate the mechanisms by which Bacillus reduces obesity in more detail, we explored the role of the probiotic Bacillus amyloliquefaciens SC06 in reshaping the intestinal microbial composition and changing metabolites and focused on the effects on bile acid metabolism in male ob/ob C57BL/6J mice as genetic obesity models.…”