Comparison of the efficiencies of true blue and diamidino yellow as retrograde tracers in the peripheral motor system

Haase, Peter; Payne, J.N.

doi:10.1016/0165-0270(90)90107-q

Cited by 20 publications

(18 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although there was a tendency for the counts at 28 days to be lower than at earlier time points, this was not statistically significant. The occasional occurrence of motoneurones labelled with fast blue alone agrees with previous reports [13,16] and is probably due to fast blue dispersing more widely than diamidino yellow at the site of injection [17].…”

Section: Methodological Considerationssupporting

confidence: 80%

Time Course of Motoneurone Death after Neonatal Sciatic Nerve Crush in the Rat

Lowrie

Lavalette

Davies³

1994

Dev Neurosci

View full text Add to dashboard Cite

Sciatic motoneurones were retrogradely labelled with long-lasting fluorescent dyes prior to unilateral nerve crush in either 3-day-old or adult rats. The number of surviving labelled motoneurones at intervals after nerve injury were compared to the number in the contralateral control ventral horn and in unoperated animals. Following adult nerve crush there was no significant reduction in the number of labelled motoneurones, but after neonatal nerve crush the count was reduced to about 35%. Most of the cell death occurred during the first 6 days after nerve injury, mainly from the lower half of the motor column, but about one third died between 6 and 12 days, mainly from the upper part. These results suggest that less mature motoneurones tend to die earliest, before the muscle is reinnervated. Those in the upper, more mature part of the motor pool survive longer but may still die during reinnervation. At least two types of glial cell were secondarily labelled by this method, distinguished by their response to nerve injury.

show abstract

Section: Methodological Considerationssupporting

confidence: 80%

Time Course of Motoneurone Death after Neonatal Sciatic Nerve Crush in the Rat

Lowrie

Lavalette

Davies³

1994

Dev Neurosci

View full text Add to dashboard Cite

show abstract

“…We used a concentration of 2% FG in the double labelling experiments, as preliminary experiments showed that the use of this concentration in combination with 5% FB yielded similar mean numbers of labelled neurones. For DY, we decided to use 5% rather than 2% which is the concentration used by many other investigators (Keizer et al, 1983;Innocenti et al, 1986, Craig et al, 1989) because 2% DY has been reported to have a lower tracer efficiency than 3% FB (Horikawa et al, 1986) or 2% True Blue (Haase and Payne, 1990).…”

Section: Concentration and Amount Of Tracer Injectedmentioning

confidence: 99%

“…Blue and Diamidino Yellow when tested by different ways of tracer administration to sciatic nerve motoneuron axons in the rat (Haase and Payne, 1990). Fluorescent dyes have also been used to retrogradely label dorsal root ganglia (DRG) cell bodies after injection directly in peripheral tissues such as muscle (Illert et al, 1982;O'Brien, 1989;Buisseret-Delmas, 1990;Richmond et al, 1994), or skin (O'Brien, 1989, but there is little information regarding the relative efficiency of different fluorescent retrograde tracers after cutaneous injection.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection

Puigdellı́vol-Sánchez

Prats-Galino

Ruano-Gil

et al. 1998

Journal of Neuroscience Methods

View full text Add to dashboard Cite

“…We used True Blue dye, which reliably labels only DRG neurons after peripheral injection [10]. We used subcutaneous injection for labeling neurons innervating the plantar aspect of the foot and a subepineurial microinjection technique for labeling sciaticprojecting neurons.…”

Section: Drg Neuronal Somata Projecting To Paw and Distal Sciatic Nervementioning

confidence: 99%

Species and strain differences in rodent sciatic nerve anatomy: Implications for studies of neuropathic pain

Rigaud

Gemes

Barabas

et al. 2008

Pain

254

235

View full text Add to dashboard Cite

Hindlimb pain models developed in rats have been transposed to mice, but assumed sciatic nerve neuroanatomic similarities have not been examined. We compared sciatic nerve structural organization in mouse strains (C57BL/6J, DBA/2J, and B6129PF2/J) and rat strains (Wistar, Brown Norway, and Sprague-Dawley). Dissection and retrograde labeling showed mouse sciatic nerve origins predominantly from the third lumbar (L3) and L4 spinal nerves, unlike the L4 and L5 in rats. Proportionate contributions by each level differed significantly between strains in both mice and rats. Whereas all rats had six lumbar vertebrae, variable patterns in mice included mostly five vertebrae in DBA/2J, mostly six vertebrae in C57BL/6J, and a mix in B6129PF2/J. Mice with a short lumbar vertebral column showed a rostral shift in relative contributions to the sciatic nerve by L3 and L4. Ligation of the mouse L4 nerve created hyperalgesia similar to that in rats after L5 ligation, and motor changes were similar after mouse L4 and rat L5 ligation (foot cupping) and after mouse L3 and rat L4 ligation (flexion weakness). Thus, mouse L3 and L4 neural segments are anatomically and functionally homologous with rat L4 and L5 segments. Neuronal changes after distal injury or inflammation should be sought in the mouse L3 and L4 ganglia, and the spinal nerve ligation model in mice should involve ligation of the L4 nerve while L3 remains intact. Strain-dependent variability in segmental contributions to the sciatic nerve may account in part for genetic differences in pain behavior after spinal nerve ligation.

show abstract

Comparison of the efficiencies of true blue and diamidino yellow as retrograde tracers in the peripheral motor system

Cited by 20 publications

References 25 publications

Time Course of Motoneurone Death after Neonatal Sciatic Nerve Crush in the Rat

Time Course of Motoneurone Death after Neonatal Sciatic Nerve Crush in the Rat

Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection

Species and strain differences in rodent sciatic nerve anatomy: Implications for studies of neuropathic pain

Contact Info

Product

Resources

About