Comparison of the genetic maps of variola and vaccinia viruses

Щелкунов, С. Н.; Resenchuk, Sergei; Av, Totmenin; Блинов, В. М.; Маренникова, С. С.; Sandakhchiev, L. S.

doi:10.1016/0014-5793(93)81013-p

Cited by 54 publications

(39 citation statements)

References 12 publications

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“…For comparisons we included sequences obtained from three strains of variola virus -India (Shchelkunov et al, 1993), Harvey and Somalia (Douglass & Dumbell, 1992); two additional strains of vaccinia virusCopenhagen (Goebel et al, 1990) and WR (GenBank accession no. M35027).…”

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confidence: 99%

DNA sequence variation as a clue to the phylogenesis of orthopoxviruses

Douglass

Dumbell

1996

Journal of General Virology

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We have sequenced DNA equivalent to the E5R ORF of Copenhagen vaccinia virus from an additional strain of vaccinia and from cowpox (three strains), camelpox (two strains), taterapox and ectromelia viruses. None of these showed the disruptions previously reported in the equivalent region of monkeypox virus. We also constructed a viable recombinant of vaccinia virus strain Dairen in which the E5R sequence was disrupted by a 436 bp deletion and substitution of the E. coli gpt gene. Quantitative analysis of the sequences, including available sequences from monkeypox, variola and vaccinia viruses revealed four main groupings, namely cowpox, ectromelia, monkeypox and a cluster which includes variola, camelpox, taterapox and vaccinia viruses. It was noted that, at over 75% of the positions which differentiated species, all species but one had a common nucleotide. Although the analysis covers one single gene only, the results accord with what is known of the biology of the viruses.

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confidence: 99%

DNA sequence variation as a clue to the phylogenesis of orthopoxviruses

Douglass

Dumbell

1996

Journal of General Virology

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“…The probe locations relative to the sequence of the vTNFR gene of the variola virus (EMBL accession no. X69198 [45,46]) are shown in Fig. 1.…”

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confidence: 99%

Species-Level Identification of Orthopoxviruses with an Oligonucleotide Microchip

et al. 2002

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A method for species-specific detection of orthopoxviruses pathogenic for humans and animals is described. The method is based on hybridization of a fluorescently labeled amplified DNA specimen with the oligonucleotide DNA probes immobilized on a microchip (MAGIChip). The probes identify species-specific sites within the crmB gene encoding the viral analogue of tumor necrosis factor receptor, one of the most important determinants of pathogenicity in this genus of viruses. The diagnostic procedure takes 6 h and does not require any sophisticated equipment (a portable fluorescence reader can be used).

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“…It was shown [3] that the CPV gene coding for the 77 kDa protein is responsible for this property of the virus and when integrated into the VAC genome allows the latter virus to propagate in CHO cells. Another gene which is necessary for VAC Earlier we cloned the fragments of DNA of VAR strain India-1967 in molecular vectors of E. coli [6]. The Maxam and Gilbert technique [7] was used to determine the full coding sequence of this virus genome; it contains 193 open reading frames (ORF).…”

Section: Methodsmentioning

confidence: 99%

Ankyrin‐like proteins of variola and vaccinia viruses

1993

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Computer analysis of full coding sequences of variola major virus strain India‐1967 genome and vaccinia virus strain Copenhagen genome have been carried out. A wide set of proteins containing ankyrin‐like repeats have been identified for both viruses. Only three proteins of this family of the studied viruses are highly homologous. The rest of the proteins are different. The possible role of such proteins in determination of virus tissue tropism is discussed.

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Comparison of the genetic maps of variola and vaccinia viruses

Cited by 54 publications

References 12 publications

DNA sequence variation as a clue to the phylogenesis of orthopoxviruses

DNA sequence variation as a clue to the phylogenesis of orthopoxviruses

Species-Level Identification of Orthopoxviruses with an Oligonucleotide Microchip

Ankyrin‐like proteins of variola and vaccinia viruses

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