We now realize that individual values, obtained while observing apparently healthy animals, may deviate appreciably from the mean of such values and still, so far as we can tell, be compatible with health. Values that deviate considerably from the mean may nevertheless be 'normal'. Values for the concentration of haemoglobin in the blood belong to this category and pose rather special problems of their own. Here we are dealing with two variables, the mass of the haemoglobin and the volume of the plasma, and these may vary independently. The reading obtained in conventional haemoglobinometry is a function of both. Whitby (195 I) discusses this problem fully.The accepted 'normal' value for the concentration of haemoglobin in human blood has increased progressively with the passage of years. Whereas 13.8 g/Ioo ml. blood was ' IOO % ' for Haldane's original twelve healthy men, something approaching 16 g/Ioo ml. blood is now thought to be the desirable concentration in men. But the concentration in the blood of apparently healthy adult women, even when all factors other than sex are comparable, has obstinately lagged behind, so that something of the order of 14 g haemoglobin/Ioo ml. blood is accepted as normal for them ( Until the age of puberty the concentration of haemoglobin in the blood of healthy boys and girls is the same (Williamson, 1916). From puberty to menopause the 'normal' concentration of haemoglobin in the blood of apparently healthy women is less than the 'normal' concentration in the blood of men. After the menopause the values for women approximate to, but never quite reach, the values for men of the same age. Adult civilized men and women form two distinct 'populations', in a statistical sense, with respect to the concentration of haemoglobin in their blood, although there is very considerable overlap. It should be noted, however, that histograms of the values for women in general are skew, owing to a large number of low values (Milam & Muench, 1946). Davies, Levin & Oberholzer (1952) have recently pointed out that the concentration of iron in the serum of women is lower than that in the serum of men, although the iron-binding capacity of the serum is the same. Whatever the explanation, these observed differences between men and women are undoubtedly associated with the period of female sexual activity.
1952).