Comparison of the ICSI outcome of ejaculated sperm with normal, abnormal parameters and testicular sperm

Göker, Ege Nazan Tavmergen; Şendağ, Fatih; Levi, Rafael; Sendag, H.; Tavmergen, Erol

doi:10.1016/s0301-2115(02)00067-2

Cited by 33 publications

(25 citation statements)

References 32 publications

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“…Low fertilization, cleavage and embryo quality rates after ICSI have also been found by other groups (4,(6)(7)(8), who compared the same parameters utilizing sperm from different sources: normal or abnormal ejaculated sperm, sperm from OA patients and sperm from NOA patients. Slower and lower blastocyst formation rates, after extended in vitro culture of embryos produced by ICSI, have been demonstrated in cases of epididymal sperm extracted from OA patients or testicular sperm extracted from NOA patients, as compared to the control group (5,9).…”

Section: Discussionsupporting

confidence: 63%

“…In addition, blastocyst formation rates were shown to be lower after ICSI than after IVF (5). Embryo development, pregnancy and implantation rates have been found to be affected by the source of sperm (ejaculated, epididymal, or testicular) and the type of male factor infertility (OA or NOA) (6). ICSI using testicular sperm has been found to produce lower fertilization and pregnancy rates than normal ejaculated spermatozoa (6).…”

Section: Introductionmentioning

confidence: 99%

“…Embryo development, pregnancy and implantation rates have been found to be affected by the source of sperm (ejaculated, epididymal, or testicular) and the type of male factor infertility (OA or NOA) (6). ICSI using testicular sperm has been found to produce lower fertilization and pregnancy rates than normal ejaculated spermatozoa (6). Finally, fertilization, embryo development, blastocyst formation, pregnancy and implantation rates after ICSI are significantly lower in NOA patients in comparison to OA patients (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

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The effects of sperm quality on embryo development after intracytoplasmic sperm injection

Loutradi

Tarlatzis

Goulis

et al. 2006

J Assist Reprod Genet

122

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Purpose:To explore the possible relationship between sperm quality and embryo development, pregnancy and implantation rates, in patients undergoing intracytoplasmic sperm injection (ICSI). Methods: Fertilization and cleavage rates, quality of embryos, blastocyst development, pregnancy and implantation rates were analyzed in 1020 embryos from 219 couples undergoing first ICSI treatment cycle. The couples were allocated in five groups, according to semen parameters: Group 1: patients with normal semen parameters, Group 2: patients with mild oligoastheno-teratozoospermia, Group 3: patients with severe oligo-astheno-teratozoospermia, Group 4: patients with obstructive azoospermia, Group 5: patients with non-obstructive azoospermia. Results: Fertilization and cleavage rates, quality of embryos as well as blastocyst development rates were significantly reduced, as semen quality decreased. However, no significant differences were observed in clinical pregnancy and implantation rates. Conclusion: Overall, a negative relationship was observed between semen quality and embryo development, even before activation of the embryonic genome, suggesting that sperm can affect embryogenesis from a very early stage.

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Section: Discussionsupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effects of sperm quality on embryo development after intracytoplasmic sperm injection

Loutradi

Tarlatzis

Goulis

et al. 2006

J Assist Reprod Genet

122

View full text Add to dashboard Cite

show abstract

“…Most studies evaluating ICSI and azoospermia take into account only the sperm source but not the type of azoospermia. These studies tend to have a better outcome when epididymal spermatozoa are used, but these findings can be justified by the fact that epididymal sperm are always from obstructive azoospermia, while testicular sperm can be from both types of azoospermia (20,22). Apart from very few studies, there is no systematic differentiation between obstructive and nonobstructive azoospermia.…”

Section: Commentsmentioning

confidence: 99%

Sperm defect severity rather than sperm source is associated with lower fertilization rates after intracytoplasmic sperm injection

Verza¹,

Esteves²

2008

Int. braz j urol.

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Objective: To evaluate the impact of sperm defect severity and the type of azoospermia on the outcomes of intracytoplasmic sperm injection (ICSI). Materials and Methods:This study included 313 ICSI cycles that were divided into two major groups according to the source of spermatozoa used for ICSI: 1) Ejaculated (group 1; n = 220) and 2) Testicular/Epididymal (group 2; n = 93). Group 1 was subdivided into four subgroups according to the results of the semen analysis:, n = 41), 2) double defect (a combination of two single defects, n = 45), 3) triple defect (OAT, n = 48), and 4) control (no sperm defects; n = 86). Group 2 was subdivided according to the type of azoospermia: 1) obstructive (OA: n = 39) and 2) non-obstructive (NOA: n = 54). Fertilization (2PN), cleavage, embryo quality, clinical pregnancy and miscarriage rates were statistically compared using one-way ANOVA and Chi-square analyses. Results: Significantly lower fertilization rates were obtained when either ejaculated sperm with triple defect or testicular sperm from NOA patients (63.4 ± 25.9% and 52.2 ± 29.3%, respectively) were used for ICSI as compared to other groups (~73%; P < 0.05). Epididymal and testicular spermatozoa from OA patients fertilized as well as normal or mild/moderate deficient ejaculated sperm. Cleavage, embryo quality, pregnancy and miscarriage rates did not differ statistically between ejaculated and obstructive azoospermia groups. However, fertilization, cleavage and pregnancy rates were significantly lower for NOA patients. Conclusion: Lower fertilization rates are achieved when ICSI is performed with sperm from men with oligoasthenoteratozoospermic and non-obstructive azoospermic, and embryo development and pregnancy rates are significantly lower when testicular spermatozoa from NOA men are used.

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“…Goker et al evaluated cycles based on sperm quality and source, comparing ejaculated mobile sperm to ejaculated immobile sperm to testicular sperm. He found that regardless of sperm quality the ejaculated sperm performed better than the testicular sperm with increased fertilization rates, rates of grade I embryos and pregnancy rates [14]. A recent published abstract of a randomized prospective trial, documented that percutaneous epididymal aspiration improves embryo development and pregnancy rates when compared to TESA, also suggesting a benefit when using further matured sperm [9].…”

mentioning

confidence: 99%

Fresh MESA improved embryo fertilization, cleavage, blastula formation and implantation rates after failed TESA in couples with obstructive azoospermia.

Keltz

Rovner

González

et al. 2011

J Assist Reprod Genet

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Comparison of the ICSI outcome of ejaculated sperm with normal, abnormal parameters and testicular sperm

Cited by 33 publications

References 32 publications

The effects of sperm quality on embryo development after intracytoplasmic sperm injection

The effects of sperm quality on embryo development after intracytoplasmic sperm injection

Sperm defect severity rather than sperm source is associated with lower fertilization rates after intracytoplasmic sperm injection

Fresh MESA improved embryo fertilization, cleavage, blastula formation and implantation rates after failed TESA in couples with obstructive azoospermia.

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