Comparison of the immunocytochemical distribution of the phosphoprotein B-50 in the cerebellum and hippocampus of immature and adult rat brain

Oestreicher, A.B.; Gispen, W.H.

doi:10.1016/0006-8993(86)90747-x

Cited by 186 publications

(87 citation statements)

References 28 publications

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“…In our wild-type cerebella, B-50/GAP-43 immunolabeling faithf ully reproduced previously reported distribution patterns (Oestreicher and Gispen, 1986;Baürle et al, 1994): the molecular layer displayed an intense punctate staining, whereas all Purkinje cell perikarya were virtually negative (Fig. 2 A,B).…”

Section: B-50/gap-43 Expression In Intact and Injured Wild-type Mousesupporting

confidence: 91%

“…Uninjured Purkinje cells do not show B-50/GAP-43 expression either during development (Oestreicher and Gispen, 1986;Console-Bram et al, 1996) or in adulthood (Oestreicher and Gispen, 1986;Meberg and Routtenberg, 1991;Kruger et al, 1993;Baürle et al, 1994;Console-Bram et al, 1996). However, it is not known whether B-50/GAP-43 can be upregulated in adult Purkinje cells after axon injury or transplantation of growthpromoting tissues.…”

Section: B-50/gap-43 Expression In Intact and Injured Wild-type Mousementioning

confidence: 99%

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Targeted Overexpression of the Neurite Growth-Associated Protein B-50/GAP-43 in Cerebellar Purkinje Cells Induces Sprouting after Axotomy But Not Axon Regeneration into Growth-Permissive Transplants

et al. 1997

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B-50/GAP-43 is a nervous tissue-specific protein, the expression of which is associated with axon growth and regeneration. Its overexpression in transgenic mice produces spontaneous axonal sprouting and enhances induced remodeling in several neuron populations (Aigner et al., 1995;Holtmaat et al., 1995). We examined the capacity of this protein to increase the regenerative potential of injured adult central axons, by inducing targeted B-50/GAP-43 overexpression in Purkinje cells, which normally show poor regenerative capabilities. Thus, transgenic mice were produced in which B-50/GAP-43 overexpression was driven by the Purkinje cell-specific L7 promoter. Uninjured transgenic Purkinje cells displayed normal morphology, indicating that transgene expression does not modify the normal phenotype of these neurons. By contrast, after axotomy numerous transgenic Purkinje cells exhibited profuse sprouting along the axon and at its severed end. Nevertheless, despite these growth phenomena, which never occurred in wild-type mice, the severed transgenic axons were not able to regenerate, either spontaneously or into embryonic neural or Schwann cell grafts placed into the lesion site. Finally, although only a moderate Purkinje cell loss occurred in wild-type cerebella after axotomy, a considerable number of injured transgenic neurons degenerated, but they could be partially rescued by the different transplants placed into the lesion site. Thus, B-50/GAP-43 overexpression substantially modifies Purkinje cell response to axotomy, by inducing growth processes and decreasing their resistance to injury. However, the presence of this protein is not sufficient to enable these neurons to accomplish a full program of axon regeneration.

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Section: B-50/gap-43 Expression In Intact and Injured Wild-type Mousesupporting

confidence: 91%

Section: B-50/gap-43 Expression In Intact and Injured Wild-type Mousementioning

confidence: 99%

Targeted Overexpression of the Neurite Growth-Associated Protein B-50/GAP-43 in Cerebellar Purkinje Cells Induces Sprouting after Axotomy But Not Axon Regeneration into Growth-Permissive Transplants

et al. 1997

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“…The distribution of GAP-43 in the septal and mid-septotemporal dentate gyrus corresponds to that of the rat (Benowitz et al, 1988;Oestreicher and Gispen, 1986). There are no other descriptions of changes in the distribution of GAP-43 along the septotemporal axis of the dentate gyrus.…”

Section: Gap-43mentioning

confidence: 86%

A morphologically distinct granule cell type in the dentate gyrus of the red fox correlates with adult hippocampal neurogenesis

Amrein¹,

Slomianka²

2010

Brain Research

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A morphologically distinct granule cell type in the dentate gyrus of the red fox correlates with adult hippocampal neurogenesis Running titleThe dentate gyrus of the red fox Text pages 37Figures 7 Tables 3 Corresponding authorIrmgard In summary, we report a morphologically distinct granule cell type which correlates with adult 3 hippocampal neurogenesis in the fox. Furthermore, the maturation phase of the young neurons may be prolonged as in other long-living species such as primates. SECTIONNervous System Development, Regeneration and Aging

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“…The specificity of the antibodies to ,CGRP (5) and B-50 (10,11,39) has been tested previously. The specificity of the antisera was determined with use of the conven tional method of substituting normal rabbit preimmune or mouse serum for the primary antiserum and omitting the primary antise rum step.…”

Section: Iniinunocytocheniistrymentioning

confidence: 99%

“…This antibody has been applied to detect vascularization or bone remodeling, and pain and pro prioception (2, 3,19,26), outgrowing nerve fibres (48,52,53). A high concen tration of B-50/GAP-43 has been found in axonal The present study focussed on the changes in the growth cones during embryonic (37,50) and post innervation pattern of sensory fibres associated with natal development (10,11,39). The expression of Bbone remodeling after insertion of an implant.…”

mentioning

confidence: 99%

Changes in innervation of long bones after insertion of an implant: Immunocytochemical study in goats with antibodies to calcitonin gene‐related peptide and B‐50/GAP‐43

Buma¹,

Elmans²,

Oestreicher

1995

Journal Orthopaedic Research

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Sum m ary: In this study, we describe the distribution of fibres th a t co n tain calcitonin g e n e -re la te d p e p tid e in norm al bones and in bones th a t are rem odeling a fter insertion of an im plant. W ith ro u tin e histology and a n ti bodies to calcitonin gen e-related peptide, sm all neural and free-ru n n in g fibres staining positively lo r c alcito nin gene-related p ep tid e w ere found in the p erio steu m , en d o steu m , and cortical bone of the tibia in the goat. In m any cases, th e free-running fibres w ere associated with b lood vessels th a t e n te re d the b o n e th ro u g h V olkm a n n 's canals. T he endosteal b lood supply was d estro y ed as a result of in sertio n of th e im plant. T h e n ecro tic b o n e was no longer innervated, as show n by th e lack of staining for th e antibodies. A t 6 w eeks, a re p a ir p h ase sta rte d with revascularization and rem o d elin g of the necrotic en d o steal bone. D u rin g this re p a ir phase, th e re was increased innervation with fibres containing calcitonin g en e-related p e p tid e in the rem o d elin g cavities at the interface betw een living and necrotic bone, T hese fibres en d ed blindly, w ith m any large varicosities, an d could be d em o n strated by im m unostaining with m onoclonal antibodies to B -50/grow th associated p ro tein -4 3 , an an tib o d y to outgrow ing n eu ro n al fibres. T he correlative occurrence b e tw e e n extensive sp ro u tin g o f fibres containing calcitonin g en e-related p ep tid e and the rem odeling of necrotic en d o steal bone suggests th a t s e n sory fibres with calcitonin g en e-related p ep tid e have a regulatory role in the control of angiogenesis o r o f b o n e rem odeling associated with th e insertion of an im plant, or with both processes.il i « i r » É ) i n n i r r r o r i i w i * -Î

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Comparison of the immunocytochemical distribution of the phosphoprotein B-50 in the cerebellum and hippocampus of immature and adult rat brain

Cited by 186 publications

References 28 publications

Targeted Overexpression of the Neurite Growth-Associated Protein B-50/GAP-43 in Cerebellar Purkinje Cells Induces Sprouting after Axotomy But Not Axon Regeneration into Growth-Permissive Transplants

Targeted Overexpression of the Neurite Growth-Associated Protein B-50/GAP-43 in Cerebellar Purkinje Cells Induces Sprouting after Axotomy But Not Axon Regeneration into Growth-Permissive Transplants

A morphologically distinct granule cell type in the dentate gyrus of the red fox correlates with adult hippocampal neurogenesis

Changes in innervation of long bones after insertion of an implant: Immunocytochemical study in goats with antibodies to calcitonin gene‐related peptide and B‐50/GAP‐43

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