Comparison of the Immunogenicity of GTPV A27R, GTPV L1R, and SPPV A33R Recombinant Proteins

Production of recombinant lumpy skin disease virus A27L and L1R proteins for application in diagnostics and vaccine development

“…Maximum antibody titres were detected on day 42 post-primary vaccination for all products. These results were similar to immune response profile induced with A27L and L1R from goat poxvirus [38] .…”

“…5 B). Studies that evaluated the diagnostic ability of goatpox virus also reported that A27L had higher detection ability over L1R and A33R [37] , [38] . A comparison of the coating proteins was found to be statistically significant (p-value < 0,0001).…”

Evaluation of antibody responses against the whole virions of goatpox and sheeppox viruses after subcutaneous immunization of rabbits

“…Another report by Thomas Kaever et al (2016), described that inoculation with vaccinia virus (VACV) elicits neutralizing antibodies against major antigens, including A27, A33, B5, D8, H3, and L1 on both the extracellular enveloped virus (EV) and the intracellular mature virion (MV or IMV), conferring protection against smallpox [ 19 ]. Additionally, Hurisa et al (2021), have compared the immunogenicity of three Capripoxvirus proteins; GTPV A 27R, GTPV L1R, and SPPV A 33R proteins, and reported that GTPV A 27R protein showed a better detection ability and can be used for the detection of antibodies produced against Capripoxvirus in sera of animals [ 22 ]. Furthermore, M. Dashprakash et al (2019), reported the diagnostic efficacy of rA27L of GTPV in an indirect ELISA and showed its high specificity, as it was highly reactive with anti-GTPV and anti-SPPV sera but not with antisera against other related viruses of sheep and goats [ 25 ].…”