Comparison of the immunological activities of arabinoxylans from wheat bran with alkali and xylanase-aided extraction

Zhou, Sumei; Liu, Xiuzhen; Guo, Yan; Wang, Qiang; Peng, Daiyin; Cao, Li

doi:10.1016/j.carbpol.2010.03.040

Cited by 159 publications

(138 citation statements)

References 31 publications

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“…A Minimum weight gain was observed in Group E glycosides, terpenoids, phenols, polysaccharides and alkaloids (Wills et al, 2000). A wide range of biological effects have been manifested by cereals such as immunostimulants (Akhtar et al, 2012), antithrombosis, anti-inflammatory, anti-oxidant and anti-stress activities (Zhou et al, 2010). These pronouncements demanded for additional investigations on cereals to explore their immunostimulatory and therapeutic potential against parasitic diseases.…”

Section: Discussionmentioning

confidence: 99%

“…In humans and other animal models, various studies have shown that dietary cereal fibers may have a great effect on different physiological parameters (Neyrink et al, 2008). Cereals based carbohydrates are mainly reported as antitussive, antioxidant, antimutagenic, anti-inflammatory, anticancerous and immunomodulators (Zhou et al, 2010). Cereals, fungi and yeast cell wall contain betaglucans as principle structural component, with molecular weight of 2000 kDa approximately.…”

Section: Introductionmentioning

confidence: 99%

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Immunological and Therapeutic Evaluation of Wheat (Triticum aestivum) Derived Betaglucans against Coccidiosis in Chicken

Rizwan¹,

Khan²,

Iqbal³

et al. 2016

IJAB

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To cite this paper: Rizwan, H.M., M.K. Khan, Z. Iqbal and F. Deeba, 2016. Immunological and therapeutic evaluation of wheat (Triticum aestivum) derived beta-glucans against coccidiosis in chicken. AbstractThis study was carried out to determine the immunomodulatory and therapeutic effects of wheat derived beta-glucans (purified) against avian coccidiosis. Briefly, beta-glucans from wheat bran were extracted and purified using standard procedures. A total of 250 broiler chicks (day-old) were reared at experimental station, Department of Parasitology, University of Agriculture, Faisalabad for this study. At 7 th day of their age, birds were subdivided into five equal groups (n=50). Groups A, B and C were orally administered with graded doses of purified beta-glucans (50, 100 and 150 mg/kg of body weight) for three consecutive days; whereas, positive control (group D) was administered with vitamin-E at the dose rate of 87mg/kg; while negative control (group E) was served with phosphate buffered saline (PBS solution). At 14 th day post administration of graded doses, 30 chickens from each group were randomly selected and used to monitor cellular and humoral immune responses, while remaining birds (n=20) in each group were challenged with mixed species of genus Eimeria for therapeutic evaluation. Overall, chickens in group A administered with 50 mg/kg b.wt. purified beta-glucans showed significantly higher immune responses in terms of enhanced humoral and cell mediated immunity as compared to those of other beta-glucans administered and E groups. While, the immune responses showed by group A were comparable with group D. After challenge with Eimeria, the groups A and D also showed maximum weight gains with low oocyst counts and maximum percent protection against lesions in both caecum and intestine. On the other hand, a minimum daily weight gain with increased number of oocysts in chickens was observed in control group (PBS) during therapeutic evaluation. These findings suggest that the beta-glucans derived from wheat may have immunomodulatory and therapeutic effects against Eimeria infection in chickens.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunological and Therapeutic Evaluation of Wheat (Triticum aestivum) Derived Betaglucans against Coccidiosis in Chicken

Rizwan¹,

Khan²,

Iqbal³

et al. 2016

IJAB

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“…Polysaccharides (AXs) from the de-starched bran were extracted as described by Zhou et al (2010). The fraction thus obtained was used as alkaline extract of AXs and freeze dried for further use.…”

Section: Procurement and Processing Of Oat Bran For Extraction Of Aramentioning

confidence: 99%

Evaluation of Avena sativa Derived Arabinoxylans as Native Biological Response Modifiers in Broilers

Rizwan¹

2017

IJAB

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This study was aimed to assess the immunomodulatory effects of Avena sativa (oat) derived arabinoxylans (AXs) in chickens. The extracted AXs were evaluated for immunological and immunotherapeutic activities against coccidiosis in chickens. Oat derived AXs significantly enhanced (P<0.05) the in vitro and in vivo lympho-proliferative responses to T-cell mitogens in chicken. Moreover, significantly higher (P<0.05) humoral immune retorts in terms of antibody titres (GMT) to sheep red blood cells (SRBCs), Newcastle disease and Infectious bursal disease vaccines were also detected in AXs administered chickens; whereas, the difference in organ-body weight ratios was statistically non-significant between AXs administered and control chickens (P>0.05). Results of the challenge experiment revealed that the daily weight gains and percent protection were statistically higher (P<0.05); while, mean oocysts per gram of droppings and lesion scores were lower (P<0.05) in the AXs administered chickens than the control group. In conclusion, oat derived AXs had immunopotentiating potential that persisted against coccidial infection in chickens.

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“…Usually, the enzymatically extracted AX (Zhou et al 2010) contain arabinose and arabinose linked ferulic acid moieties as substituents on the xylan backbone. However, in our study the alkali extracted purified AX have also shown ferulic acid contents (Table 1) unlike the alkali isolated ones from other sources (Zhou et al 2010). The ferulic acid content was comparatively high (0.11 mg/100 g) in BE as compared to KE1 and KE2.…”

Section: Resultsmentioning

confidence: 99%

“…Concanvalin A (ConA) stimulates T-cell and Lipopolysaccharides (LPS) are usually used to stimulate B-cell proliferation in vivo (Dai et al 2009). However, in vitro assay with wheat bran AX revealed no significant changes in ConA-and LPS-stimulated splenocyte proliferation in previous studies (Zhou et al 2010). Many polysaccharides are known to be T-cell independent B-cell stimulators in producing humoral response in laboratory animals (Taper and Roberfroid 2000;Kayse et al 2003).…”

Section: Mitogenic Activity Of Purified Bran Axmentioning

confidence: 96%

Immunomodulatory activity of purified arabinoxylans from finger millet (Eleusine coracana, v. Indaf 15) bran

2014

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Biological activities of alkali extracted (Barium hydroxide: BE-480 kDa, Potassium hydroxide: KE1-1080 and KE2-40 kDa), purified arabinoxylans (AX) from the finger millet bran varying in their molecular weight, phenolic acid content, arabinose to xylose ratios were evaluated for their immune-stimulatory activities using murine lymphocytes and peritoneal exudate macrophages. All three purified AX displayed significant (p<0.001) mitogenic activity and activation of macrophages including phagocytosis. Among these BE has shown higher enhancing lymphocyte proliferation (>2 fold) and macrophage phagocytosis than KE1 and KE2. The above results clearly documented that the immunostimulatory activity of arabinoxylans is directly proportional to the amount of ferulic acid content (0.11 mg/ 100 g), whereas molecular weight as well as arabinose/ xylose ratio, did not have any bearing. Purified AX from the finger millet bran can be explored as a potent natural immunomodulator.

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Comparison of the immunological activities of arabinoxylans from wheat bran with alkali and xylanase-aided extraction

Cited by 159 publications

References 31 publications

Immunological and Therapeutic Evaluation of Wheat (Triticum aestivum) Derived Betaglucans against Coccidiosis in Chicken

Immunological and Therapeutic Evaluation of Wheat (Triticum aestivum) Derived Betaglucans against Coccidiosis in Chicken

Evaluation of Avena sativa Derived Arabinoxylans as Native Biological Response Modifiers in Broilers

Immunomodulatory activity of purified arabinoxylans from finger millet (Eleusine coracana, v. Indaf 15) bran

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