Comparison of the in vitro effects of saline, hypertonic hydroxyethyl starch, hypertonic saline, and two forms of hydroxyethyl starch on whole blood coagulation and platelet function in dogs

Wurlod, Virginie; Howard, Judith; Francey, Thierry; Schweighauser, Ariane; Adamik, Katja‐Nicole

doi:10.1111/vec.12320

Cited by 36 publications

(67 citation statements)

References 49 publications

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“…Indeed, a 1:22 dilution of whole blood with 7.2% HTS (mimicking a dose of 4 ml/kg) significantly affected Ct PFA and ROTEM® EXTEM® CFT and MCF in dogs [16]. Moreover, 20% mannitol affected Ct PFA and ROTEM® EXTEM® variables to a greater extent than equimolar 3% HTS in dilutions mimicking recommended clinical doses [17].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effects of 7.2% hypertonic saline and 20% mannitol on whole blood coagulation and platelet function in dogs with suspected intracranial hypertension - a pilot study

et al. 2017

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BackgroundHyperosmolar therapy with either mannitol or hypertonic saline (HTS) is commonly used in the treatment of intracranial hypertension (ICH). In vitro data indicate that both mannitol and HTS affect coagulation and platelet function in dogs. The aim of this study was to compare the effects of 20% mannitol and 7.2% HTS on whole blood coagulation using rotational thromboelastometry (ROTEM®) and platelet function using a platelet function analyzer (PFA®) in dogs with suspected ICH. Thirty client-owned dogs with suspected ICH needing osmotherapy were randomized to receive either 20% mannitol (5 ml/kg IV over 15 min) or 7.2% HTS (4 ml/kg IV over 5 min). ROTEM® (EXTEM® and FIBTEM® assays) and PFA® analyses (collagen/ADP cartridges) were performed before (T0), as well as 5 (T5), 60 (T60) and 120 (T120) minutes after administration of HTS or mannitol. Data at T5, T60 and T120 were analyzed as a percentage of values at T0 for comparison between groups, and as absolute values for comparison between time points, respectively.ResultsNo significant difference was found between the groups for the percentage change of any parameter at any time point except for FIBTEM® clotting time. Within each group, no significant difference was found between time points for any parameter except for FIBTEM® clotting time in the HTS group, and EXTEM® and FIBTEM® maximum clot firmness in the mannitol group. Median ROTEM® values lay within institutional reference intervals in both groups at all time points, whereas median PFA® values were above the reference intervals at T5 (both groups) and T60 (HTS group).ConclusionsUsing currently recommended doses, mannitol and HTS do not differ in their effects on whole blood coagulation and platelet function in dogs with suspected ICH. Moreover, no relevant impairment of whole blood coagulation was found following treatment with either solution, whereas a short-lived impairment of platelet function was found after both solutions.

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Section: Discussionmentioning

confidence: 99%

Comparison of the effects of 7.2% hypertonic saline and 20% mannitol on whole blood coagulation and platelet function in dogs with suspected intracranial hypertension - a pilot study

et al. 2017

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“…Some canine studies have suggested that only hypertonic saline, and not hydroxyl starches (HES) (specifically 130/0.4), result in statistically significant changes to platelet function beyond that associated with haemodilution alone (Wurlod et al . , McBride et al . ).…”

Section: Abnormalities Of Platelet Function Resulting In Haemorrhagementioning

confidence: 99%

Abnormal platelet activity in dogs and cats – impact and measurement

Gant

McBride

Humm

2020

J of Small Animal Practice

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Abnormal platelet activity can either lead to bleeding tendencies or inappropriate thrombus formation and can occur secondarily to a wide variety of disease processes, with a range of clinical consequences and severity. This article will discuss the pathophysiology of platelet function abnormalities and consider a logical diagnostic approach applicable to veterinary practice. Recent advances in platelet function testing will then be discussed, with regards to detection of platelet dysfunction and tailoring of pharmacological manipulation. Although many of these tests are still confined to research or academic institutions, techniques for indirectly assessing platelet function are starting to become more widely available. Although we still require further research to develop guidelines for the use of these tests in clinical decision‐making, the recent advances in this field are an exciting step forward in being able to detect and manage platelet dysfunction in both primary care and referral practice.

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“…Two in vitro studies using canine blood reported conflicting conclusions regarding the effect of tetrastarch on platelet function, as measured with a platelet function analyzer . Another recent in vitro study demonstrated impairment in platelet function when canine blood was diluted with both hypertonic hetastarch and hypertonic saline; however, impairment was not noted for dilutions with hetastarch 600/0.75 or hetastarch 130/0.4 …”

Section: Discussionmentioning

confidence: 99%

“…Additionally, an in vivo study in humans undergoing cholecystectomy and treated with hetastarch 130/0.4 demonstrated reduced MCF that was thought to be related to reduced concentrations of fibrinogen . Animal studies have found similar results regarding impairment of MCF as well as effects on other TEM values such as CT, CFT, and alpha‐angle; however, no in vivo studies in dogs have evaluated resuscitative fluid effects on ROTEM whole blood analysis …”

Section: Discussionmentioning

confidence: 99%

“…Recent studies in both human and veterinary medicine have used TEM to evaluate the effects of various fluids on hemostasis. In vitro studies using canine, equine, and human blood have shown that fluids such as hetastarch, a synthetic colloid, and hypertonic saline, a hypertonic crystalloid, affect both viscoelastic coagulation and platelet function, and dilution alone may not explain the changes observed …”

Section: Introductionmentioning

confidence: 99%

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The effect of 3 resuscitative fluid therapies on hemostasis as measured by rotational thromboelastometry in dogs

Seshia

Gaunt

Kidney

et al. 2018

Veterinary Clinical Pathol

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Comparison of the in vitro effects of saline, hypertonic hydroxyethyl starch, hypertonic saline, and two forms of hydroxyethyl starch on whole blood coagulation and platelet function in dogs

Cited by 36 publications

References 49 publications

Comparison of the effects of 7.2% hypertonic saline and 20% mannitol on whole blood coagulation and platelet function in dogs with suspected intracranial hypertension - a pilot study

Comparison of the effects of 7.2% hypertonic saline and 20% mannitol on whole blood coagulation and platelet function in dogs with suspected intracranial hypertension - a pilot study

Abnormal platelet activity in dogs and cats – impact and measurement

The effect of 3 resuscitative fluid therapies on hemostasis as measured by rotational thromboelastometry in dogs

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