Comparison of the incidence of pathogenic and antibiotic-resistant Escherichia coli strains in adult cattle and veal calf slaughterhouse effluents highlighted different risks for public health

Um, Maryse Michèle; Barraud, Olivier; Kérourédan, Monique; Gaschet, Margaux; Stalder, Thibault; Oswald, Éric; Dagot, Christophe; Ploy, Marie-Cécile; Brugère, Hubert; Bibbal, Delphine

doi:10.1016/j.watres.2015.09.029

Cited by 40 publications

(17 citation statements)

References 56 publications

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“…However, the value reached 66% ( stx 1 or stx 2) in a tributary of the Ganges (India) impacted by untreated human sewage (Ram et al, 2009). In comparison, the frequencies of STEC/EHEC reported in the treated effluent of a WWTP connected to a hospital and a slaughterhouse, or in the effluent of a slaughterhouse were 0.2 and 0.5%, respectively (Diallo et al, 2013; Um et al, 2016). The results obtained in sediments are consistent with the study reported by Balière et al (2015), which showed the prevalence of STEC in coastal sediments (0.85%; 1/83 isolated E. coli ) and in freshwater sediments (0.17%; 4/2,036 isolated E. coli ) in a larger coastal watershed in Normandy (1000 km 2 ).…”

Section: Discussionmentioning

confidence: 95%

“…However, within these intra-intestinal pathovars, a very low frequency (0.2%) or no STEC were observed in treated effluent of WWTPs or slaughterhouses (Diallo et al, 2013; Yang et al, 2014; Um et al, 2016). Higher prevalence of EHEC (i.e., presence of stx2 and eae genes) has been reported in lake water in Canada, corresponding to 1.8% (4/658 isolated E. coli ) (Chandran and Mazumder, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Change in the Structure of Escherichia coli Population and the Pattern of Virulence Genes along a Rural Aquatic Continuum

Petit

Clermont

Delannoy³

et al. 2017

Front. Microbiol.

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The aim of this study was to investigate the diversity of the Escherichia coli population, focusing on the occurrence of pathogenic E. coli, in surface water draining a rural catchment. Two sampling campaigns were carried out in similar hydrological conditions (wet period, low flow) along a river continuum, characterized by two opposite density gradients of animals (cattle and wild animals) and human populations. While the abundance of E. coli slightly increased along the river continuum, the abundance of both human and ruminant-associated Bacteroidales markers, as well as the number of E. coli multi-resistant to antibiotics, evidenced a fecal contamination originating from animals at upstream rural sites, and from humans at downstream urban sites. A strong spatial modification of the structure of the E. coli population was observed. At the upstream site close to a forest, a higher abundance of the B2 phylogroup and Escherichia clade strains were observed. At the pasture upstream site, a greater proportion of both E and B1 phylogroups was detected, therefore suggesting a fecal contamination of mainly bovine origin. Conversely, in downstream urban sites, A, D, and F phylogroups were more abundant. To assess the occurrence of intestinal pathogenic strains, virulence factors [afaD, stx1, stx2, eltB (LT), estA (ST), ipaH, bfpA, eae, aaiC and aatA] were screened among 651 E. coli isolates. Intestinal pathogenic strains STEC O174:H21 (stx2) and EHEC O26:H11 (eae, stx1) were isolated in water and sediments close to the pasture site. In contrast, in the downstream urban site aEPEC/EAEC and DAEC of human origin, as well as extra-intestinal pathogenic E. coli belonging to clonal group A of D phylogroup, were sampled. Even if the estimated input of STEC (Shiga toxin-producing E. coli) – released in water at the upstream pasture site – at the downstream site was low, we show that STEC could persist in sediment. These results show that, the run-off of small cattle farms contributed, as much as the wastewater effluent, in the dissemination of pathogenic E. coli in both water and sediments, even if the microbiological quality of the water was good or to average quality according to the French water index.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Change in the Structure of Escherichia coli Population and the Pattern of Virulence Genes along a Rural Aquatic Continuum

Petit

Clermont

Delannoy³

et al. 2017

Front. Microbiol.

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“…A PCR technique to identify the seven phylogenetic groups was recently developed by Clermont et al [ 10 ], and its use is rare in the literature [ 20 – 22 ]. The cited articles in the discussion section of the present study used the triplex PCR-based method, which identifies the phylogroups A, B1, B2, and D. Previously, our group showed the relation of the seven phylogroups according to the host (cattle calves, buffalo calves, and poultry) [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Group of Escherichia coli Isolates from Broilers in Brazilian Poultry Slaughterhouse

Coura

Diniz

Silva

et al. 2017

The Scientific World Journal

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The aim of the study was to determine the phylogenetic groups of E. coli strains isolated from seemingly healthy broiler and broiler condemned suspected of colibacillosis in a Brazilian slaughterhouse. Samples from respiratory tract and edible giblets (liver and heart) of broilers with and without macroscopic lesions of colibacillosis were collected at slaughter. There were 84 strains isolated from broilers condemned of which 11 were obtained from swabs of the heart, 7 from the liver, and 66 from the respiratory tract. Of the 53 E. coli strains isolated from broilers not condemned, 5 were isolated from the heart, 4 from the liver, and 44 from the respiratory tract. E coli strains were tested via PCR for phylogenetic groups A, B1, B2, C, D, E, and F. Phylogroups A and B1 were the most common phylogroups of E. coli obtained from healthy and sick-appearing broiler carcasses. The results of the study showed that phylogroups B2 and E were associated with the heart samples and phylogroup A was associated with respiratory tract samples, phylogroup B1 with not condemned carcass, and phylogroup D with liver samples.

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“…According to Um et al [28], conventional treatment processes have no major impact on the reduction of antibiotic-resistant Escherichia coli strains present in SWW, highlighting the public health risks associated with inadequately treated slaughterhouse effluents concerning the propagation of antibioticresistant and pathogenic bacteria into the environment.…”

Section: Environmental Impact and Health Effects Of Slaughterhouse Wamentioning

confidence: 99%

Slaughterhouse Wastewater: Treatment, Management and Resource Recovery

Bustillo-Lecompte¹,

Mehrvar²

2017

Physico-Chemical Wastewater Treatment and Resource Recovery

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The meat processing industry is one of the largest consumers of total freshwater used in the agricultural and livestock industry worldwide. Meat processing plants (MPPs) produce large amounts of slaughterhouse wastewater (SWW) because of the slaughtering process and cleaning of facilities. SWWs need significant treatment for a sustainable and safe discharge to the environment due to the high content of organics and nutrients. Therefore, the treatment and final disposal of SWW are a public health necessity. In this chapter, the regulatory frameworks relevant to the SWW management, environmental impacts, health effects, and treatment methods are discussed. Although physical, chemical, and biological treatment can be used for SWW degradation, each treatment process has different advantages and drawbacks depending on the SWW characteristics, best available technology, jurisdictions, and regulations. SWWs are typically assessed using bulk parameters because of the various pollutant loads derived from the type and the number of animals slaughtered that fluctuate amid the meat industry. Thus, an on-site treatment using combined processes would be the best option to treat and disinfect the slaughterhouse effluents to be safely discharged into receiving waters.

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Comparison of the incidence of pathogenic and antibiotic-resistant Escherichia coli strains in adult cattle and veal calf slaughterhouse effluents highlighted different risks for public health

Cited by 40 publications

References 56 publications

Change in the Structure of Escherichia coli Population and the Pattern of Virulence Genes along a Rural Aquatic Continuum

Change in the Structure of Escherichia coli Population and the Pattern of Virulence Genes along a Rural Aquatic Continuum

Phylogenetic Group of Escherichia coli Isolates from Broilers in Brazilian Poultry Slaughterhouse

Slaughterhouse Wastewater: Treatment, Management and Resource Recovery

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