Comparison of the influence of EDTA-K3 and sodium citrate on haematology analysis in healthy dogs

Abstract

The study was carried out on 30 clinically healthy dogs of various breeds. Haemoglobin concentration, haematocrit, platelet count and platelet haematocrit were significantly lower in citrate blood than in tripotassium ethylenediaminetetraacetic acid (EDTA-K3) blood. The study confirmed the limited usage of sodium citrate in haematology analysis, unless canine EDTA-dependent thrombocytopenia is suspected.

Variations in the full blood count parameters among apparently healthy humans in the Ho municipality using ethylenediamine tetraacetic acid (EDTA), sodium citrate and lithium heparin anticoagulants: A laboratory-based cross-sectional analytical study

“…They advised that heparin and EDTA were better anticoagulants than sodium citrate because samples anticoagulated with sodium citrate resulted in significant changes in blood parameters. In another study, using blood from healthy dogs, haemoglobin concentration, haematocrit and platelet counts were significantly lower in citrated blood than noted in K 3 EDTA blood [ 13 ] akin to that observed in this study even though this study was conducted using human blood. Research has established the inability of haemoglobin to be oxidized in citrate for which reason lower HGB values are often recorded in citrated blood [ 13 ]; the ripple effect could be the cause of lower HCT values in citrated blood compared to heparinized and K 3 EDTA anticoagulated blood observed in this study.…”

“…In another study, using blood from healthy dogs, haemoglobin concentration, haematocrit and platelet counts were significantly lower in citrated blood than noted in K 3 EDTA blood [ 13 ] akin to that observed in this study even though this study was conducted using human blood. Research has established the inability of haemoglobin to be oxidized in citrate for which reason lower HGB values are often recorded in citrated blood [ 13 ]; the ripple effect could be the cause of lower HCT values in citrated blood compared to heparinized and K 3 EDTA anticoagulated blood observed in this study. In relation to PLT, citrate has been adjudged a strong platelet activator in sick animals, causing the spontaneous formation of PLT microaggregates which often fail recognition by modern automated haematology analyzers [ 20 ].…”

“…Also, platelet activation characterized by abnormally large morphology as well as less granulation in citrated blood has been reported [ 12 ]. Results from 30 clinically healthy dogs concluded limited possibility of using sodium citrate in routine haematological analysis because of its influence on haemoglobin concentration and platelet parameters, including erythrocyte and leukocyte morphology [ 13 ].…”

A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP

“…In our study, we choose a low centrifugation speed (200 g ) at the first centrifugation which could reduce cell pull-down so that the majority of platelets will be left within the plasma, separating from the leukocytes in the buffy coat [ 10 ]. However, under the same centrifugation conditions, c-PRP demonstrated (1) a relatively indistinct boundary and buffy coat, (2) obtaining less quantity of platelets [ 18 ], and (3) a higher level of GFs contents in PRP than plasma, which implies that ACD-A anticoagulants may destroy a part of the blood cells (including red blood cells and platelets). This may be due to a decrease of extracellular calcium ions and pH caused by citrate based anticoagulants, which reduces the stability of the cell membrane and leads to the destruction of senescent blood cells by centrifugal force.…”

“…However, these exogenous additives may have side effects in clinical setting. For example, sodium citrate may cause aggregation of platelets, resulting in inaccurate platelet counts that impact the clinical outcome [ 12 , 18 , 19 ]. Meanwhile, xenogeneic thrombin and other coagulants may trigger immunogenic reactions [ 20 , 21 ].…”