Comparison of the kinetic performance and retentivity of sub-2μm core–shell, hybrid and conventional bare silica phases in hydrophilic interaction chromatography

Evaluation of superficially and fully porous particles for HILIC separation of lanthanide–polyaminocarboxylic species and simultaneous coupling to ESIMS and ICPMS

“…36 The water layer adsorbed at the surface of the stationary phase, combined with the adsorption and/or electrostatic interaction of analytes with the stationary phase, result in decreased diffusivity and greater resistance to the mass transfer, which in turn adversely affect the separation efficiencies. 27,28,37 It must be pointed out that we obtained the same results with the XBridge column packed with FPPs (data not shown), indicating that the decrease in efficiency with the increasing mobile phase ow rate can be assigned to the features of the HILIC mechanism rather than to the particle technology and the support material. Furthermore, we have shown that with amide-bonded stationary phases, 2 an adsorption mechanism played an important role in the HILIC retention of the Ln species, which likely promoted the non-at trend at high ow rates.…”

Study of retention and peak shape in hydrophilic interaction chromatography over a wide pH range

“…Recent studies have confirmed that the mechanism of retention may be solute dependent. For example, the reduced b coefficient in the van Deemter equation determined by peak parking was appreciably smaller for cytosine compared with nortriptyline, the former solute being considerably more hydrophilic [10]. This result was caused by slower effective diffusion relative to bulk diffusion for cytosine, suggesting that it is held in the (more viscous) water layer whereas nortriptyline is held in the adjacent (less viscous) diffuse water layer containing a higher acetonitrile concentration.…”

Recent progress in the fundamental understanding of hydrophilic interaction chromatography (HILIC)

“…63 The superficially porous silica (Cortecs HILIC) was shown to have the lowest plate height (H min ) at higher optical linear velocity. 63 The superficially porous silica (Cortecs HILIC) was shown to have the lowest plate height (H min ) at higher optical linear velocity.…”