Study of retention and peak shape in hydrophilic interaction chromatography over a wide pH range

McCalley, David V.

doi:10.1016/j.chroma.2015.07.092

Cited by 52 publications

(23 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3A). The retention of derivatives in mobile phase containing acetic acid is also influenced by changing ionic strength of the mobile phase, which is very low using acidic additives in solutions with high concentration of acetonitrile [25]. In mobile phase containing ammonium acetate, the retention of non-derivatized oligosaccharides is comparable with mobile phase containing acetic acid, but generally decreases with derivatization of the compounds (Fig.…”

Section: Influence Of the Type Of Derivatization Agent On Retention Omentioning

confidence: 98%

Comparison of isocratic retention models for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides

Česla

Vaňková

Křenková

et al. 2016

Journal of Chromatography A

View full text Add to dashboard Cite

In this work, we have investigated retention of maltooligosaccharides and their fluorescent derivatives in hydrophilic interaction liquid chromatography using four different stationary phases. The non-derivatized maltooligosaccharides (maltose to maltoheptaose) and their derivatives with 2-aminobenzoic acid, 2-aminobenzamide, 2-aminopyridine and 8-aminonaphthalene-1,3,6-trisulfonic acid were analyzed on silica gel, aminopropyl silica, amide (carbamoyl-bonded silica) and ZIC-HILIC zwitterionic sulfobetain bonded phase. The partitioning of the analytes between the bulk mobile phase and adsorbed water-rich layer, polar and ionic interactions of analytes with stationary phase have been evaluated and compared. The effects of the mobile phase additives (0.1% (v/v) of acetic acid and ammonium acetate in concentration range 5-30 mmol L(-1)) on retention were described. The suitability of different models for prediction of retention was tested including linear solvent strength model, quadratic model, mixed-mode model, and empirical Neue-Kuss model. The mixed-mode model was extended to the parameter describing the contribution of monomeric glucose unit to the retention of non-derivatized and derivatized maltooligosaccharides, which was used for evaluation of contribution of both, oligosaccharide backbone and end-group to retention.

show abstract

Section: Influence Of the Type Of Derivatization Agent On Retention Omentioning

confidence: 98%

Comparison of isocratic retention models for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides

Česla

Vaňková

Křenková

et al. 2016

Journal of Chromatography A

View full text Add to dashboard Cite

show abstract

“…Citrate is known to strongly chelate metal oxides in aqueous systems, particularly those of iron(III) [35]. While ethylene diamine tetraacetic acid (EDTA) might under some circumstances be a better complexing agent, it is rather insoluble in HILIC mobile phases containing high proportions of ACN [36]. We postulate that both fluoroquinolones and d-cycloserine undergo chelation-based interactions with labile metal oxides within the chromatographic apparatus.…”

Section: A N U S C R I P Tmentioning

confidence: 99%

Retention characteristics of some antibiotic and anti-retroviral compounds in hydrophilic interaction chromatography using isocratic elution, and gradient elution with repeatable partial equilibration

Heaton

Smith

McCalley

2019

Analytica Chimica Acta

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, strong acids like TFA and heptafluorobutyric acids (HFBA) ammonium salt buffer produced very different selectivity on silica column compared with ammonium formate at similar aqueous pH. Although TFA and HFBA covered a relatively narrow range in water ( w w pH 1.9–2.8), a wider range of true thermodynamic pH in 90% ACN ( s s pH 2.4–5.2) was observed, then TFA and HFBA could give somewhat greater ionic strength, and ammonium salt buffers alternatively maintain much higher ionic strength and give better peak shape . Additionally, increasing the mismatch between the injection solvent (range 95–0% ACN, v/v) and the MP (maintained at 95% ACN, v/v) gave increasing deterioration in peak shape, and magnitude of this effect was much higher than that of increasing injection volume when analysis of basic compounds on a hybrid bare silica phase .…”

Section: Retention Mechanism and Retention Behavior In Hilicmentioning

confidence: 99%

Recent applications of hydrophilic interaction liquid chromatography in pharmaceutical analysis

Zhang

et al. 2016

J of Separation Science

View full text Add to dashboard Cite

Hydrophilic interaction liquid chromatography, an alternative liquid chromatography mode, is of particular interest in separating hydrophilic and polar ionic compounds. Compared with traditional liquid chromatography techniques, hydrophilic interaction liquid chromatography offers specific advantages mainly including: (1) relatively green and water-soluble mobile phase composition, which enhances the solubility of hydrophilic and polar ionic compounds; (2) no need for ion-pairing reagents and high content of organic solvent, which benefits mass spectrometry detection; (3) high orthogonality to reverse-phase liquid chromatography, well adapted to two-dimensional liquid chromatography for complicated samples. Therefore, hydrophilic interaction liquid chromatography has been rapidly developed in many areas over the past decades. This review summarizes the recent progress (from 2012 to July 2016) of hydrophilic interaction liquid chromatography in pharmaceutical analysis, with the focus on detecting chemical drugs in various matrices, charactering active compounds of natural products and assessing biotherapeutics through typical structure unit. Moreover, the retention mechanism and behavior of analytes in hydrophilic interaction liquid chromatography as well as some novel hydrophilic interaction liquid chromatography columns used for pharmaceutical analysis are also described.

show abstract

Study of retention and peak shape in hydrophilic interaction chromatography over a wide pH range

Cited by 52 publications

References 30 publications

Comparison of isocratic retention models for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides

Comparison of isocratic retention models for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides

Retention characteristics of some antibiotic and anti-retroviral compounds in hydrophilic interaction chromatography using isocratic elution, and gradient elution with repeatable partial equilibration

Recent applications of hydrophilic interaction liquid chromatography in pharmaceutical analysis

Contact Info

Product

Resources

About