Comparison of the levels of the growth factors in umbilical cord serum and human milk and its clinical significance

Patki, Satish; Patki, Ujjwala Satish; Patil, Rajendra D.; Somasundaram, Indumathi; Kaingade, Pankaj; Bulbule, Akshata; Nikam, Amar; Pishte, Amit

doi:10.1016/j.cyto.2012.04.010

Cited by 28 publications

(18 citation statements)

References 27 publications

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“…From the results, we prefer that hUCBs could be used as an alternative for FBS in further culture and characterization of eMSCs, or any MSCs. The growth factor secreted from hUCB serum also supports our statement [11]. Besides, this was also supported by Shetty who demonstrates hUCB as an alternative source for FBS [12].…”

Section: Discussionsupporting

confidence: 69%

Marker Profiles of Human Endometrial Stem Cells at Various Passages Cultured In-Vitro

Kaingade

Nikam

Kulkarni

et al. 2017

JSRT

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Endometrium is a dynamic organ, which undergoes extensive proliferation and regeneration throughout reproductive cycle of women. Hence, extensive research on adult stem cells of endometrium is underway. An easy approach to identify stem cells is its marker characterization. However, does these markers behave same in all conditions of stem cells in-vitro is uncertain? Hence, the main objective of the study is to check if mesenchymal stem cells of the endometrium (eMSCs) could retain its marker characterization at various passages in-vitro.The study design was accomplished as follows: The endometrial tissue (n=10) were collected from reproductively active women with a mean age of 35±1.5 and body mass index of 24±1.4, undergoing D&C/hysterectomy. Samples were collected in a sterile manner. The plastic, adherent mesenchymal stem cells obtained from endometrial tissue (eMSCs) are characterized for markers such as CD44, CD166, CD106, CD49d, CD31, CD54, CD34, CD117, CD90, CD105, CD73, CD140b, ABCG2 at various passages (P1, P3, P5 & P10) in-vitro using flowcytometry. The obtained results were analysed statistically using student's t-test and significant results are discussed.The study identified tissue-specific markers of eMSCs, where the percentage expression of most of the markers was consistently similar at P3 and P10, expect for CD49d and CD54, which showed a reduction pattern and ABCG2, which showed an increase in percentage at P10 as compared to P3. This article reveals the significance of in-depth marker characterization of human endometrial stem cells in-vitro.

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Section: Discussionsupporting

confidence: 69%

Marker Profiles of Human Endometrial Stem Cells at Various Passages Cultured In-Vitro

Kaingade

Nikam

Kulkarni

et al. 2017

JSRT

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“…35 An alternative example is vascular endothelial growth factor (VEGF), which is found at concentrations significantly higher in milk than maternal serum, indicating a mammary gland source. 36,37 Understanding the sources of bioactive components of milk also helps to explain the variability in milk concentrations that are observed following maternal use of specific medications (see article in this issue by Rowe, Baker and Hale).…”

Section: Bioactive Components and Their Sourcesmentioning

confidence: 99%

Human Milk Composition

Ballard¹,

Morrow²

2013

Pediatric Clinics of North America

2,016

922

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Synopsis The composition of human milk is the biologic norm for infant nutrition. Human milk also contains many hundreds to thousands of distinct bioactive molecules that protect against infection and inflammation and contribute to immune maturation, organ development, and healthy microbial colonization. Some of these molecules, e.g., lactoferrin, are being investigated as novel therapeutic agents. A dynamic, bioactive fluid, human milk changes in composition from colostrum to late lactation, and varies within feeds, diurnally, and between mothers. Feeding infants with expressed human milk is increasing. Pasteurized donor milk is now commonly provided to high risk infants and most mothers in the U.S. express and freeze their milk at some point in lactation for future infant feedings. Many milk proteins are degraded by heat treatment and freeze-thaw cycles may not have the same bioactivity after undergoing these treatments. This article provides an overview of the composition of human milk, sources of its variation, and its clinical relevance.

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“…Human milk contains specific direct growth factors including platelet-derived growth factor, hepatocyte growth factor (HGF), vascular endothelial growth factor, and insulin (74–76). Each of these is important in angiogenesis, cell development, and tissue proliferation.…”

Section: Anti-inflammatory Factors and Effectsmentioning

confidence: 99%

Innate Immunity and Breast Milk

2017

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Human milk is a dynamic source of nutrients and bioactive factors; unique in providing for the human infant’s optimal growth and development. The growing infant’s immune system has a number of developmental immune deficiencies placing the infant at increased risk of infection. This review focuses on how human milk directly contributes to the infant’s innate immunity. Remarkable new findings clarify the multifunctional nature of human milk bioactive components. New research techniques have expanded our understanding of the potential for human milk’s effect on the infant that will never be possible with milk formulas. Human milk microbiome directly shapes the infant’s intestinal microbiome, while the human milk oligosaccharides drive the growth of these microbes within the gut. New techniques such as genomics, metabolomics, proteomics, and glycomics are being used to describe this symbiotic relationship. An expanded role for antimicrobial proteins/peptides within human milk in innate immune protection is described. The unique milieu of enhanced immune protection with diminished inflammation results from a complex interaction of anti-inflammatory and antioxidative factors provided by human milk to the intestine. New data support the concept of mucosal-associated lymphoid tissue and its contribution to the cellular content of human milk. Human milk stem cells (hMSCs) have recently been discovered. Their direct role in the infant for repair and regeneration is being investigated. The existence of these hMSCs could prove to be an easily harvested source of multilineage stem cells for the study of cancer and tissue regeneration. As the infant’s gastrointestinal tract and immune system develop, there is a comparable transition in human milk over time to provide fewer immune factors and more calories and nutrients for growth. Each of these new findings opens the door to future studies of human milk and its effect on the innate immune system and the developing infant.

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Comparison of the levels of the growth factors in umbilical cord serum and human milk and its clinical significance

Cited by 28 publications

References 27 publications

Marker Profiles of Human Endometrial Stem Cells at Various Passages Cultured In-Vitro

Marker Profiles of Human Endometrial Stem Cells at Various Passages Cultured In-Vitro

Human Milk Composition

Innate Immunity and Breast Milk

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