Comparison of the Oral Microbial Flora of Caries-Free Humans and Conventional Sprague-Dawley Rats

Abstract

In this study, the bacteria from molar dental plaque and scrapings of the tongue of 12 conventional Sprague-Dawley rats on a diet of Purina Rodent Laboratory Chow No. 5001 and tap water were identified at the species or genus level quantitated using anaerobic culture techniques and selected biochemical tests. These results were compared to the microbial composition of dental plaque and scrapings from dental and tongue sites, evaluated by similar cultural techniques, of 7 caries-free students on a low-sugar diet for 3 weeks. Similarities did exist between the human and oral microflora, which included the percentage of gram-positive cocci, gram-negative cocci, Streptococcus mutans, and Streptococcus sanguis recovered at the dental sires. Differences from the human included the percentage of Actinomyces species, coliforms, enterococci, and Veillonella species recovered from the dental sites. The results suggest that the oral microflora of Sprague-Dawley rats should be considered whe model is used for human oral disease studies.

Comparative Microbiological and Immunological Studies of Subgingival Dental Plaque from Man and Baboons

“…The low recovery of Gram-negative cocci (Veillonella and Neisseria species) has been explained in other animal models by their poor adherence to oral surfaces and pre-formed dental plaque (Liljemark and Gibbons, 1971;Hall et al, 1984). Anaerobic Gram-positive rods of the oral flora in man were not identified in baboons.…”

“…The facultative anaerobic members were Escherichia coli, Klebsiella ozaenae, and Citrobacter freundii. All have been identified as dominant species of the Gram-negative bacilli in rats (Rosebury et al, 1934;Hall et al, 1984), but are infrequently found in humans (Hardie, 1976). Among the Gram-positive cocci, the cariogenic mannitol-positive S. mutans (serotype h) was isolated.…”

Studies on caries occurrence in rats with naturally occurring dental caries.

“…When a cariogenic diet was used, the rats' general health was affected in many ways, as follows15,16): (1) the molar teeth were smaller and weighed less than normal, (2) incisor teeth lost the usual orange pigmentation and the mandibule and femur were smaller in size than normal, (3) the weight gained was less than normal, (4) protein-calorie levels in the bones and teeth of the rats were deficient. Studies using animal models are necessary and effective, for human caries research Hall et al 22) investigated similarities of oral microflora. It is suggested that a strain of rats with naturally occurring dental caries is a more appropriate model to research on human dental caries.…”

Η Επιδραση Της Ξυλιτολης Της Σορβιτολης Και Του Μιγματος Αυτων Στη Τερηδονογονο Και Οξεογονο Δυναμη Του Αμυλου Και Του Ψωμιου

“…ΠοΧΧά σχελέχη στρεπτόκοκκων μπορούν να μεχαβολίσουν χη σουκρόζη, Ο Str. mutans, όμιυς, αποτελεί τον πλέον χερηδονογόνο μικροοργανισμό της πλάκας και είναι εξαρτώμενος από χη σουκρόζη (Hamada & Slade, 1980;Loesche, 1985).…”

“…από όλες τις άλλες, εξακολουθώντας όμως να είναι σε χαμηλό επίπεδο και πολύ κοντά, στο ποσοστό των Str. mutans της φυσιολογικής χλωρίδας των επιμϋων (Hall et al, 1984). Ενδει κτικά αναφέρουμε οτι η διατροφή με 56/.…”