Comparison of the Performance of Urinary<i> Mycobacterium tuberculosis</i> Antigens Cocktail (ESAT6, CFP10, and MPT64) with Culture and Microscopy in Pulmonary Tuberculosis Patients

Turbawaty, Dewi Kartika; Sugianli, Adhi Kristianto; Soeroto, Arto Yuwono; Setiabudiawan, Budi; Parwati, Ida

doi:10.1155/2017/3259329

Cited by 16 publications

(9 citation statements)

References 25 publications

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“…A higher sensitivity of 71% was found in a study involving adult population, using TB antigens cocktail, ESAT-6, CFP-10, and MPT-64 rapid immunochromatography test (21). However, it remained unclear whether it was due to the multiple specific antigens used in the cocktail (compared to a single antigen in our study) or higher concentration of CFP-10 in urinary specimens of adult TB patients, which typically showing more positive results on M.tb culture.…”

Section: Discussionmentioning

confidence: 90%

“…However, due to lower specificity, it was not meant to replace the conventional microscopic examination and M.tb culture. To increase the sensitivity and positive predictive value of urinary CFP-10 level, it should be used in conjunction with microbiological examination (21). A positive result on urinary CFP-10 levels would provide better diagnostic predictive value in both clinically and microbiologically confirmed TB cases than those only microbiologically confirmed.…”

Section: Discussionmentioning

confidence: 99%

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The diagnostic value of urinary culture filtrate protein-10antigen in childhood tuberculosis

Iskandar¹,

Arthamin²,

Kusdinar³

et al. 2021

Biomedicine

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Introduction and Aim:Childhood tuberculosis (TB) remains a major problem worldwide.However, diagnosis of tuberculosis in children is often complicated by the difficulty in obtaining a proper sputum specimens and low sensitivity of the gold standard diagnostic test to confirm the presence of Mycobacterium tuberculosis(M.tb)in this age group. Recently, M.tbantigen detection in urinaryspecimenshas become a popular method. It is non-invasiveand handling of specimen is simple. It was reported that urinary CFP-10, a specific protein of M.tb, has emerged as a potential biomarker in the future. However, its diagnostic value as a new biomarker in childhood TB remains poorly understood.The aim of the study is to determine the diagnostic value of urinary CFP-10 in childhood TB. Methods: Seventy children with suspected pulmonary or extrapulmonary TB were enrolled. Tuberculosis was diagnosed by performingTuberculin skin test, chest x-ray, microscopic examination, and microbiological cultureobtainedfrom sputum or gastric lavage specimen. The level ofurinary CFP-10 antigen was analyzedbyELISA (Elabscience, China). Statistical analyseswereperformed using SPSS 21.0 and p-values of <0.05 were consideredstatistically significant. Results: The levels of urinary CFP-10 in subjects diagnosed with TB was higher than that of the non-TB subjects, 4.13(0.62) vs 0.43(0.14) pg/mL, p=0.005. The cut-off value forurinary CFP-10 level reached 0.39 pg/mL (sensitivity 65% and specificity 67%). This value became0.54 pg/mL (sensitivity 61% and specificity 62%)in microbiologically confirmed cases. Conclusion: The urinary CFP-10 level has moderate diagnostic value for diagnosing childhood TB.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

The diagnostic value of urinary culture filtrate protein-10antigen in childhood tuberculosis

Iskandar¹,

Arthamin²,

Kusdinar³

et al. 2021

Biomedicine

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“…In fact, I-PCR comprises the versatility of ELISA with the enormous amplification capacity of PCR, thereby exhibiting many-fold higher sensitivity than ELISA [17]. Moreover, urine is quite easy to obtain that has a lesser risk of infection to the laboratory workers but it carries low concentration of M. tuberculosis biomarkers [18]. Detection of mycobacterial lipoarabinomannan (LAM) within urine samples is considered as an attractive biomarker, which has been demonstrated in HIV-coinfected and HIVuninfected TB individuals by lateral flow immunochromatography and ELISA methods though poor sensitivities were observed in HIV-uninfected TB individuals [17,19].…”

Section: Immuno-pcrmentioning

confidence: 99%

Recent Trends in Diagnosis of Urogenital Tuberculosis

Mehta

Kamra

2020

Future Microbiol.

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the efficient diagnosis of UGTB by reliable tests would certainly reduce the infertility in both men and women and the other sequelae associated with disease. " Urogenital tuberculosis (UGTB) is the second most common form of extrapulmonary TB (EPTB) in developing nations with severe epidemic situations. For example, India (comprising 45% of EPTB cases) and the third most common form in locations with a lower incidence of TB [1,2], which include both renal and genital TB. However, concomitant UGTB and pulmonary TB (PTB) are found in 2-10 and 15-20% of patients in developed and developing nations, respectively [2]. In fact, UGTB occurs after a prolonged period of latent infection followed by hematogenous spread to the kidneys, fallopian tubes and epididymis [3]. The clinical manifestations of female UGTB include primary or secondary infertility, menstrual dysfunction and pelvic inflammatory disease [4]. Infertility is the most common complication, which occurs in 5-16% of Indian women caused by irreversible damage to the fallopian tubes. Mostly, fallopian tubes are associated with congestion and miliary tubercles, while endometrium is linked with caseation and ulceration in 50-80% cases, resulting in Asherman's syndrome [5]. Similarly, the most frequent sites for male UGTB are the epididymis and prostate followed by seminal vesicles and testicles originating from renal foci, which also lead to infertility [6]. Owing to asymptomatic nature and varied clinical presentations (ranging from vague urinary symptoms to chronic kidney disease) that mimic several urologic and genital diseases, diagnosis of UGTB is a daunting challenge [3].Smear/culture, histopathology, interferon-γ release assays (IGRAs), intravenous pyelography, laparoscopy and nucleic acid amplification tests (NAATs) are the main modalities employed in the diagnosis of UGTB [6][7][8], which have certain limitations. Smear microscopy and culture for Mycobacterium tuberculosis identification (on Lowenstein-Jensen medium or BACTEC-460/MGIT-960) are commonly used methods but they lead to poor sensitivities due to paucibacillary nature of specimens [7]. Though urine culture is considered the gold standard, it requires skillful technicians and has a prolonged turnaround time of approximately 6-8 weeks [7] when irreparable damage to the fallopian tubes and epididymis already occurs. Delayed diagnosis also leads to nonfunctioning unilateral kidney, contracted bladder and renal failure [1]. Endoscopic procedures such as laparoscopy/hysteroscopy are often linked with operative risks and mostly cause flaring of infection [5], while histopathological examination can be confirmatory only when it demonstrates acid-fast bacilli in the tissues that are very scarce in endometrial biopsies (EBs) [7]. Intravenous pyelography may be suggestive of UGTB but it is nonspecific and often mimics other pathologic lesions [1]. IGRAsImmunological procedures such as IGRAs are widely employed for the early detection of UGTB cases. Notably, Kim et al. [8] reported a sensitivity of 52.6%...

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“…In one study, Turbawaty et al attempted to detect the presence of all the three antigens in urine using a cocktail of polyclonal antibodies against all the three antigens. The authors showed that this strategy increased the sensitivity to 90%, although the specificity remained poor at <30% (Turbawaty et al, 2017).…”

Section: Prospects Of Detecting Miscellaneous Components Of Mtbmentioning

confidence: 99%

Immune Biomarkers for Diagnosis and Treatment Monitoring of Tuberculosis: Current Developments and Future Prospects

et al. 2019

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Tuberculosis (TB) treatment monitoring is paramount to clinical decision-making and the host biomarkers appears to play a significant role. The currently available diagnostic technology for TB detection is inadequate. Although GeneXpert detects total DNA present in the sample regardless live or dead bacilli present in clinical samples, all the commercial tests available thus far have low sensitivity. Humoral responses against Mycobacterium tuberculosis (Mtb) antigens are generally low, which precludes the use of serological tests for TB diagnosis, prognosis, and treatment monitoring. Mtb-specific CD4 + T cells correlate with Mtb antigen/bacilli burden and hence might serve as good biomarkers for monitoring treatment progress. Omics-based techniques are capable of providing a more holistic picture for disease mechanisms and are more accurate in predicting TB disease outcomes. The current review aims to discuss some of the recent advances on TB biomarkers, particularly host biomarkers that have the potential to diagnose and differentiate active TB and LTBI as well as their use in disease prognosis and treatment monitoring.

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Comparison of the Performance of Urinary Mycobacterium tuberculosis Antigens Cocktail (ESAT6, CFP10, and MPT64) with Culture and Microscopy in Pulmonary Tuberculosis Patients

Cited by 16 publications

References 25 publications

The diagnostic value of urinary culture filtrate protein-10antigen in childhood tuberculosis

The diagnostic value of urinary culture filtrate protein-10antigen in childhood tuberculosis

Recent Trends in Diagnosis of Urogenital Tuberculosis

Immune Biomarkers for Diagnosis and Treatment Monitoring of Tuberculosis: Current Developments and Future Prospects

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