The aim of this research is to measure and analyze the effect of ladder training on increasing the agility of karate athletes aged 14 -16 years. This type of research is experimental research using pre-test and post-test. The agility test used in this research was the hexagonal agility test. The sample was 40 karate athletes with an average age of 15.1 ± 0.7 years and training experience of 4.1 ± 5.4 years. There were 2 groups, namely the control group and the training group using a ladder drill. This exercise was carried out 18 times with 3 meetings a week. The results of this study showed no effect on agility in the control group 0.049 (p<0.005) with a time of 17.50 to 17.23 seconds, the average increase was 0.27 seconds and the effect was 37.8%. Meanwhile, there was an influence on agility in the ladder drill training group of 0.003 (p<0.005) with a time of 17.48 to 16.47 seconds, the average increase was 1.1 seconds and had an influence of 72%. The importance of this training model can provide variations for coaches to train athletes so that athletes' agility can develop and athletes can develop strategies and tactics during kumite.