Comparison of the Physical Maps of the DNAs of Two Cytomegalovirus Strains

Weststrate, M. W.; Geelen, J. L. M. C.; Wertheim, Pauline; Noordaa, J. van der

doi:10.1099/0022-1317-64-1-47

Cited by 14 publications

(10 citation statements)

References 6 publications

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“…This is in line with the previous finding that the ends of the L and S components of HCMV tend to be deleted without affecting infectivity (Weststrate et al, 1983;Furukawa, 1984). The pathogenicity and genetics of HCMV strain Tanaka in connection with its peculiar genome structure will be the subjects of future studies.…”

Section: Discussionsupporting

confidence: 87%

A New Human Cytomegalovirus Isolate Has an Invertible Subsegment within Its L Component Producing Eight Genome Isomers

Takekoshi

Ihara

Tanaka

et al. 1987

Journal of General Virology

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SUMMARYA HindIII cleavage map of the genome DNA of a new isolate of human cytomegalovirus (HCMV), strain Tanaka, was constructed by cosmid cloning and Southern blot hybridization of virion DNA. The genome was found to be unique in that its long (L) component was composed of two subsegments, L1 and L2, and subsegment L2 underwent inversion relative to L1 at high frequency. In addition to the normal inversions of the L and short (S) components, this produced eight genome isomers. The novel invertible subsegment was flanked by an inverted sequence distinct from the inversion-specific a sequence present in the terminal and junction regions of the genome.

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Section: Discussionsupporting

confidence: 87%

A New Human Cytomegalovirus Isolate Has an Invertible Subsegment within Its L Component Producing Eight Genome Isomers

Takekoshi

Ihara

Tanaka

et al. 1987

Journal of General Virology

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“…The genomes of human cytomegalovirus (HCMV) strains (defined in Table 1) are highly related as judged by reassociation kinetics [3,4] and Southern analyses [3,[5][6][7]. Different strains of HCMV, however, are readily distinguishable from one another by comparing patterns of restriction enzyme digested viral DNA.…”

Section: Introductionmentioning

confidence: 99%

A review of genetic differences between limited and extensively passaged human cytomegalovirus strains

Prichard¹,

Penfold

Duke³

et al. 2001

Reviews in Medical Virology

166

134

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The complete genetic content of human cytomegalovirus (HCMV) has been difficult to determine, since most strains studied in the laboratory have been extensively passaged in human fibroblast cultures which can change the genetic content as well as the biological properties of the virus. Approximately 13 kb of novel DNA sequences located near the right edge of the unique long (UL) component of the genome has been discovered in Toledo, clinical isolates and certain stocks of Towne. This region of novel sequence, designated the UL/b' region, encodes several interesting proteins including vCXC-1, a potent IL-8 homologue, and UL144, a member of the TNF receptor family. This region is missing from the prototypic laboratory variants of Towne and AD169. In contrast to Toledo and other low passage isolates which have relatively small repeats bracketing the UL component, the Towne and AD169 laboratory variants contain large (>10 kb) b/b' repeats. The large size of these repeats in AD169 and Towne appear to have arisen as compensation for the loss of sequences from the UL/b' region that existed in less passaged variants of these strains. Consequently, many of the haploid genes at the left edge of the prototypic wild-type (wt) UL component are diploid in AD169 and Towne. We hypothesise that this plasticity of the genome at the right edge of the UL component results from extensive passage and adaptation to replication in fibroblasts in vitro. Further work will be required to understand the complete genetic content of wt HCMV.

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“…DISCUSSION Analysis of the genomes of four strains of HCMV by DNA-DNA hybridization with cloned subgenomic fragments of strain AD169 has allowed us to ascertain that the genomes of these HCMV strains are colinear with that of AD169 used as a reference strain. This notion was first suggested by LeFemina & Hayward (1980) and later by Weststrate et al (1983). The latter authors compared the BglII, HindIII and XbaI maps of the AD169 and SG strains by crosshybridization.…”

Section: Hybridization With Xbai Digests Of Hcmv Dnamentioning

confidence: 87%

“…It has been suggested that some digestion fragments have the same size and map position in the UL and Us regions of the genome. LaFemina & Hayward (1980) and later Weststrate et al (1983) suggested that HCMV genomes might be colinear. There appear to be size differences in the repeat regions (Geelen & Weststrate, 1982;Tamashiro et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Demonstration of the Colinearity of Human Cytomegalovirus Genomes and Construction of Restriction Maps of Unknown Isolates Using Cloned Subgenomic Fragments

Colimon

Somogyi

Bertrand

et al. 1985

Journal of General Virology

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SUMMARYIn this study, we have established the colinearity of human cytomegalovirus (HCMV) genomes using stringent conditions of DNA-DNA filter hybridization of HCMV HindIII fragments and cosmid-cloned AD169 strain HCMV DNA fragments. Large cosmid-cloned fragments of AD169 DNA were used for the preparation of radioactive probes by nick translation. These probes were hybridized to HindIII digests of DNA from three fresh isolates of HCMV and to that of the Davis strain. Using published HindIII restriction maps for the AD169 strain as a reference, the results obtained by hybridization allowed us to construct HindIII restriction maps for the genomes of the three fresh isolates. Confirmation of our methodology was found in the correspondence between the HindIII map we constructed for the Davis strain and that published previously. Furthermore, it is shown that variation in the restriction profiles of the unique regions of the genome are due to the absence or gain of restriction sites, and not to a rearrangement of fragments. This technique allows rapid construction of physical maps of the DNA of any fresh isolate for a given restriction enzyme provided the corresponding restriction map of a strain to be used as reference is available.

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Comparison of the Physical Maps of the DNAs of Two Cytomegalovirus Strains

Cited by 14 publications

References 6 publications

A New Human Cytomegalovirus Isolate Has an Invertible Subsegment within Its L Component Producing Eight Genome Isomers

A New Human Cytomegalovirus Isolate Has an Invertible Subsegment within Its L Component Producing Eight Genome Isomers

A review of genetic differences between limited and extensively passaged human cytomegalovirus strains

Demonstration of the Colinearity of Human Cytomegalovirus Genomes and Construction of Restriction Maps of Unknown Isolates Using Cloned Subgenomic Fragments

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